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Exam Solution
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Micro Exam 3 Portage Learning 2026 A+ GRADE ASSUR
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ED COMPLETE SOLUTIONS AND VERIFIED ANSWERS (E
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B6FE)
QUESTION 1 zm
Resolution and contrast are two critical factors that influence your ability to see an o
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bject. Explain Each.
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ANSWER
Resolution refer to the distance between two objects and can still be seen as separate. Contrast is t
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he difference in light absorbance between two objects. Poor contrast gives a high background and m
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akes visualization of multiple objects difficult.
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QUESTION 2 zm
What is the total magnification (relative to your eye) of a sample imaged with a 60x o
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bjective and 10x eyepiece? Show your math
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ANSWER
60x10=600x magnification zm
QUESTION 3 zm
For each of the following questions select from the list below the single best answer:
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ANSWER
Fluoresence= This type of microscope is best suited for visualizing GFP, RFP, and YFP proteins Phas
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e-
Contrast= This type of microscope can provide detailed images of live cells without straining. Dark F
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ield= This type of microscope is used to greatly increase the contrast between samples and backgro
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und by reflecting light off of the specimen. Conofocal= This type of microscope is capable of capturi
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ng images in multiple focal planes, rendering, a specimen in 3D
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Exam Solution
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Micro Exam 3 Portage Learning 2026 A+ GRADE ASSUR
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ED COMPLETE SOLUTIONS AND VERIFIED ANSWERS (E
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B6FE)
QUESTION 1 zm
Resolution and contrast are two critical factors that influence your ability to see an o
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bject. Explain Each.
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ANSWER
Resolution refer to the distance between two objects and can still be seen as separate. Contrast is t
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he difference in light absorbance between two objects. Poor contrast gives a high background and m
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akes visualization of multiple objects difficult.
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QUESTION 2 zm
What is the total magnification (relative to your eye) of a sample imaged with a 60x o
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bjective and 10x eyepiece? Show your math
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ANSWER
60x10=600x magnification zm
QUESTION 3 zm
For each of the following questions select from the list below the single best answer:
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ANSWER
Fluoresence= This type of microscope is best suited for visualizing GFP, RFP, and YFP proteins Phas
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e-
Contrast= This type of microscope can provide detailed images of live cells without straining. Dark F
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ield= This type of microscope is used to greatly increase the contrast between samples and backgro
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und by reflecting light off of the specimen. Conofocal= This type of microscope is capable of capturi
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ng images in multiple focal planes, rendering, a specimen in 3D
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