BIOL 2051 Lab Midterm Quizzes Questions with 100%
Verified Answers Latest Update
Question: True
Answer:
T/F Never open a petri dish which has something furry growing in it, obvious fungal growth
Question: False
Answer:
T/F All microorganisms used in the BIOL 2051 are BioSafety Level IV pathogens.
Question: When you enter lab, which of the following are done?
Answer:
place your cell phone in your backpack for safe storage during lab
Question: let others nearby and the TA know and use a disinfectant like vesphene and paper
towels to clean up the spill
Answer:
If you spill live culture on the bench top,
Question: Which of the following must you wear during lab?
Answer:
gloves
Question: False
Answer:
T/F Cell phones are allowed on the bench tops as long as you don't touch them during
experiments.
Question: False
Answer:
T/F Broken glass and slides are discarded in the red plastic biohazard box.
Question: False
Answer:
T/F Gloves can be worn during the entire lab, even when touching "clean" surfaces like the
door knob to the incubator room or the faucet.
,Question: Before you leave the lab, which of the following are done?
Answer:
Wash your hands, Remove labels from glass tubes you place in the discard tubs under the
cart, Discard used plates in the white biohazard trash can, Return materials neatly to the
storage drawers, Turn off Bunsen burners, and Wash bench top with disinfectant
Question: Before doing an experiment, which of the following are done?
Answer:
Tie back loose hair, Wash bench top with disinfectant, Put on gloves, and Put on safety
glasses
Question: What is the instrument (or tool) that uses high heat and pressure to sterilize
glass, or liquid media?
Answer:
Autoclave
Question: What is true about a simple stain?
Answer:
Is a way to provide contrast to a smear, Is a water or alcohol based solution, stains the
entire cell, contains only a single basic dye
Question: False
Answer:
T/F Nosocomial diseases - a term to describe diseases that are acquired through one's nasal
cavity.
Question: What is the prefix used to describe a chain of bacterial cells?
Answer:
Strepto
Question: Transient
Answer:
Hand washing with soap typically removes ________________ flora from your hands.
Question: False
Answer:
T/F Plastic agar plate can be sterilized by autoclaving.
, Question: colony
Answer:
A population of cells which arise from a single cell growing on a solid medium is a
Question: Which of the following is the best explanation for using stains on smears. Select
one:
a. Staining makes the cells visible by coloring them darker than the light background.
b. Staining makes the smears smell better.
c. Staining allows you to better determine the colony morphology.
d. Staining adds liquid to the cells to make them bigger and easier to see.
Answer:
A. staining makes the cells visible by coloring them darker than the light background
Question: Which of following is used to determine colony morphology of a microorganism?
Select one or more:
a. a colony
b. a broth culture
c. a microscope
d. your eye
Answer:
a. a colony
d. your eye
Question: Why should you always keep your broth tube at an angle? Select one:
a. This prevents the culture in the tube from contaminating the air.
b. This prevents the flame from burning the culture.
c. None of the above.
d. This prevents contaminants from entering the tube.
Answer:
d. This prevents contaminants from entering the tube
Question: False
Answer:
T/F After inoculating quadrant 1 of the plate, flame the loop, wait for the loop to cool, obtain
more bacteria from the broth, and then inoculate quadrant 2.
Question: d. all of the above
Answer:
A smear can be made from Select one:
a. Bacteria growing on a solid medium
b. A broth culture
Verified Answers Latest Update
Question: True
Answer:
T/F Never open a petri dish which has something furry growing in it, obvious fungal growth
Question: False
Answer:
T/F All microorganisms used in the BIOL 2051 are BioSafety Level IV pathogens.
Question: When you enter lab, which of the following are done?
Answer:
place your cell phone in your backpack for safe storage during lab
Question: let others nearby and the TA know and use a disinfectant like vesphene and paper
towels to clean up the spill
Answer:
If you spill live culture on the bench top,
Question: Which of the following must you wear during lab?
Answer:
gloves
Question: False
Answer:
T/F Cell phones are allowed on the bench tops as long as you don't touch them during
experiments.
Question: False
Answer:
T/F Broken glass and slides are discarded in the red plastic biohazard box.
Question: False
Answer:
T/F Gloves can be worn during the entire lab, even when touching "clean" surfaces like the
door knob to the incubator room or the faucet.
,Question: Before you leave the lab, which of the following are done?
Answer:
Wash your hands, Remove labels from glass tubes you place in the discard tubs under the
cart, Discard used plates in the white biohazard trash can, Return materials neatly to the
storage drawers, Turn off Bunsen burners, and Wash bench top with disinfectant
Question: Before doing an experiment, which of the following are done?
Answer:
Tie back loose hair, Wash bench top with disinfectant, Put on gloves, and Put on safety
glasses
Question: What is the instrument (or tool) that uses high heat and pressure to sterilize
glass, or liquid media?
Answer:
Autoclave
Question: What is true about a simple stain?
Answer:
Is a way to provide contrast to a smear, Is a water or alcohol based solution, stains the
entire cell, contains only a single basic dye
Question: False
Answer:
T/F Nosocomial diseases - a term to describe diseases that are acquired through one's nasal
cavity.
Question: What is the prefix used to describe a chain of bacterial cells?
Answer:
Strepto
Question: Transient
Answer:
Hand washing with soap typically removes ________________ flora from your hands.
Question: False
Answer:
T/F Plastic agar plate can be sterilized by autoclaving.
, Question: colony
Answer:
A population of cells which arise from a single cell growing on a solid medium is a
Question: Which of the following is the best explanation for using stains on smears. Select
one:
a. Staining makes the cells visible by coloring them darker than the light background.
b. Staining makes the smears smell better.
c. Staining allows you to better determine the colony morphology.
d. Staining adds liquid to the cells to make them bigger and easier to see.
Answer:
A. staining makes the cells visible by coloring them darker than the light background
Question: Which of following is used to determine colony morphology of a microorganism?
Select one or more:
a. a colony
b. a broth culture
c. a microscope
d. your eye
Answer:
a. a colony
d. your eye
Question: Why should you always keep your broth tube at an angle? Select one:
a. This prevents the culture in the tube from contaminating the air.
b. This prevents the flame from burning the culture.
c. None of the above.
d. This prevents contaminants from entering the tube.
Answer:
d. This prevents contaminants from entering the tube
Question: False
Answer:
T/F After inoculating quadrant 1 of the plate, flame the loop, wait for the loop to cool, obtain
more bacteria from the broth, and then inoculate quadrant 2.
Question: d. all of the above
Answer:
A smear can be made from Select one:
a. Bacteria growing on a solid medium
b. A broth culture
