biol 2051 lab final review (lab quizzes) Questions with
100% Verified Answers Latest Update
Question: true or false: a layer of slime formed at the surface of the N-free broth inoculated
with soil could be the polysaccharide from Azotobacter.
Answer:
true
Question: true or false: a N-free plate was used to isolate nitrogen-fixing bacteria from the
N-free broth culture.
Answer:
true
Question: which stains do you preform to determine if you've isolated Azotobacter?
Answer:
gram stain and capsule stain
Question: you conducted the IMViC series on an unknown bacterial culture and the results
show that the indole test is negative, methyl red is positive and the citrate test is negative.
is the organism e. coli?
Answer:
no
Question: true or false: if you saw beta hemolysis on your agar plate, then you have strep
throat.
Answer:
false
Question: what color change is observed from the growth of lactobacilli in synder's test
agar?
Answer:
green to yellow
Question: if a doctor suspects a patient is infected with s. aureus, which pieces of media can
be he inoculate on order to give him relevant date and help him make a diagnosis?
Answer:
tube of plasma, MSA plate
Question: true or false: s. epidermidis tests positive for coagulase production which can be
, seen by the zone of hydrolysis on a blood agar plate.
Answer:
false
Question: true or false: azotobacter is a free-living bacteria capable of fixing nitrogen
aerobically.
Answer:
true
Question: true or false: the only reason why we use a loose cotton plug for the tubes is to
allow oxygen to enter the tubes. the organisms we use are aerobic.
Answer:
false
Question: true or false: when you remove the N-free broth culture from the room temp
shelf, shake it well.
Answer:
false
Question: which medium is used to test for the presence of butane-diol fermentation?
Answer:
MRVP broth that contains glucose
Question: true or false: to identify a coliform isolated on an EMB plate from the completed
test of the MPN technique, you will inoculate a set of IMViC media.
Answer:
true
Question: what is done to complete the IMViC series of tests?
Answer:
to an MRVP broth culture add methyl red and look for red color
Question: true or false: for the indole test, you add a dropper full of Kovac's reagent and mix
the tube vigorously. a red color throughout the tube indicates a positive result.
Answer:
false
Question: true or false: non-lactose fermenting bacteria can be distinguished from lactose
100% Verified Answers Latest Update
Question: true or false: a layer of slime formed at the surface of the N-free broth inoculated
with soil could be the polysaccharide from Azotobacter.
Answer:
true
Question: true or false: a N-free plate was used to isolate nitrogen-fixing bacteria from the
N-free broth culture.
Answer:
true
Question: which stains do you preform to determine if you've isolated Azotobacter?
Answer:
gram stain and capsule stain
Question: you conducted the IMViC series on an unknown bacterial culture and the results
show that the indole test is negative, methyl red is positive and the citrate test is negative.
is the organism e. coli?
Answer:
no
Question: true or false: if you saw beta hemolysis on your agar plate, then you have strep
throat.
Answer:
false
Question: what color change is observed from the growth of lactobacilli in synder's test
agar?
Answer:
green to yellow
Question: if a doctor suspects a patient is infected with s. aureus, which pieces of media can
be he inoculate on order to give him relevant date and help him make a diagnosis?
Answer:
tube of plasma, MSA plate
Question: true or false: s. epidermidis tests positive for coagulase production which can be
, seen by the zone of hydrolysis on a blood agar plate.
Answer:
false
Question: true or false: azotobacter is a free-living bacteria capable of fixing nitrogen
aerobically.
Answer:
true
Question: true or false: the only reason why we use a loose cotton plug for the tubes is to
allow oxygen to enter the tubes. the organisms we use are aerobic.
Answer:
false
Question: true or false: when you remove the N-free broth culture from the room temp
shelf, shake it well.
Answer:
false
Question: which medium is used to test for the presence of butane-diol fermentation?
Answer:
MRVP broth that contains glucose
Question: true or false: to identify a coliform isolated on an EMB plate from the completed
test of the MPN technique, you will inoculate a set of IMViC media.
Answer:
true
Question: what is done to complete the IMViC series of tests?
Answer:
to an MRVP broth culture add methyl red and look for red color
Question: true or false: for the indole test, you add a dropper full of Kovac's reagent and mix
the tube vigorously. a red color throughout the tube indicates a positive result.
Answer:
false
Question: true or false: non-lactose fermenting bacteria can be distinguished from lactose
