Test 8 Phlebotomy Q&A 2026 Complete
Study Guide.
This document details the proper procedures for
collecting blood cultures, including skin preparation, bottle
selection, and order of draw. It also covers common
collection problems and laboratory processing.
# Term Definition
1 What is the primary Blood cultures are lab tests used
purpose of blood to check for bacteria or other
cultures? microorganisms in a blood
sample, often ordered to
diagnose Fever of Unknown
Origin (FUO).
2 Who is responsible for The microbiology department is
processing blood responsible for processing blood
cultures? cultures.
3 What is the main If microorganisms grow, the
goal when test can help doctors determine
microorganisms grow which antibiotics will be most
in a blood culture? effective for treating the
infection.
4 What is the general Adult blood culture bottles
requirement for adult generally require 8-10ml of
blood culture bottles? blood.
5 What is the fill Pediatric blood culture bottles
volume for pediatric require a fill volume of 1-3ml of
blood culture bottles? blood and typically only one bottle
is used.
6 What are the two main Blood cultures are typically
methods for collecting collected using butterfly needles
1
, blood cultures? or syringes.
7 What is the specific If using the syringe method, draw
order for drawing blood the Anaerobic bottle first,
cultures using a followed by the Aerobic bottle.
syringe?
8 What is the specific If using the butterfly method, draw
order for drawing blood the Aerobic bottle first, followed
cultures using a butterfly by the Anaerobic bottle.
needle?
9 What is the first Clean the tops of both blood
step in skin culture bottles or SPS tubes with
preparation for 70% isopropyl alcohol and allow
blood culture them to air dry.
collection?
10 Describe the skin Scrub the skin with 70% isopropyl
scrubbing procedure alcohol using a back and forth
using 70% isopropyl motion for 1 minute and allow it
alcohol for blood to air dry. The site should be
cultures. cleansed to a diameter of about
6.35cm (2.5in).
2
Study Guide.
This document details the proper procedures for
collecting blood cultures, including skin preparation, bottle
selection, and order of draw. It also covers common
collection problems and laboratory processing.
# Term Definition
1 What is the primary Blood cultures are lab tests used
purpose of blood to check for bacteria or other
cultures? microorganisms in a blood
sample, often ordered to
diagnose Fever of Unknown
Origin (FUO).
2 Who is responsible for The microbiology department is
processing blood responsible for processing blood
cultures? cultures.
3 What is the main If microorganisms grow, the
goal when test can help doctors determine
microorganisms grow which antibiotics will be most
in a blood culture? effective for treating the
infection.
4 What is the general Adult blood culture bottles
requirement for adult generally require 8-10ml of
blood culture bottles? blood.
5 What is the fill Pediatric blood culture bottles
volume for pediatric require a fill volume of 1-3ml of
blood culture bottles? blood and typically only one bottle
is used.
6 What are the two main Blood cultures are typically
methods for collecting collected using butterfly needles
1
, blood cultures? or syringes.
7 What is the specific If using the syringe method, draw
order for drawing blood the Anaerobic bottle first,
cultures using a followed by the Aerobic bottle.
syringe?
8 What is the specific If using the butterfly method, draw
order for drawing blood the Aerobic bottle first, followed
cultures using a butterfly by the Anaerobic bottle.
needle?
9 What is the first Clean the tops of both blood
step in skin culture bottles or SPS tubes with
preparation for 70% isopropyl alcohol and allow
blood culture them to air dry.
collection?
10 Describe the skin Scrub the skin with 70% isopropyl
scrubbing procedure alcohol using a back and forth
using 70% isopropyl motion for 1 minute and allow it
alcohol for blood to air dry. The site should be
cultures. cleansed to a diameter of about
6.35cm (2.5in).
2
