2026 | Practice Questions,
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Updated 2026 Questions and Answers
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Rationales
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,EDTA - Lavender tube -prevent clotting by chelating Ca2+
-should be at least half full
Heparin (green top) -prevent clotting by neutralizing thrombin
-don't use for diffs (has blue background)
-used for ABGs, plasma hgb, chemistries
Sodium citrate (light blue) top -binds calcium
-preserve labile clotting factors
-9:1
-most coag tests
Sodium fluoride (gray top) -inhibits glycolysis
-preserve glucose 24hr
- glucose, lactic acid, blood alcohol
Blood culture bottles Drawn first
Contain SPS
Order of draw for venipuncture 1. Yellow (Blood Culture)
2. Coagulation (light blue)
3. Serum (red, gold, speckled)
4. Heparin (green)
5. EDTA (lavender)
6. Glycolytic inhibitor (gray)
Order of Draw for Capillary Specimens 1. Blood gases
2. EDTA (lavender)
3. Heparin (green)
4. Other additives
5. Serum tubes
Chilled tests ACTH, acetone, ammonia, gastrin, glucagon, lactic acid, pyruvate, parathyroid
hormone (PTH), renin
Protect from light bilirubin, many vitamins, carotene, folate, protoporphyrin
1(2s) Rule 1 ctl > +/- 2s from mean. Warning
1 3s rule 1 ctl > +/- 3 s from mean. Reject
2 (2s) rule Two consecutive control values for the same level fall outside of +/- 2 SD in the
same direction
Reject
R (4s) rule 2 consecutive controls differ by >4s. Reject
, 4 (1s) rule 4 consecutive control values lie in the same direction from the mean and exceed the
mean by more than 1 SD.
Reject
10x rule 10 consecutive values fall on same side of mean
Reject
Sensitivity TP / (TP + FN) x 100
TP = true pos
FN= false neg
Specificity TN / (TN + FP) x 100
Positive Predictive Value (PPV) TP/(TP+FP) x100
Negative predictive value NPV = TN / (TN + FN) x 100
Delta checks quality control that allows for detection of clinically significant changes in laboratory
results
correlation study study that determines the relationship between 2 variables
Deci 10^-1
centi 10^-2
milli 10^-3
micro 10^-6
nano 10^-9
pico 10^-12
femto 10^-15