BIOL 2051 LAB FINAL Exam Questions with 100%
Verified Answers Latest Update
Question: enzymes
Answer:
proteins that serve as biological catalysts
Question: substrate
Answer:
substance that reacts with an enzyme
Question: generally, how many substrates may an enzyme react with?
Answer:
one
Question: extracellular enzymes
Answer:
enzymes that act outside of the cell in which they are produced
Question: Generally, how are extracellular enzymes useful?
Answer:
degrade large molecules into units that are small enough to be transported by proteins
across the cell membrane and into the cell where they may be used
Question: endoenzymes
Answer:
act inside of the cell to synthesize molecules that are needed by the cell OR degrade
molecules to simpler molecules, releasing energy
Question: inducible
Answer:
type of enzyme that is only produced when a substrate is available
Question: constitutive
Answer:
type of enzyme that are essential to the cell and produced at all times
Question: morphology
, Answer:
cell size, shape, and arrangement of a bacterium
Question: how an organism functions in an environment; its metabolic capabilities..
enzymes produced and biochemical reactions
Answer:
physiology
Question: What two enzymes are made by organisms that produce energy using aerobic
respiration?
Answer:
oxidase and catalase
Question: molecular oxygen
Answer:
in aerobic respiration, what serves as the final electron acceptor of the electron transport
chain
Question: oxygen accepts an electron from what protein in the electron transport chain
during aerobic respiration?
Answer:
cytochrome oxidase
Question: What is tested for in an oxidase assay?
Answer:
cytochrome c oxidase
Question: Many oxidase negative organisms are what in terms of oxygen use?
Answer:
facultative aerobes
Question: What are produced by the reduction of oxygen?
Answer:
toxic byproducts
Question: An organism's catalase production gives you information about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
,C) the presence of nitrate reductase in the cell
D) fermentation properties
Answer:
B
Question: true
Answer:
T/F a microscope slide is a convenient surface to perform a catalase test
Question: Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation
Answer:
B
Question: true
Answer:
T/F Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome
oxidase for aerobic respiration
Question: T/F Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic
respiration
Answer:
true
Question: T/F dark-dier mutant of C. reinhardtii can grow on minimal media
Answer:
true
Question: T/F dark-dier mutant of C. reinhardtii is an anoxygenic phototroph
Answer:
false
Question: Which of the following describes how you will inoculate an HA plate to test the
effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on the other
side
, C) streak plate
D) pour plate
Answer:
B
Question: true
Answer:
T/F denitrification by soil microbes depletes the soil of nitrogen
Question: Which of the following are inoculated to test for nitrate reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
D) nitrite broth
Answer:
A
Question: false
Answer:
T/F To perform the catalase test, add a drop of water to the slide to mix with the bacteria
Question: How is aerobic respiration distinguished form anaerobic respiration?
Answer:
molecular oxygen serves as the electron acceptor
Question: T/F wild-type C. reinhardtii cannot use acetate in the dark for aerobic respiration
Answer:
false
Question: true
Answer:
T/F an organism is doing anaerobic respiration if it uses nitrate at the end of the electron
transport chain as the final electron acceptor
Question: false
Answer:
T/F the positive control tube for nitrate reduction is inoculated with both E. Coli and P.
denitrificans
Verified Answers Latest Update
Question: enzymes
Answer:
proteins that serve as biological catalysts
Question: substrate
Answer:
substance that reacts with an enzyme
Question: generally, how many substrates may an enzyme react with?
Answer:
one
Question: extracellular enzymes
Answer:
enzymes that act outside of the cell in which they are produced
Question: Generally, how are extracellular enzymes useful?
Answer:
degrade large molecules into units that are small enough to be transported by proteins
across the cell membrane and into the cell where they may be used
Question: endoenzymes
Answer:
act inside of the cell to synthesize molecules that are needed by the cell OR degrade
molecules to simpler molecules, releasing energy
Question: inducible
Answer:
type of enzyme that is only produced when a substrate is available
Question: constitutive
Answer:
type of enzyme that are essential to the cell and produced at all times
Question: morphology
, Answer:
cell size, shape, and arrangement of a bacterium
Question: how an organism functions in an environment; its metabolic capabilities..
enzymes produced and biochemical reactions
Answer:
physiology
Question: What two enzymes are made by organisms that produce energy using aerobic
respiration?
Answer:
oxidase and catalase
Question: molecular oxygen
Answer:
in aerobic respiration, what serves as the final electron acceptor of the electron transport
chain
Question: oxygen accepts an electron from what protein in the electron transport chain
during aerobic respiration?
Answer:
cytochrome oxidase
Question: What is tested for in an oxidase assay?
Answer:
cytochrome c oxidase
Question: Many oxidase negative organisms are what in terms of oxygen use?
Answer:
facultative aerobes
Question: What are produced by the reduction of oxygen?
Answer:
toxic byproducts
Question: An organism's catalase production gives you information about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
,C) the presence of nitrate reductase in the cell
D) fermentation properties
Answer:
B
Question: true
Answer:
T/F a microscope slide is a convenient surface to perform a catalase test
Question: Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation
Answer:
B
Question: true
Answer:
T/F Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome
oxidase for aerobic respiration
Question: T/F Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic
respiration
Answer:
true
Question: T/F dark-dier mutant of C. reinhardtii can grow on minimal media
Answer:
true
Question: T/F dark-dier mutant of C. reinhardtii is an anoxygenic phototroph
Answer:
false
Question: Which of the following describes how you will inoculate an HA plate to test the
effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on the other
side
, C) streak plate
D) pour plate
Answer:
B
Question: true
Answer:
T/F denitrification by soil microbes depletes the soil of nitrogen
Question: Which of the following are inoculated to test for nitrate reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
D) nitrite broth
Answer:
A
Question: false
Answer:
T/F To perform the catalase test, add a drop of water to the slide to mix with the bacteria
Question: How is aerobic respiration distinguished form anaerobic respiration?
Answer:
molecular oxygen serves as the electron acceptor
Question: T/F wild-type C. reinhardtii cannot use acetate in the dark for aerobic respiration
Answer:
false
Question: true
Answer:
T/F an organism is doing anaerobic respiration if it uses nitrate at the end of the electron
transport chain as the final electron acceptor
Question: false
Answer:
T/F the positive control tube for nitrate reduction is inoculated with both E. Coli and P.
denitrificans
