BLOOD BANK PRACTICE EXAM 2026 CORE
QUESTIONS AND ACCURATE ANSWERS
ANTIBODY SCREENING TRAINING PACK
◉ What is the cause of febrile transfusion reactions
Answer: Leukoctye antibodies present in the patient's plasma that
react with leukocytes in the blood
◉ Which of the following antibodies are implicated in delayed
hemolytic transfusion reactions
Answer: Anti-A1, anti-P1, anti-Jk(a)
◉ Urticarial transfusion reactions have no definitive cause, but it is
thought IgE is involved. What are the symptoms
Answer: pruritus
hives
erythema
◉ TRALI and TACO have similar symptoms, but they differ by which
of the following
Answer: TACO patients suffer from hypervolemia and TRALI
patients do not.
,◉ Which of the following is an iatrogenic transfusion reaction(If you
want to get technical, I guess you could say they are all iatrogenic,
but your text does focus on one.)
Answer: Transfusion Associated Circulatory Overload
◉ What is the first procedure to be done in the laboratory
investigation of a transfusion reaction
Answer: Clerical check on all the paper work.
◉ If the Direct antiglobulin test is positive due to IgG, how will this
look microscopically and what test must be done to identify the IgG
antibody on the cells
Answer: It will look mixed field microscopically and the IgG antibody
must be eluted from the cells. Test this eluate against a panel to
identify the antibody.
◉ If a patient has febrile transfusion reactions, how are future
transfusions handled
Answer: Leukoreduce the cellular products to be infused.
◉ What must be done to prevent graft-versus-host disease
Answer: Irradiate the cellular products to be infused.
, ◉ If a patient has anaphylactic transfusion reactions, how are future
transfusions handled
Answer: Wash the cellular products to be infused.
◉ For both the DAT and the IAT the cell button should be washed at
least three times prior to adding AHG. Why
Answer: To remove globulins from the serum/plasma surrounding
the cells
◉ The amount of antigen present on a cell influences the first stage
of agglutination. Which of the following cells has the most antigen
Answer: A cell that is homozygous for the antigen
◉ What is antihuman globulin
Answer: An IgG antibody against human globulin
◉ What is the ABO type of check cells
Answer: O
◉ What is the first stage of agglutination called
Answer: Sensitization
◉ What is the purpose of the Direct Antiglobulin Test (DAT)
QUESTIONS AND ACCURATE ANSWERS
ANTIBODY SCREENING TRAINING PACK
◉ What is the cause of febrile transfusion reactions
Answer: Leukoctye antibodies present in the patient's plasma that
react with leukocytes in the blood
◉ Which of the following antibodies are implicated in delayed
hemolytic transfusion reactions
Answer: Anti-A1, anti-P1, anti-Jk(a)
◉ Urticarial transfusion reactions have no definitive cause, but it is
thought IgE is involved. What are the symptoms
Answer: pruritus
hives
erythema
◉ TRALI and TACO have similar symptoms, but they differ by which
of the following
Answer: TACO patients suffer from hypervolemia and TRALI
patients do not.
,◉ Which of the following is an iatrogenic transfusion reaction(If you
want to get technical, I guess you could say they are all iatrogenic,
but your text does focus on one.)
Answer: Transfusion Associated Circulatory Overload
◉ What is the first procedure to be done in the laboratory
investigation of a transfusion reaction
Answer: Clerical check on all the paper work.
◉ If the Direct antiglobulin test is positive due to IgG, how will this
look microscopically and what test must be done to identify the IgG
antibody on the cells
Answer: It will look mixed field microscopically and the IgG antibody
must be eluted from the cells. Test this eluate against a panel to
identify the antibody.
◉ If a patient has febrile transfusion reactions, how are future
transfusions handled
Answer: Leukoreduce the cellular products to be infused.
◉ What must be done to prevent graft-versus-host disease
Answer: Irradiate the cellular products to be infused.
, ◉ If a patient has anaphylactic transfusion reactions, how are future
transfusions handled
Answer: Wash the cellular products to be infused.
◉ For both the DAT and the IAT the cell button should be washed at
least three times prior to adding AHG. Why
Answer: To remove globulins from the serum/plasma surrounding
the cells
◉ The amount of antigen present on a cell influences the first stage
of agglutination. Which of the following cells has the most antigen
Answer: A cell that is homozygous for the antigen
◉ What is antihuman globulin
Answer: An IgG antibody against human globulin
◉ What is the ABO type of check cells
Answer: O
◉ What is the first stage of agglutination called
Answer: Sensitization
◉ What is the purpose of the Direct Antiglobulin Test (DAT)
