BIOL 271 Module 3 (Latest Update 2026 /
2027) Microbiology Questions & Answers
{Grade A} 100% Correct - Portage Learning
What can electron microscopes see? - Correct answer Prokaryotic cells, viruses,
macromolecules and large atoms
List all of the 5 types of Light microscopes (teaching laboratory) - Correct answer 1.
Bright-field
2. Phase Contrast
3. Dark Field
4. Fluorescence
5. Confocal (laser scanning)
Bright-field: list the 3 uses - Correct answer 1. count microorganism or cells
2. view stained specimen
3. view live unstained specimen
Bright-field: list the feature(s) - Correct answer staining usually required
,Bright-field: list the types of image(s) - Correct answer bright background with clear
or colored specimen
Bright-field: specs for memorization - Correct answer halogen bulb > light from base
is reflected to sample
Condeser - Correct answer converges light beams into a focused area on the sample
iris diaphragm - Correct answer controls the amount of light that passes through the
sample and into the objective lens
objective lens - Correct answer lens closest to the sample; greatest amount of
magnification (more light = more magnification degree)
ocular lens - Correct answer eyepiece for the eye; (10x)
multicaptive - Correct answer when ocular and objective lens' are used together
, (10x + 40x = 400x magnification)
what is required before staining? - Correct answer heat or chemical methods
Phase Contrast: uses - Correct answer 1. view internal structures of live specimen
2. observe motility (cilia flagella)
specialized
Phase Contrast: features - Correct answer no staining necessary so we can view LIVE
specimens
Phase Contrast: images - Correct answer show light and dark areas of microbe
Phase Contrast: advantages - Correct answer over bright > able to visualize invisible
structures without staining.
2027) Microbiology Questions & Answers
{Grade A} 100% Correct - Portage Learning
What can electron microscopes see? - Correct answer Prokaryotic cells, viruses,
macromolecules and large atoms
List all of the 5 types of Light microscopes (teaching laboratory) - Correct answer 1.
Bright-field
2. Phase Contrast
3. Dark Field
4. Fluorescence
5. Confocal (laser scanning)
Bright-field: list the 3 uses - Correct answer 1. count microorganism or cells
2. view stained specimen
3. view live unstained specimen
Bright-field: list the feature(s) - Correct answer staining usually required
,Bright-field: list the types of image(s) - Correct answer bright background with clear
or colored specimen
Bright-field: specs for memorization - Correct answer halogen bulb > light from base
is reflected to sample
Condeser - Correct answer converges light beams into a focused area on the sample
iris diaphragm - Correct answer controls the amount of light that passes through the
sample and into the objective lens
objective lens - Correct answer lens closest to the sample; greatest amount of
magnification (more light = more magnification degree)
ocular lens - Correct answer eyepiece for the eye; (10x)
multicaptive - Correct answer when ocular and objective lens' are used together
, (10x + 40x = 400x magnification)
what is required before staining? - Correct answer heat or chemical methods
Phase Contrast: uses - Correct answer 1. view internal structures of live specimen
2. observe motility (cilia flagella)
specialized
Phase Contrast: features - Correct answer no staining necessary so we can view LIVE
specimens
Phase Contrast: images - Correct answer show light and dark areas of microbe
Phase Contrast: advantages - Correct answer over bright > able to visualize invisible
structures without staining.
