MOLECULAR DIAGNOSTICS 3RD EDITION
COMPREHENSIVE SOLVED QUESTIONS AND
COMPLETE ANSWERS
◉ What does a mutation refer to?
Answer: Permanent alteration in DNA sequence
◉ Which laboratory area is MOST prone to contamination by
amplified DNA products?
Answer: Post-amplification area
◉ What is the primary purpose of quality control in a molecular
laboratory?
Answer: Ensure reliable results
◉ Which of the following is considered a nucleic acid?
Answer: DNA
◉ What is the storage condition commonly used for long-term
preservation of DNA samples?
Answer: −20°C to −80°C
, ◉ Which molecular technique revolutionized molecular diagnostics
by allowing amplification of DNA?
Answer: PCR
◉ What best describes molecular diagnostics?
Answer: Uses nucleic acids to detect disease
◉ What is hybridization best defined as?
Answer: Binding of complementary nucleic acid strands
◉ Which base pair is formed during DNA hybridization?
Answer: A-T
◉ What principle is the foundation of molecular hybridization?
Answer: Complementary base pairing
◉ What is a nucleic acid probe?
Answer: A fragment of nucleic acid used to detect specific sequences
in nucleic acids.
◉ What is hybridization dependent on?
Answer: Complementary base pairing
COMPREHENSIVE SOLVED QUESTIONS AND
COMPLETE ANSWERS
◉ What does a mutation refer to?
Answer: Permanent alteration in DNA sequence
◉ Which laboratory area is MOST prone to contamination by
amplified DNA products?
Answer: Post-amplification area
◉ What is the primary purpose of quality control in a molecular
laboratory?
Answer: Ensure reliable results
◉ Which of the following is considered a nucleic acid?
Answer: DNA
◉ What is the storage condition commonly used for long-term
preservation of DNA samples?
Answer: −20°C to −80°C
, ◉ Which molecular technique revolutionized molecular diagnostics
by allowing amplification of DNA?
Answer: PCR
◉ What best describes molecular diagnostics?
Answer: Uses nucleic acids to detect disease
◉ What is hybridization best defined as?
Answer: Binding of complementary nucleic acid strands
◉ Which base pair is formed during DNA hybridization?
Answer: A-T
◉ What principle is the foundation of molecular hybridization?
Answer: Complementary base pairing
◉ What is a nucleic acid probe?
Answer: A fragment of nucleic acid used to detect specific sequences
in nucleic acids.
◉ What is hybridization dependent on?
Answer: Complementary base pairing
