BIO 340 MIDTERM EXAM -
COMPLETE QUESTIONS AND DETAILED SOLUTIONS
LATEST UPDATE THIS YEAR JUST RELEASED
Question 1: A bacterial enzyme that cuts dna within a short,
specific sequence and is used in many applications by the
molecular biologist is known as:
Answer:
restriction enzymes
Question 2: Which of the following is not needed in order to amplify
a dna fragment by pcr
Answer:
knowledge of the complete nucleotide sequence of the fragment to be
amplified
Question 3: Which of the following best describes the complete
sequence of steps occurring every cycle of pcr?
Answer:
the primers hybridize to the target dna
Question 4: Which is the function of dideoxynucelotides in sanger
dna sequencing?
Answer:
they stop synthesis at a specific site, so the base at that site can be
determined
Question 5: Illumina next generation sequencing uses...
, Answer:
light detection after each round of synthesis
Question 6: Which of the following is an advantage of rna
sequencing over microarray analysis when looking at the mrna
expression levels within a population of cells?
Answer:
you can quantify all the rna, rather than just the previously defines
transcripts genes that you have probes for
Question 7: Which of the following methods provides the most
sensitive and accurate way of measuring and comparing mrna
levels of a small set of genes in two different tissues. The genes are
expressed at relatively low levels in these tissues, and do not
necessarily code for protien
Answer:
rt-pcr
Question 8: Which of the following techniques would you use to
see where a particular rna molecular localized within a cell after
you edited its 3'utr
Answer:
in situ hybridization
Question 9: Which of the following techniques would be the most
appropriate use to see when activation of a particular promoter
causes a protein to be translated ?
Answer:
reporter gene
COMPLETE QUESTIONS AND DETAILED SOLUTIONS
LATEST UPDATE THIS YEAR JUST RELEASED
Question 1: A bacterial enzyme that cuts dna within a short,
specific sequence and is used in many applications by the
molecular biologist is known as:
Answer:
restriction enzymes
Question 2: Which of the following is not needed in order to amplify
a dna fragment by pcr
Answer:
knowledge of the complete nucleotide sequence of the fragment to be
amplified
Question 3: Which of the following best describes the complete
sequence of steps occurring every cycle of pcr?
Answer:
the primers hybridize to the target dna
Question 4: Which is the function of dideoxynucelotides in sanger
dna sequencing?
Answer:
they stop synthesis at a specific site, so the base at that site can be
determined
Question 5: Illumina next generation sequencing uses...
, Answer:
light detection after each round of synthesis
Question 6: Which of the following is an advantage of rna
sequencing over microarray analysis when looking at the mrna
expression levels within a population of cells?
Answer:
you can quantify all the rna, rather than just the previously defines
transcripts genes that you have probes for
Question 7: Which of the following methods provides the most
sensitive and accurate way of measuring and comparing mrna
levels of a small set of genes in two different tissues. The genes are
expressed at relatively low levels in these tissues, and do not
necessarily code for protien
Answer:
rt-pcr
Question 8: Which of the following techniques would you use to
see where a particular rna molecular localized within a cell after
you edited its 3'utr
Answer:
in situ hybridization
Question 9: Which of the following techniques would be the most
appropriate use to see when activation of a particular promoter
causes a protein to be translated ?
Answer:
reporter gene
