BLOOD BANK PRACTICE EXAMINATION
2026 QUESTIONS WITH ANSWERS
GUARANTEED TO PASS.
⫸ 8) Prior to blood donation, the intended venipuncture site must be
cleaned with a scrub solution of:
a. hypochlorite
b. isopropyl alcohol
c. 10% acetone
d. PVP iodine complex. Ans: d. PVP iodine complex
(The scrub must use iodine, eg, PVP iodine complex. Donors who are
sensitive to iodine can have the area cleaned with a preparation of 2%
chlorhexidine and 70% isopropyl alcohol.)
⫸ 9) All donors blood testing must include:
a. complete Rh phenotype
b. anti-CMV testing
c. DAT
d. serological test for syphilis. Ans: d. serological test for syphilis
,(Testing tor syphilis was the first mandated donor screening test for
infectious disease and is still part of donor screening.)
⫸ 10) During the preparation of Platelet Concentrates from whole
blood, the blood should be:
a. cooled towards 6 C
b. cooled towards 20-24 C
c. warmed to 37 C
d. heated to 57 C. Ans: b. cooled towards 20-24 C
(Platelets are prepared and stored at 20-24 C for optimum function.)
⫸ 12) The western blot is a confirmatory test for:
a. CMV antibody
b. anti-HIV-1
c. HbsAg
d. serum protein abnormality. Ans: b. anti-HIV-1
(Western blot uses purified HIV proteins to confirm reactivity in samples
whose screening test for anti-HIV is positive.)
⫸ 13) The test that is currently used to detect donors who are infected
with the AIDS virus is:
,a. anti-HBc
b. anti-HIV-1,2
c. HbsAg
d. ALT. Ans: b. anti-HIV-1,2
(The causative agent for AIDS is the human immunodeficiency virus
types 1 and 2.)
⫸ 14) A commonly used screening method for anti-HIV detection is:
a. latex agglutination
b. RIA
c. TLC
d. ELISA. Ans: d. ELISA
(The enzyme-labeled immunosorbent assay [ELISA] method is a very
sensitive method employed to screen donors for markers of transfusion-
transmitted viruses.)
⫸ 16) A unit of packed cells is split into 2 aliquots under closed sterile
conditions at 8 am. The expiration time for each aliquot is:
a. 4 pm the same day
b. 8 pm the same say
c. 8 am the next morning
, d. the original date of the unsplit unit. Ans: d. the original date of the
unsplit unit
(Sterile docking devices allow entry into donor units without affecting
the expiration date of the product.)
⫸ 18) When platelets are stored on a rotator set on an open bench top,
the ambient air temps. must be recorded:
a. once a day
b. twice a day
c. every 4 hours
d. every hour. Ans: c. every 4 hours
(If storage devices do not have automated temperature recording,
temperature must he manually monitored every 4 hours.)
⫸ 19) Which of the following is the correct storage temp for the
component listed:
a. Cryo AHF = 4 C
b. FFP = -20 C
c. Frozen RBC = -40 C
d. Platelets = 37 C. Ans: b. FFP = -20 C
2026 QUESTIONS WITH ANSWERS
GUARANTEED TO PASS.
⫸ 8) Prior to blood donation, the intended venipuncture site must be
cleaned with a scrub solution of:
a. hypochlorite
b. isopropyl alcohol
c. 10% acetone
d. PVP iodine complex. Ans: d. PVP iodine complex
(The scrub must use iodine, eg, PVP iodine complex. Donors who are
sensitive to iodine can have the area cleaned with a preparation of 2%
chlorhexidine and 70% isopropyl alcohol.)
⫸ 9) All donors blood testing must include:
a. complete Rh phenotype
b. anti-CMV testing
c. DAT
d. serological test for syphilis. Ans: d. serological test for syphilis
,(Testing tor syphilis was the first mandated donor screening test for
infectious disease and is still part of donor screening.)
⫸ 10) During the preparation of Platelet Concentrates from whole
blood, the blood should be:
a. cooled towards 6 C
b. cooled towards 20-24 C
c. warmed to 37 C
d. heated to 57 C. Ans: b. cooled towards 20-24 C
(Platelets are prepared and stored at 20-24 C for optimum function.)
⫸ 12) The western blot is a confirmatory test for:
a. CMV antibody
b. anti-HIV-1
c. HbsAg
d. serum protein abnormality. Ans: b. anti-HIV-1
(Western blot uses purified HIV proteins to confirm reactivity in samples
whose screening test for anti-HIV is positive.)
⫸ 13) The test that is currently used to detect donors who are infected
with the AIDS virus is:
,a. anti-HBc
b. anti-HIV-1,2
c. HbsAg
d. ALT. Ans: b. anti-HIV-1,2
(The causative agent for AIDS is the human immunodeficiency virus
types 1 and 2.)
⫸ 14) A commonly used screening method for anti-HIV detection is:
a. latex agglutination
b. RIA
c. TLC
d. ELISA. Ans: d. ELISA
(The enzyme-labeled immunosorbent assay [ELISA] method is a very
sensitive method employed to screen donors for markers of transfusion-
transmitted viruses.)
⫸ 16) A unit of packed cells is split into 2 aliquots under closed sterile
conditions at 8 am. The expiration time for each aliquot is:
a. 4 pm the same day
b. 8 pm the same say
c. 8 am the next morning
, d. the original date of the unsplit unit. Ans: d. the original date of the
unsplit unit
(Sterile docking devices allow entry into donor units without affecting
the expiration date of the product.)
⫸ 18) When platelets are stored on a rotator set on an open bench top,
the ambient air temps. must be recorded:
a. once a day
b. twice a day
c. every 4 hours
d. every hour. Ans: c. every 4 hours
(If storage devices do not have automated temperature recording,
temperature must he manually monitored every 4 hours.)
⫸ 19) Which of the following is the correct storage temp for the
component listed:
a. Cryo AHF = 4 C
b. FFP = -20 C
c. Frozen RBC = -40 C
d. Platelets = 37 C. Ans: b. FFP = -20 C
