BIOL 1503- LAB FINAL QUESTIONS WITH VERIFIED
ANSWERS
What is the organism we will conduct research on in this class? - answers - eastern
oysters
True or false: natural selection is random and goal oriented? - answers - false: natural
selection is random but it is not goal oriented.
________ is the change in the genetic makeup of a population over time - answers -
evolution
Name 2 ways in which scientists communicate their research - answers - journals,
posters
Name 2 topics we will cover on week 2 of class. - answers - pipetting, reading scientific
literature
Define the terms "allele" and "gene" - answers - gene- location on a chromosome that
codes for a specific trait
Allele- alternitive forms of a gene
How many stops are there on a pipette? What is each one used for? - answers - 2
stops.
1st is dispensing and capturing liquid
2nd is to make sure all of the liquid is expelled
Dna extraction: what are "proteinase k" and "rnase a" used for? - answers - proteinase k
is used to break down and wash out any leftover protien
Rnase a is used to break down and wash out any leftover rna
What is the purpose of pcr? - answers - dna amplification (making more dna)
Name 2 constituents (ingredients) of a pcr reaction and what they are used for? -
answers - master mix- nucleotides and polymerase to bind the denatured dna
Reverse primer- attaches to specific area of dna to start a reaction
What charge does dna have? Why does it have this charge? - answers - negative,
because the phosphate is negatively charged
What is the purpose of gel electrophoresis? - answers - to separate out bands, confirm
that the pcr was effective, look for the correct length of bands
ANSWERS
What is the organism we will conduct research on in this class? - answers - eastern
oysters
True or false: natural selection is random and goal oriented? - answers - false: natural
selection is random but it is not goal oriented.
________ is the change in the genetic makeup of a population over time - answers -
evolution
Name 2 ways in which scientists communicate their research - answers - journals,
posters
Name 2 topics we will cover on week 2 of class. - answers - pipetting, reading scientific
literature
Define the terms "allele" and "gene" - answers - gene- location on a chromosome that
codes for a specific trait
Allele- alternitive forms of a gene
How many stops are there on a pipette? What is each one used for? - answers - 2
stops.
1st is dispensing and capturing liquid
2nd is to make sure all of the liquid is expelled
Dna extraction: what are "proteinase k" and "rnase a" used for? - answers - proteinase k
is used to break down and wash out any leftover protien
Rnase a is used to break down and wash out any leftover rna
What is the purpose of pcr? - answers - dna amplification (making more dna)
Name 2 constituents (ingredients) of a pcr reaction and what they are used for? -
answers - master mix- nucleotides and polymerase to bind the denatured dna
Reverse primer- attaches to specific area of dna to start a reaction
What charge does dna have? Why does it have this charge? - answers - negative,
because the phosphate is negatively charged
What is the purpose of gel electrophoresis? - answers - to separate out bands, confirm
that the pcr was effective, look for the correct length of bands
