MLT ASCP CHEMISTRY EXAM WITH
ACCURATE QUESTIONS AND ANSWERS
(VERIFIED ANSWERS) | LATEST
(2026/2027) UPDATED VERSION | 100%
GUARANTEED PASS
{JUST RELEASED}
1. Which one of the following statements about acetaminophen metabolism is
false?
Choose the single best answer
A. It is metabolized in the kidney
B. It is largely metabolized in the liver to glucuronide and sulfonate
conjugates
C. A small amount is metabolized via cytochrome oxidase to a reactive
benzoquinoneimine intermediate
D. This intermediate becomes toxic to the liver once tissue glutathione
becomes depleted - ANSWER ✓ A;
, The benzoquinoneimine intermediate produced in the liver as a result of
hepatic cytochrome oxidase metabolism of acetaminophen is responsible for
hepatic necrosis via acylation of hepatic tissue. The treatment of
acetaminophen toxicity includes the use of N-acetylcysteine (Mucomyst),
which acts as a glutathione substitute. The risk of hepatotoxicity is predicted
with the Rumack-Matthew nomogram, which uses the hours after ingestion
and plasma acetaminophen level to predict the probability of hepatotoxicity.
Severe hepatotoxicity can be fatal.
2. According to the 2010 ADA guidelines, HbA1C percent can be used as an assay
to diagnose diabetes.
True/False - ANSWER ✓ T;
The 2010 ADA Clinical Practice Recommendations added measurement of
HbA1C as a diagnostic assay for diagnosis of diabetes.
3. Which of the following conditions would make a sample unsuitable for lactic
acid testing?
Choose the single best answer
A. Movement of hand or arm during specimen collection
B. Received in the lab on ice
C. Centrifuged in a refrigerated centrifuge
, D. Specimen centrifuged and plasma separated from cells less than 15
minutes after collection - ANSWER ✓ A;
Movement of the muscles in the hand or arm during specimen collection will
falsely increase lactic acid concentration. A blood sample for lactic acid testing
should be placed on ice immediately after collection, centrifuged within 15
minutes of collection, and plasma immediately separated from cells following
centrifugation. If possible, a refrigerated centrifuge should be used.
4. You are working in a clinical chemistry laboratory and are analyzing a plasma
glucose sample. The sample is flagged by the analyzer for being "outside of
linear range." You manually dilute the sample 1:2 and rerun it. Again, you
receive an "outside linear range" alert. You decide to perform a different
manual dilution. This time you manually dilute the original sample again; this
time using a 1:3 dilution. The instrument gives you a glucose value of 150
mg/dL from this diluted sample. What is the actual patient glucose result that
you should report to the physician?
Choose the single best answer
A. 100 mg/dL
B. 400 mg/dL
C. 600 mg/dL
D. 450 mg/dL
E. 1200 mg/dL - ANSWER ✓ D;
, The correct response is option D: 450 mg/dl. A 1:3 dilution entails mixing one
part patient sample with two parts diluent, for a total volume of 3 parts. In
laboratory dilutions, the total volume is considered a dilution factor. The
dilution factor in this question is 3. So a diluted sample with a value of 150
would need to be multiplied by 3 to obtain the correct final answer. 150 x 3 =
450 mg/dl.
5. That portion of an enzyme which is separated from its cofactor is called a(n):
Choose the single best answer
A. Partial enzyme
B. Isoenzyme
C. Coenzyme
D. Apoenzyme - ANSWER ✓ D;
A partial enzyme is not an actual term used in the laboratory. An isoenzyme is
a related enzyme with a different chemical structure. Finally, a coenzyme is a
non-protein molecule (often a vitamin) that helps an enzyme become active.
6. A low CSF glucose level is associated with all the following except:
Choose the single best answer
ACCURATE QUESTIONS AND ANSWERS
(VERIFIED ANSWERS) | LATEST
(2026/2027) UPDATED VERSION | 100%
GUARANTEED PASS
{JUST RELEASED}
1. Which one of the following statements about acetaminophen metabolism is
false?
Choose the single best answer
A. It is metabolized in the kidney
B. It is largely metabolized in the liver to glucuronide and sulfonate
conjugates
C. A small amount is metabolized via cytochrome oxidase to a reactive
benzoquinoneimine intermediate
D. This intermediate becomes toxic to the liver once tissue glutathione
becomes depleted - ANSWER ✓ A;
, The benzoquinoneimine intermediate produced in the liver as a result of
hepatic cytochrome oxidase metabolism of acetaminophen is responsible for
hepatic necrosis via acylation of hepatic tissue. The treatment of
acetaminophen toxicity includes the use of N-acetylcysteine (Mucomyst),
which acts as a glutathione substitute. The risk of hepatotoxicity is predicted
with the Rumack-Matthew nomogram, which uses the hours after ingestion
and plasma acetaminophen level to predict the probability of hepatotoxicity.
Severe hepatotoxicity can be fatal.
2. According to the 2010 ADA guidelines, HbA1C percent can be used as an assay
to diagnose diabetes.
True/False - ANSWER ✓ T;
The 2010 ADA Clinical Practice Recommendations added measurement of
HbA1C as a diagnostic assay for diagnosis of diabetes.
3. Which of the following conditions would make a sample unsuitable for lactic
acid testing?
Choose the single best answer
A. Movement of hand or arm during specimen collection
B. Received in the lab on ice
C. Centrifuged in a refrigerated centrifuge
, D. Specimen centrifuged and plasma separated from cells less than 15
minutes after collection - ANSWER ✓ A;
Movement of the muscles in the hand or arm during specimen collection will
falsely increase lactic acid concentration. A blood sample for lactic acid testing
should be placed on ice immediately after collection, centrifuged within 15
minutes of collection, and plasma immediately separated from cells following
centrifugation. If possible, a refrigerated centrifuge should be used.
4. You are working in a clinical chemistry laboratory and are analyzing a plasma
glucose sample. The sample is flagged by the analyzer for being "outside of
linear range." You manually dilute the sample 1:2 and rerun it. Again, you
receive an "outside linear range" alert. You decide to perform a different
manual dilution. This time you manually dilute the original sample again; this
time using a 1:3 dilution. The instrument gives you a glucose value of 150
mg/dL from this diluted sample. What is the actual patient glucose result that
you should report to the physician?
Choose the single best answer
A. 100 mg/dL
B. 400 mg/dL
C. 600 mg/dL
D. 450 mg/dL
E. 1200 mg/dL - ANSWER ✓ D;
, The correct response is option D: 450 mg/dl. A 1:3 dilution entails mixing one
part patient sample with two parts diluent, for a total volume of 3 parts. In
laboratory dilutions, the total volume is considered a dilution factor. The
dilution factor in this question is 3. So a diluted sample with a value of 150
would need to be multiplied by 3 to obtain the correct final answer. 150 x 3 =
450 mg/dl.
5. That portion of an enzyme which is separated from its cofactor is called a(n):
Choose the single best answer
A. Partial enzyme
B. Isoenzyme
C. Coenzyme
D. Apoenzyme - ANSWER ✓ D;
A partial enzyme is not an actual term used in the laboratory. An isoenzyme is
a related enzyme with a different chemical structure. Finally, a coenzyme is a
non-protein molecule (often a vitamin) that helps an enzyme become active.
6. A low CSF glucose level is associated with all the following except:
Choose the single best answer
