H AND R BLOCK TAX PROFESSIONAL
FINAL SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
⩥thioester bond (1). Answer: compounds with functional group C-S-
CoA-C (eg, in acetyl-CoA)
⩥zwitterion (3). Answer: dipolar ion with both positive and negative
groups but overall neutral charge; can act as either an acid or base
⩥Isoelectric point (pI) (chapter 3). Answer: the characteristic pH at
which the net electric charge is zero
⩥SDS - Sodium Dodecyl Sulfate (chap 3). Answer: detergent used to
unfold proteins and give them uniform negative charge
⩥SDS Page (3). Answer: chromatography used to separate proteins
based on mass. light proteins travel fast than heavier ones
⩥PCR - Polymerase chain reaction (3). Answer: copies DNA multiple
times to increase sample size
, ⩥Isoelectric focusing (chapt 3). Answer: procedure used to determine
the isoelectric pt (pI) of a protein. Protein migrates through gel until pH
= pI (net charge = 0)
⩥Two-Dimensional Electrophoresis (3). Answer: combines isoelectric
focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
⩥specific activity (3). Answer: number of enzyme units per mg of total
protein (a measure of enzyme purity)
⩥activity (3). Answer: total units of a certain enzyme in a solution
⩥Peptide bonds (chapter 4). Answer: C-N bond with double bond
character due to resonance (C-N bond cannot rotate, and is planar)
⩥Edman degredation (3). Answer: used in the sequencing of
polypeptides; labels and removes ONLY the amino-residue from a
polypeptide. carried out in a machine called a sequenator
⩥φ in peptide bonding (chapter 4). Answer: angle around the α-carbon -
amide nitrogen bond
⩥ψ in peptide bonding (chapter 4). Answer: angle around the α-carbon -
carbonyl carbon bond
FINAL SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
⩥thioester bond (1). Answer: compounds with functional group C-S-
CoA-C (eg, in acetyl-CoA)
⩥zwitterion (3). Answer: dipolar ion with both positive and negative
groups but overall neutral charge; can act as either an acid or base
⩥Isoelectric point (pI) (chapter 3). Answer: the characteristic pH at
which the net electric charge is zero
⩥SDS - Sodium Dodecyl Sulfate (chap 3). Answer: detergent used to
unfold proteins and give them uniform negative charge
⩥SDS Page (3). Answer: chromatography used to separate proteins
based on mass. light proteins travel fast than heavier ones
⩥PCR - Polymerase chain reaction (3). Answer: copies DNA multiple
times to increase sample size
, ⩥Isoelectric focusing (chapt 3). Answer: procedure used to determine
the isoelectric pt (pI) of a protein. Protein migrates through gel until pH
= pI (net charge = 0)
⩥Two-Dimensional Electrophoresis (3). Answer: combines isoelectric
focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
⩥specific activity (3). Answer: number of enzyme units per mg of total
protein (a measure of enzyme purity)
⩥activity (3). Answer: total units of a certain enzyme in a solution
⩥Peptide bonds (chapter 4). Answer: C-N bond with double bond
character due to resonance (C-N bond cannot rotate, and is planar)
⩥Edman degredation (3). Answer: used in the sequencing of
polypeptides; labels and removes ONLY the amino-residue from a
polypeptide. carried out in a machine called a sequenator
⩥φ in peptide bonding (chapter 4). Answer: angle around the α-carbon -
amide nitrogen bond
⩥ψ in peptide bonding (chapter 4). Answer: angle around the α-carbon -
carbonyl carbon bond
