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1. A patient with multiple myeloma has the following A
reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency
2. An antibody screen utilizing polyethylene glycol C
(PEG) was performed. Reaction readings of this an-
tibody screen are analyzed after immediate spin and
again at 37°C incubation with subsequent centrifuga-
tion. How will the centrifugation of the tubes affect the
patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen
3. What must be true for the antiglobulin phase of the A
serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is
not history of detection of unexpected antibodies.
B. The patient has not been transfused within the past
24 hours.
C. The blood is needed for surgery.
D. There is a history of detection of unexpected anti-
bodies but the current antibody screen is negative.
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4. Which of the following accurately represents reverse B
typing?
A. Reverse typing is performed using known reagent
antisera to detect ABO antigens on the patient's red
blood cells.
B. Reverse typing is performed using known reagent
red blood cells to detect ABO antibodies in the pa-
tient's serum or plasma.
C. Reverse typing is performed using known reagent
screening cells to detect clinically significant antibod-
ies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent
antisera to phenotype donor red blood cells for clini-
cally significant antigens.
5. A former patient had an anti-E four years ago, but her B
antibody panel is now negative. Since she now needs
blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units.
6. What is the maximum interval during which a recipient B
sample may be used for compatibility testing if the
patient has recently been transfused or was pregnant
within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks
7. A patient transfused with two units of packed cells D
spiked a fever of 99.5oF and complained of chills
five days after transfusion. The direct antiglobulin test
(DAT) was positive with anti-IgG, but negative with
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anti-C3d. Compatibility testing was performed on the
pre- and post-transfusion specimens. The post-trans-
fusion specimen was incompatible with one of the
donor units transfused. An antibody screen was done
on both the pre- and post-transfusion specimens. An
antibody was detected in the post-transfusion speci-
men only and identified by panel studies as anti-Jka.
This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction
8. You perform a gel ABO/Rh test on a 45-year-old male D
patient and you obtain the following results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS
9. When performing an anti-human globulin (AHG) test, D
it is important to completely wash the red cells be-
cause:
A. Washing eliminates concentrations of unbound
antigens.
B. Washing prevents elution of cell-bound antibody.
C. Washing promotes false positive effects of
Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf
Blood Bank - Serologic and Molecular Testing
Study online at https://quizlet.com/_fsmdf5
1. A patient with multiple myeloma has the following A
reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency
2. An antibody screen utilizing polyethylene glycol C
(PEG) was performed. Reaction readings of this an-
tibody screen are analyzed after immediate spin and
again at 37°C incubation with subsequent centrifuga-
tion. How will the centrifugation of the tubes affect the
patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen
3. What must be true for the antiglobulin phase of the A
serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is
not history of detection of unexpected antibodies.
B. The patient has not been transfused within the past
24 hours.
C. The blood is needed for surgery.
D. There is a history of detection of unexpected anti-
bodies but the current antibody screen is negative.
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, Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf 2 of 27
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4. Which of the following accurately represents reverse B
typing?
A. Reverse typing is performed using known reagent
antisera to detect ABO antigens on the patient's red
blood cells.
B. Reverse typing is performed using known reagent
red blood cells to detect ABO antibodies in the pa-
tient's serum or plasma.
C. Reverse typing is performed using known reagent
screening cells to detect clinically significant antibod-
ies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent
antisera to phenotype donor red blood cells for clini-
cally significant antigens.
5. A former patient had an anti-E four years ago, but her B
antibody panel is now negative. Since she now needs
blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units.
6. What is the maximum interval during which a recipient B
sample may be used for compatibility testing if the
patient has recently been transfused or was pregnant
within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks
7. A patient transfused with two units of packed cells D
spiked a fever of 99.5oF and complained of chills
five days after transfusion. The direct antiglobulin test
(DAT) was positive with anti-IgG, but negative with
Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf
, Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf 3 of 27
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anti-C3d. Compatibility testing was performed on the
pre- and post-transfusion specimens. The post-trans-
fusion specimen was incompatible with one of the
donor units transfused. An antibody screen was done
on both the pre- and post-transfusion specimens. An
antibody was detected in the post-transfusion speci-
men only and identified by panel studies as anti-Jka.
This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction
8. You perform a gel ABO/Rh test on a 45-year-old male D
patient and you obtain the following results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS
9. When performing an anti-human globulin (AHG) test, D
it is important to completely wash the red cells be-
cause:
A. Washing eliminates concentrations of unbound
antigens.
B. Washing prevents elution of cell-bound antibody.
C. Washing promotes false positive effects of
Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf Blood Bank - Serologic and Molecular Testing.pdf
