ANSC 333 ACTUAL EXAM SCRIPT 2026
COMPREHENSIVE QUESTIONS AND
ANSWERS
◉ go in earlier when oocyte is starting to develop antrum, but NO
inhibin- oocyte is not ready to be fertilized
- undergo in vitro maturation for 24 hours then introduce sperm to
Petri dish
Answer: What is the new way to do IVF?
◉ less efficient- 1 oocyte= .2 embryos
Answer: What is the downside to the new IVF?
◉ cheaper and greater opportunity
Answer: Why is the new IVF starting to take over?
◉ controlled time/temp descent- slowly decrease the temp of the
embryos over time
Answer: Cryopreservation
◉ displace water cells to prevent damage
,Answer: Why must cryoprotectants be added during
cryopreservation?
◉ pro: cryoprotectants are toxic and you don't have to do much
con: ice crystals can form and can be damaging
Answer: What are the pros and cons to cryopreservation?
◉ solidification without ice crystal formation
Answer: vitrification
◉ pro: no ice crystals
con: must add a lot of toxin cryopresevitative
Answer: What are the pros and cons of vitrification
◉ pig embryos cannot be cryopreserved using old methods because
they have a high lipid content in the embryo
Answer: Why was vitrification created?
◉ taking an embryo and slicing it in half with a micro-manipulator
blade and transferring into recipient
Answer: embryo splitting
, ◉ compacted morula
Answer: What kind of embryo do you want for embryo splitting?
◉ must equally split ICM and trophoblasts
Answer: Why are compacted morulas used for embryo splitting?
◉ remove part of blastomere and genotype to determine if genetic
defects exist
Answer: What is embryo genotyping/sexing?
◉ incorporate genes of species that aren't normally there, OR
express genes that wouldn't normally be expressed
Answer: What is transgenic manipulation?
◉ viral vector, CRISPR
Answer: What are examples of transgenic manipulation?
◉ in pigs: add phytase gene to pig DNA so the pigs will produce
more phytase enzyme, decreased the amount of P excreted, positive
result in the environment
Answer: Where is transgenic manipulation be applied?
COMPREHENSIVE QUESTIONS AND
ANSWERS
◉ go in earlier when oocyte is starting to develop antrum, but NO
inhibin- oocyte is not ready to be fertilized
- undergo in vitro maturation for 24 hours then introduce sperm to
Petri dish
Answer: What is the new way to do IVF?
◉ less efficient- 1 oocyte= .2 embryos
Answer: What is the downside to the new IVF?
◉ cheaper and greater opportunity
Answer: Why is the new IVF starting to take over?
◉ controlled time/temp descent- slowly decrease the temp of the
embryos over time
Answer: Cryopreservation
◉ displace water cells to prevent damage
,Answer: Why must cryoprotectants be added during
cryopreservation?
◉ pro: cryoprotectants are toxic and you don't have to do much
con: ice crystals can form and can be damaging
Answer: What are the pros and cons to cryopreservation?
◉ solidification without ice crystal formation
Answer: vitrification
◉ pro: no ice crystals
con: must add a lot of toxin cryopresevitative
Answer: What are the pros and cons of vitrification
◉ pig embryos cannot be cryopreserved using old methods because
they have a high lipid content in the embryo
Answer: Why was vitrification created?
◉ taking an embryo and slicing it in half with a micro-manipulator
blade and transferring into recipient
Answer: embryo splitting
, ◉ compacted morula
Answer: What kind of embryo do you want for embryo splitting?
◉ must equally split ICM and trophoblasts
Answer: Why are compacted morulas used for embryo splitting?
◉ remove part of blastomere and genotype to determine if genetic
defects exist
Answer: What is embryo genotyping/sexing?
◉ incorporate genes of species that aren't normally there, OR
express genes that wouldn't normally be expressed
Answer: What is transgenic manipulation?
◉ viral vector, CRISPR
Answer: What are examples of transgenic manipulation?
◉ in pigs: add phytase gene to pig DNA so the pigs will produce
more phytase enzyme, decreased the amount of P excreted, positive
result in the environment
Answer: Where is transgenic manipulation be applied?
