Life Science: W10 PEQs Test Questions
and All Correct Answers.
Shown below is the start of a coding region within the first exon of a gene. Which sequence
could you use for your sgRNA?
5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'
3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'
(a) 3' CGACACAGCATGTCAAGAGA 5'
(b) 5' CGACACAGCATGTCAAGAGA 3'
(c) 3' GCTGTGTCGTACAGTTCTCT 5'
(d) 5' GCTGTGTCGTACAGTTCTCT 3' - Answer (c) 3' GCTGTGTCGTACAGTTCTCT 5'
Shown below is the start of a coding region within the first exon of a gene. How many Cas9 PAM
sequences are present?
5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'
3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'
(a) 0
(b) 3
(c) 2
(d) 4
(e) 1 - Answer (c) 2
Shown below is part of a coding region within the first exon of a gene. Where will Cas9 cause a
double stranded DNA break?
(a) B
(b) A
(c) D
(d) C - Answer (b) A
and All Correct Answers.
Shown below is the start of a coding region within the first exon of a gene. Which sequence
could you use for your sgRNA?
5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'
3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'
(a) 3' CGACACAGCATGTCAAGAGA 5'
(b) 5' CGACACAGCATGTCAAGAGA 3'
(c) 3' GCTGTGTCGTACAGTTCTCT 5'
(d) 5' GCTGTGTCGTACAGTTCTCT 3' - Answer (c) 3' GCTGTGTCGTACAGTTCTCT 5'
Shown below is the start of a coding region within the first exon of a gene. How many Cas9 PAM
sequences are present?
5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'
3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'
(a) 0
(b) 3
(c) 2
(d) 4
(e) 1 - Answer (c) 2
Shown below is part of a coding region within the first exon of a gene. Where will Cas9 cause a
double stranded DNA break?
(a) B
(b) A
(c) D
(d) C - Answer (b) A
