BIOL 2051 Lab Midterm Theory Questions with 100%
Verified Answers Latest Update
Question: Microorganisms growing on a solid culture medium will form ____________ if the
cells used to inoculate the plate are separated from each other on the culture medium
Answer:
colonies
Question: T/F a single cell will give rise to multiple colonies
Answer:
F
Question: colony morphology
Answer:
the properties and characteristics of colonies such as shape, margin, and the elevation
Question: T/F genetically different organisms can NOT share the same colony morphology
Answer:
F
Question: if more than one type of colony morphology is present on a plate, this may
indicate the presence of a ______________
Answer:
contaminant
Question: Molds are made of branching, intertwined, threadlike ________ forming a network
called ____________.
Answer:
hyphae; mycelium
Question: T/F Mycelium can grow far beyond the colony observed on the agar surface
Answer:
T
Question: Pathogenic
Answer:
Causing or able to cause disease
,Question: Hyphae
Answer:
cellular and fungi filaments that look like branches as part of normal growth by bacteria like
Streptomyces
Question: The observable mold colony is most likely the __________________ releasing spores
Answer:
aerial hyphae
Question: Mycelium
Answer:
network of hyphae formed by the growth of species of bacteria like Streptomyces and fungi
Question: the colony morphology is dependent on the __________ on which its is growing
Answer:
medium
Question: medium
Answer:
a solution that contains nutrients, such as carbon and nitrogen, required for cells to grow;
agar can be added to be made solid
Question: TSA plates
Answer:
trypticase soy agar plates
Question: Whole Colony Shape
Answer:
round, irregular, rhizoid
Question: Margin Shape
Answer:
smooth, lobate, filamentous
Question: Elevation
Answer:
convex, umbonate (button-like), flat
,Question: Optical properties
Answer:
opaque, semi-tranleucent, translucent
Question: Surface Characteristics
Answer:
dull, shiny, wrinkled, concentric, pigmentation
Question: Purpose of Exp 1
Answer:
this experiment will identify source of contaminants in the lab and the necessity of using
proper aseptic technique to prevent pure bacterial cultures from becoming contaminated
with undesired organisms from the environment
Question: the ________ of microorganisms are pathogenic
Answer:
minority
Question: can nonpathogenic microorganisms cause disease?
Answer:
yes, if someone is sick or injured
Question: Nosocomial
Answer:
an infection acquired in a hospital
Question: Ignaz Semmelweis
Answer:
Identified cause of childbed fever. Emphasized doctor cleanliness.
Question: handwashing prevents the spread of disease by reducing the possibility of
transmitting ___________
Answer:
an infectious dose
Question: infectious dose
, Answer:
the number of microbes necessary to establish an infection
Question: Florence Nightingale
Answer:
brought the practice of handwashing and aseptic technique to the field of nursing
Question: Normal or resident microbiota (flora)
Answer:
establish themselves on or in the body, but do not produce disease under normal conditions
- can survive and multiply on the body
- corynebacterium
- propionibacterium
- malassezia
- S. aureus, S. epidermidis
Question: T/F an organism can be harmless on skin, but cause disease if introduced into the
bloodstream
Answer:
T
Question: which part of the skin are normal skin flora usually associated with and why?
Answer:
the apocrine and sebaceous glands; provide moisture and nutrients for microbial growth
Question: What does hand washing do to normal skin flora?
Answer:
sloughs off the dead outermost layers of the skin and may expose normal flora after
vigorous washing, many microorganisms may still be on the skin and the species present
may change there may be even more after handwashing
Question: opportunistic pathogens
Answer:
normal microbiota that cause disease under certain circumstances
Question: Transient microbiota (flora)
Answer:
may be present on the body temporarily but do not become firmly entrenched; come into
contact with skin constantly, but most organisms are unable to multiply there and will die
Verified Answers Latest Update
Question: Microorganisms growing on a solid culture medium will form ____________ if the
cells used to inoculate the plate are separated from each other on the culture medium
Answer:
colonies
Question: T/F a single cell will give rise to multiple colonies
Answer:
F
Question: colony morphology
Answer:
the properties and characteristics of colonies such as shape, margin, and the elevation
Question: T/F genetically different organisms can NOT share the same colony morphology
Answer:
F
Question: if more than one type of colony morphology is present on a plate, this may
indicate the presence of a ______________
Answer:
contaminant
Question: Molds are made of branching, intertwined, threadlike ________ forming a network
called ____________.
Answer:
hyphae; mycelium
Question: T/F Mycelium can grow far beyond the colony observed on the agar surface
Answer:
T
Question: Pathogenic
Answer:
Causing or able to cause disease
,Question: Hyphae
Answer:
cellular and fungi filaments that look like branches as part of normal growth by bacteria like
Streptomyces
Question: The observable mold colony is most likely the __________________ releasing spores
Answer:
aerial hyphae
Question: Mycelium
Answer:
network of hyphae formed by the growth of species of bacteria like Streptomyces and fungi
Question: the colony morphology is dependent on the __________ on which its is growing
Answer:
medium
Question: medium
Answer:
a solution that contains nutrients, such as carbon and nitrogen, required for cells to grow;
agar can be added to be made solid
Question: TSA plates
Answer:
trypticase soy agar plates
Question: Whole Colony Shape
Answer:
round, irregular, rhizoid
Question: Margin Shape
Answer:
smooth, lobate, filamentous
Question: Elevation
Answer:
convex, umbonate (button-like), flat
,Question: Optical properties
Answer:
opaque, semi-tranleucent, translucent
Question: Surface Characteristics
Answer:
dull, shiny, wrinkled, concentric, pigmentation
Question: Purpose of Exp 1
Answer:
this experiment will identify source of contaminants in the lab and the necessity of using
proper aseptic technique to prevent pure bacterial cultures from becoming contaminated
with undesired organisms from the environment
Question: the ________ of microorganisms are pathogenic
Answer:
minority
Question: can nonpathogenic microorganisms cause disease?
Answer:
yes, if someone is sick or injured
Question: Nosocomial
Answer:
an infection acquired in a hospital
Question: Ignaz Semmelweis
Answer:
Identified cause of childbed fever. Emphasized doctor cleanliness.
Question: handwashing prevents the spread of disease by reducing the possibility of
transmitting ___________
Answer:
an infectious dose
Question: infectious dose
, Answer:
the number of microbes necessary to establish an infection
Question: Florence Nightingale
Answer:
brought the practice of handwashing and aseptic technique to the field of nursing
Question: Normal or resident microbiota (flora)
Answer:
establish themselves on or in the body, but do not produce disease under normal conditions
- can survive and multiply on the body
- corynebacterium
- propionibacterium
- malassezia
- S. aureus, S. epidermidis
Question: T/F an organism can be harmless on skin, but cause disease if introduced into the
bloodstream
Answer:
T
Question: which part of the skin are normal skin flora usually associated with and why?
Answer:
the apocrine and sebaceous glands; provide moisture and nutrients for microbial growth
Question: What does hand washing do to normal skin flora?
Answer:
sloughs off the dead outermost layers of the skin and may expose normal flora after
vigorous washing, many microorganisms may still be on the skin and the species present
may change there may be even more after handwashing
Question: opportunistic pathogens
Answer:
normal microbiota that cause disease under certain circumstances
Question: Transient microbiota (flora)
Answer:
may be present on the body temporarily but do not become firmly entrenched; come into
contact with skin constantly, but most organisms are unable to multiply there and will die
