MOLECULAR DIAGNOSTICS 3RD EDITION
ACTUAL TEST PAPER QUESTIONS AND
SOLUTIONS GRADED A+
◉ Both consist of a sugar, phosphate residue, and a purine or
pyrimidine base.
Answer: Which of the following characteristics are common to both
RNA and DNA?
a. Both exist primarily as single-stranded molecules
b. Both consist of a sugar, phosphate residue, and a purine or
pyrimidine base
c. Both contain the pyrimidine uracil
d. Both are linked by disulfide bonds
◉ Repeat the entire run.
Answer: If you see amplification in the negative control on a PCR,
what should you do next?
a. Repeat all negative samples
b. Repeat the entire rune
c. Repeat all positive samples
,d. Repeat only low positive samples
◉ PCR testing.
Answer: A physician called the microbiology laboratory and asked
what he needed to order to quickly diagnose Bordetella pertussis
from a child who had what appeared to be having whooping cough.
Which of the following should the physician be told to order?
a. Direct fluorescent antibody (DFA) test only
b. Culture only
c. PCR testing
d. IgG and IgM antibody tests
◉ The procedure requires endpoint measurement that may not be in
direct proportion to the initial sample.
Answer: Which is the main limitation of standard PCR that is not a
limitation of real-time PCR?
a. Requirement of a large initial sample
b. The procedure requires endpoint measurement that may not be in
direct proportion to the initial sample
c. Ability to detect PCR product during the plateau stage
d. Slow turnaround time compared to culture methods
,◉ Capillary electrophoresis (CE).
Answer: In DNA sequencing, multiple fluorescence labeled
nucleotides are commonly separated using what type of test?
a. Serum protein electrophoresis (SPEP)
b. Capillary electrophoresis (CE)
c. Two-dimensional electrophoresis
d. HPLC
◉ Within the log-linear phase.
Answer: In what portion of the amplification curve (logarithmic
plot) should the threshold be set for a real-time PCR?
a. At the baseline
b. Within the log-linear phase
c. At the plateau
d. At the last cycle
◉ Echinococcus granulosus - routine ova and parasite examination.
Answer: All of the following represents an organism paired with its
appropriate diagnostic procedure, EXCEPT:
, a. Trichinella spiralis - serologic testing
b. Cryptosporidium - modified acid-fast stain
c. Echinococcus granulosus - routine ova and parasite examination
d. Schistosoma haematobium - examination of urine sediment
◉ Determine the degree of immune suppression caused by HIV.
Answer: All of the following are uses of nucleic acid tests for HIV
infection, EXCEPT?
a. Monitory the effectiveness of antiretroviral therapy
b. Obtain a baseline value prior to the start of antiretroviral therapy
c. Diagnose HIV infection
d. Determine the degree of immune suppression caused by HIV
◉ Responsible for transportation of information - DNA.
Answer: All of the following statements are correctly matched with
their corresponding nucleic acid, EXCEPT:
a. Responsible for amino acid transport - tRNA
b. Location of protein synthesis - rRNA
ACTUAL TEST PAPER QUESTIONS AND
SOLUTIONS GRADED A+
◉ Both consist of a sugar, phosphate residue, and a purine or
pyrimidine base.
Answer: Which of the following characteristics are common to both
RNA and DNA?
a. Both exist primarily as single-stranded molecules
b. Both consist of a sugar, phosphate residue, and a purine or
pyrimidine base
c. Both contain the pyrimidine uracil
d. Both are linked by disulfide bonds
◉ Repeat the entire run.
Answer: If you see amplification in the negative control on a PCR,
what should you do next?
a. Repeat all negative samples
b. Repeat the entire rune
c. Repeat all positive samples
,d. Repeat only low positive samples
◉ PCR testing.
Answer: A physician called the microbiology laboratory and asked
what he needed to order to quickly diagnose Bordetella pertussis
from a child who had what appeared to be having whooping cough.
Which of the following should the physician be told to order?
a. Direct fluorescent antibody (DFA) test only
b. Culture only
c. PCR testing
d. IgG and IgM antibody tests
◉ The procedure requires endpoint measurement that may not be in
direct proportion to the initial sample.
Answer: Which is the main limitation of standard PCR that is not a
limitation of real-time PCR?
a. Requirement of a large initial sample
b. The procedure requires endpoint measurement that may not be in
direct proportion to the initial sample
c. Ability to detect PCR product during the plateau stage
d. Slow turnaround time compared to culture methods
,◉ Capillary electrophoresis (CE).
Answer: In DNA sequencing, multiple fluorescence labeled
nucleotides are commonly separated using what type of test?
a. Serum protein electrophoresis (SPEP)
b. Capillary electrophoresis (CE)
c. Two-dimensional electrophoresis
d. HPLC
◉ Within the log-linear phase.
Answer: In what portion of the amplification curve (logarithmic
plot) should the threshold be set for a real-time PCR?
a. At the baseline
b. Within the log-linear phase
c. At the plateau
d. At the last cycle
◉ Echinococcus granulosus - routine ova and parasite examination.
Answer: All of the following represents an organism paired with its
appropriate diagnostic procedure, EXCEPT:
, a. Trichinella spiralis - serologic testing
b. Cryptosporidium - modified acid-fast stain
c. Echinococcus granulosus - routine ova and parasite examination
d. Schistosoma haematobium - examination of urine sediment
◉ Determine the degree of immune suppression caused by HIV.
Answer: All of the following are uses of nucleic acid tests for HIV
infection, EXCEPT?
a. Monitory the effectiveness of antiretroviral therapy
b. Obtain a baseline value prior to the start of antiretroviral therapy
c. Diagnose HIV infection
d. Determine the degree of immune suppression caused by HIV
◉ Responsible for transportation of information - DNA.
Answer: All of the following statements are correctly matched with
their corresponding nucleic acid, EXCEPT:
a. Responsible for amino acid transport - tRNA
b. Location of protein synthesis - rRNA
