BIOL 2051 lab midterm Questions with 100% Verified
Answers Latest Update
Question: Lab technique 1
Answer:
Media prep and sterilization
Question: lab organisms can be grown in
Answer:
lab or culture medium
Question:
Answer:
Question: culture medium
Answer:
contains nutrients, such as carbon and nitrogen, required for cells to grow
Question: complex medium
Answer:
composed of digests of chemically undefined substances such as yeast and meat extracts
Question: defined medium
Answer:
one for which a precise chemical composition is known
Question: Nutrient Agar and Trypticase Soy Agar
Answer:
complex, general-purpose media for the isolation and cultivation of a variety of
microorganisms
Question: Sabourand dextrose agar
Answer:
complex medium for growing fungi or yeast
Question: Culture media can be
, Answer:
liquid or solid
Question: Liquid medium is called
Answer:
broth
Question: Agar is
Answer:
extracted from Rhodophyta (red algae)
useful as solid media because after being heated it solidies at 40-42C
not broken down or digested by microbes
Question: Plates
Answer:
solid media in petri dishes
Question: Deeps
Answer:
test tubes containing solid medium which has been allowed to solidify in an upright position
and usually used for anaerobic growth
Question: slants
Answer:
test tubes containing solid medium that has been allowed to solidify at an angle and the
surface of the agar is used for maintenance of stock cultures of microorganisms
,Question: media can be
Answer:
selective, differential, both, or neither
Question: selective medium
Answer:
a nutrient medium designed to favor the growth of certain microbes and inhibit undesirable
competitors
PEA (phenyl alcohol agar)
Question: differential medium
Answer:
a medium which provides a visible indication of a physiological characteristic of a
microorganism
ex: carbohydrate fermentation or enzyme production
Question: MSA (Mannitol salt agar)
Answer:
both selective and differential
selects for growth of salt-tolerant organisms and differentiates mannitol fermenters'
Question: EMB (eosin methylene blue agar)
Answer:
both selective and differential
selects for gram negative bacteria and differentiates for lactose fermenters
Question: sterilization
Answer:
the killing or removal of all living organisms and their viruses from a growth medium
Question: autoclave
Answer:
a sealed device that allows the entrance of steam under pressure
, moist heat facilitates killing all microorganisms including endospores
heated to 121C at 15 pounds per square inch (psi) for 15min to 1hr
Question: dry heat sterilization
Answer:
kills by oxidation effects
Question: flaming
Answer:
method of dry heat sterilization
when inoculating loops and needles are held in the flame of the bunsen burner until glowing
red hot
Question: hot-air sterilization
Answer:
items to be sterilized like glassware are placed in an oven and heated to 170F for 2 hours
Question: filtration
Answer:
sterilizes heat sensitive liquids or gases
Question: gas chemo sterilizers
Answer:
sterilizes heat sensitive objects such as plastic petri dishes
widely used gas is ethylene oxide
-ethylene oxide also used to sterilize paper, leather, wood, and inside spacecrafts
termed cold-sterilization because no heat is used
Question: electromagnetic raditation
Answer:
also used to sterilize substances
microwaves, ultraviolet radiation, etc
Question: Ultraviolet (UV) radiation
Answer:
causes damage to DNA leading to death of exposed organisms
useful to sterilize exposed surfaces
Answers Latest Update
Question: Lab technique 1
Answer:
Media prep and sterilization
Question: lab organisms can be grown in
Answer:
lab or culture medium
Question:
Answer:
Question: culture medium
Answer:
contains nutrients, such as carbon and nitrogen, required for cells to grow
Question: complex medium
Answer:
composed of digests of chemically undefined substances such as yeast and meat extracts
Question: defined medium
Answer:
one for which a precise chemical composition is known
Question: Nutrient Agar and Trypticase Soy Agar
Answer:
complex, general-purpose media for the isolation and cultivation of a variety of
microorganisms
Question: Sabourand dextrose agar
Answer:
complex medium for growing fungi or yeast
Question: Culture media can be
, Answer:
liquid or solid
Question: Liquid medium is called
Answer:
broth
Question: Agar is
Answer:
extracted from Rhodophyta (red algae)
useful as solid media because after being heated it solidies at 40-42C
not broken down or digested by microbes
Question: Plates
Answer:
solid media in petri dishes
Question: Deeps
Answer:
test tubes containing solid medium which has been allowed to solidify in an upright position
and usually used for anaerobic growth
Question: slants
Answer:
test tubes containing solid medium that has been allowed to solidify at an angle and the
surface of the agar is used for maintenance of stock cultures of microorganisms
,Question: media can be
Answer:
selective, differential, both, or neither
Question: selective medium
Answer:
a nutrient medium designed to favor the growth of certain microbes and inhibit undesirable
competitors
PEA (phenyl alcohol agar)
Question: differential medium
Answer:
a medium which provides a visible indication of a physiological characteristic of a
microorganism
ex: carbohydrate fermentation or enzyme production
Question: MSA (Mannitol salt agar)
Answer:
both selective and differential
selects for growth of salt-tolerant organisms and differentiates mannitol fermenters'
Question: EMB (eosin methylene blue agar)
Answer:
both selective and differential
selects for gram negative bacteria and differentiates for lactose fermenters
Question: sterilization
Answer:
the killing or removal of all living organisms and their viruses from a growth medium
Question: autoclave
Answer:
a sealed device that allows the entrance of steam under pressure
, moist heat facilitates killing all microorganisms including endospores
heated to 121C at 15 pounds per square inch (psi) for 15min to 1hr
Question: dry heat sterilization
Answer:
kills by oxidation effects
Question: flaming
Answer:
method of dry heat sterilization
when inoculating loops and needles are held in the flame of the bunsen burner until glowing
red hot
Question: hot-air sterilization
Answer:
items to be sterilized like glassware are placed in an oven and heated to 170F for 2 hours
Question: filtration
Answer:
sterilizes heat sensitive liquids or gases
Question: gas chemo sterilizers
Answer:
sterilizes heat sensitive objects such as plastic petri dishes
widely used gas is ethylene oxide
-ethylene oxide also used to sterilize paper, leather, wood, and inside spacecrafts
termed cold-sterilization because no heat is used
Question: electromagnetic raditation
Answer:
also used to sterilize substances
microwaves, ultraviolet radiation, etc
Question: Ultraviolet (UV) radiation
Answer:
causes damage to DNA leading to death of exposed organisms
useful to sterilize exposed surfaces
