BIO 110 LAB QUIZ #2 QUESTIONS AND ANSWERS | COMPLETE BIOLOGY LAB
QUIZ 2 STUDY GUIDE 2026/2027
Enzyme - ANS ✔✔a catalyst; a protein that speeds up a chm rxn by lowering amount of energy it takes to
get the rxn going
What is the reactant that enzyme acts on? - ANS ✔✔substrate
What is the active site? - ANS ✔✔region on enzyme where substrate binds
Lock and Key - ANS ✔✔Shape of active site and shape of substrate fit together like a lock and key( or a
puzzle)
Enzyme Action (5) - ANS ✔✔1) substrate enters active site
2) held by weak interactions
3) active site can lower energy activity and speed up rxn
4) converted to products
5) products released
Enzymes are not what? - ANS ✔✔changed up or used up in a chm rxn
Are enzymes reusable? - ANS ✔✔YES
A cell has approximately how many different enzymes? - ANS ✔✔1000
What is a catalyst? - ANS ✔✔anything that speeds up a rxn; rxn would be so much slower without it;
without being used up in a rxn
buffer - ANS ✔✔substance added to solution to resist changes in pH of solution
temp and pH affect what? - ANS ✔✔enzyme activity
What is denaturation? - ANS ✔✔enzyme is unraveled and is destroyed since it doesn't have an active site
so they can't work anymore; losing its shape
Can denaturation reverse? - ANS ✔✔NO
What is the purpose of utilizing the Benedict's Test when examining the effect of concentration, temp,
and pH on enzyme activity? - ANS ✔✔To determine the presence of glucose or fructose which indicate
activity of the enzyme Invertase. sucrose does not react so if there is a rxn taking place noticed by color
change, the simple sugars are present.
How does enzyme concentration affect rxn? - ANS ✔✔As enzyme concentration goes up, enzyme activity
will increase because there are many more enzymes present to help break down the substrate; linear
relationship shown in graph
QUIZ 2 STUDY GUIDE 2026/2027
Enzyme - ANS ✔✔a catalyst; a protein that speeds up a chm rxn by lowering amount of energy it takes to
get the rxn going
What is the reactant that enzyme acts on? - ANS ✔✔substrate
What is the active site? - ANS ✔✔region on enzyme where substrate binds
Lock and Key - ANS ✔✔Shape of active site and shape of substrate fit together like a lock and key( or a
puzzle)
Enzyme Action (5) - ANS ✔✔1) substrate enters active site
2) held by weak interactions
3) active site can lower energy activity and speed up rxn
4) converted to products
5) products released
Enzymes are not what? - ANS ✔✔changed up or used up in a chm rxn
Are enzymes reusable? - ANS ✔✔YES
A cell has approximately how many different enzymes? - ANS ✔✔1000
What is a catalyst? - ANS ✔✔anything that speeds up a rxn; rxn would be so much slower without it;
without being used up in a rxn
buffer - ANS ✔✔substance added to solution to resist changes in pH of solution
temp and pH affect what? - ANS ✔✔enzyme activity
What is denaturation? - ANS ✔✔enzyme is unraveled and is destroyed since it doesn't have an active site
so they can't work anymore; losing its shape
Can denaturation reverse? - ANS ✔✔NO
What is the purpose of utilizing the Benedict's Test when examining the effect of concentration, temp,
and pH on enzyme activity? - ANS ✔✔To determine the presence of glucose or fructose which indicate
activity of the enzyme Invertase. sucrose does not react so if there is a rxn taking place noticed by color
change, the simple sugars are present.
How does enzyme concentration affect rxn? - ANS ✔✔As enzyme concentration goes up, enzyme activity
will increase because there are many more enzymes present to help break down the substrate; linear
relationship shown in graph
