UAMS HEME LAB PRACTICAL 3
CERTIFICATION EVALUATION SET
COMPLETE QUESTIONS AND
ANSWERS
◉ Non-Megaloblastic Answer: Macrocytic Anemia • Increase in
membrane lipids • MCV <110 fL; round macrocytes • Polychromasia
due to reticulocytosis
◉ round macrocytes Answer: Non-Megaloblastic
◉ • Due to a slowdown in DNA synthesis in developing cells Answer:
Megaloblastic Anemia
◉ Megaloblastic Anemia Defect is in Answer: DNA replication from a
depletion of thymidine triphosphate - An immediate DNA precursor
◉ B12 Answer: cyanocobalamin
◉ B12 comes from Answer: Meat, eggs, dairy products and liver
◉ B12 Intestinal absorption by glycoprotein Answer: called Intrinsic
Factor - IF is produced by parietal cells of the stomach IF-B12
complex attaches to receptors on ileac mucosal cells for absorption
,◉ B12 and folate are the Answer: required cofactors in the synthesis
of thymidine triphosphate and subsequently
◉ Metabolism of B,, and Folate After absorption Answer: vitamin is
released and attaches to transcobalamin II Complex goes to either •
Bone marrow • Liver for storage
◉ Vitamin B12 (Cobalamin) Deficiency Results in Answer: defective
DNA synthesis
◉ Vitamin B12 Deficiency Cause is Answer: almost always due to
absorption problem
◉ Lack of intrinsic factor Answer: Pernicious Anemia
◉ Diphyllobothium latum fish tapeworm Answer: Vitamin B12
Deficiency Cause
◉ Vitamin B12 Deficiency Diagnose with Answer: vitamin assay
◉ PA is one type of Answer: vitamin B,2 deficiency
,◉ B12 is one type of Answer: megaloblastic anemia
◉ Vitamin B,12 cannot be absorbed Answer: PA is due to lack of
intrinsic factor
◉ Pernicious Anemia Caused by Answer: autoimmune disorder with
autoantibodies produced by parietal cells
◉ Pernicious Anemia Treat with Answer: injections of vitamin B,2
Cannot use oral B12 - Life time treatment is required
◉ Other Causes of Megaloblastic Anemia Answer: Chemotherapy
and other drugs Myelodysplasia or premalignant "megaloblastoid"
disorders
◉ Specific Tests for PNH Answer: Sucrose Hemolysis and Ham Test
◉ Specific Tests for • For all Hemolytic Anemias Blood Smear
Answer: Hemolytic RBC fragments Spherocytes Polychromasia
Agglutination
◉ Normocytic, normochromic Answer: Hemolytic anemia • Red cells
have a shortened life span • Destroyed before 120 days
, ◉ Specific Tests for Autoimmune Hemolytic Anemia Answer: DAT
◉ Specific Tests for Hemoglobinopathies Answer: Hemoglobin
Electrophoresis
◉ Specific Tests for G6PD Deficiency Answer: Heinz bodies; G6PD
assay
◉ Specific Tests for Hereditary Spherocytosis and Elliptocytosis
Answer: Osmotic Fragility
◉ Specific Tests for G6PD Deficiency Answer: Heinz bodies; G6PD
assay
◉ Abnormal Tests in Intravascular Hemolysis Answer: • ↑ urine
hemoglobin • ↑ urine hemosiderin
◉ • ↑ urine hemoglobin • ↑ urine hemosiderin Answer: Intravascular
Hemolysis
◉ Abnormal Tests for Hemolytic Anemia Answer: •↑ unconjugated
bilirubin • ↑ LD
◉ • ⬇️Haptoglobin Answer: Hemolytic Anemia
CERTIFICATION EVALUATION SET
COMPLETE QUESTIONS AND
ANSWERS
◉ Non-Megaloblastic Answer: Macrocytic Anemia • Increase in
membrane lipids • MCV <110 fL; round macrocytes • Polychromasia
due to reticulocytosis
◉ round macrocytes Answer: Non-Megaloblastic
◉ • Due to a slowdown in DNA synthesis in developing cells Answer:
Megaloblastic Anemia
◉ Megaloblastic Anemia Defect is in Answer: DNA replication from a
depletion of thymidine triphosphate - An immediate DNA precursor
◉ B12 Answer: cyanocobalamin
◉ B12 comes from Answer: Meat, eggs, dairy products and liver
◉ B12 Intestinal absorption by glycoprotein Answer: called Intrinsic
Factor - IF is produced by parietal cells of the stomach IF-B12
complex attaches to receptors on ileac mucosal cells for absorption
,◉ B12 and folate are the Answer: required cofactors in the synthesis
of thymidine triphosphate and subsequently
◉ Metabolism of B,, and Folate After absorption Answer: vitamin is
released and attaches to transcobalamin II Complex goes to either •
Bone marrow • Liver for storage
◉ Vitamin B12 (Cobalamin) Deficiency Results in Answer: defective
DNA synthesis
◉ Vitamin B12 Deficiency Cause is Answer: almost always due to
absorption problem
◉ Lack of intrinsic factor Answer: Pernicious Anemia
◉ Diphyllobothium latum fish tapeworm Answer: Vitamin B12
Deficiency Cause
◉ Vitamin B12 Deficiency Diagnose with Answer: vitamin assay
◉ PA is one type of Answer: vitamin B,2 deficiency
,◉ B12 is one type of Answer: megaloblastic anemia
◉ Vitamin B,12 cannot be absorbed Answer: PA is due to lack of
intrinsic factor
◉ Pernicious Anemia Caused by Answer: autoimmune disorder with
autoantibodies produced by parietal cells
◉ Pernicious Anemia Treat with Answer: injections of vitamin B,2
Cannot use oral B12 - Life time treatment is required
◉ Other Causes of Megaloblastic Anemia Answer: Chemotherapy
and other drugs Myelodysplasia or premalignant "megaloblastoid"
disorders
◉ Specific Tests for PNH Answer: Sucrose Hemolysis and Ham Test
◉ Specific Tests for • For all Hemolytic Anemias Blood Smear
Answer: Hemolytic RBC fragments Spherocytes Polychromasia
Agglutination
◉ Normocytic, normochromic Answer: Hemolytic anemia • Red cells
have a shortened life span • Destroyed before 120 days
, ◉ Specific Tests for Autoimmune Hemolytic Anemia Answer: DAT
◉ Specific Tests for Hemoglobinopathies Answer: Hemoglobin
Electrophoresis
◉ Specific Tests for G6PD Deficiency Answer: Heinz bodies; G6PD
assay
◉ Specific Tests for Hereditary Spherocytosis and Elliptocytosis
Answer: Osmotic Fragility
◉ Specific Tests for G6PD Deficiency Answer: Heinz bodies; G6PD
assay
◉ Abnormal Tests in Intravascular Hemolysis Answer: • ↑ urine
hemoglobin • ↑ urine hemosiderin
◉ • ↑ urine hemoglobin • ↑ urine hemosiderin Answer: Intravascular
Hemolysis
◉ Abnormal Tests for Hemolytic Anemia Answer: •↑ unconjugated
bilirubin • ↑ LD
◉ • ⬇️Haptoglobin Answer: Hemolytic Anemia
