BIO250L CERTIFICATION EVALUATION
TEST PAPER FULL SOLUTION QUESTIONS
AND ANSWERS PACK
●● define the medium?
Answer: liquid or gel designed to support bacterial/cell growth
●● what are the two types if medium and what do they provide?
Answer: broth(liquid) and agar(solid);
- they provide stable growth conditions
-limit nutrients to bacteria
●● what three ways do you handle media?
Answer: make sure that the top is on the bottom; plate is upside down so
moisture doesnt drip onto surface and dirupt micrbobial growth
make sure to seal the plate with parafilm
store in correct temp for the bacteria
●● define agar
Answer: chemical derived from seaweed that solidifies into a jelly-like
substance and becomes a solid
, ●● what are two types of agar?
Answer: plates and slants
●● example of agar?
Answer: TSA--- trypticase soy agar
-a general medium and all bacteria will grow on them
●● what is the air exposure procedure?
Answer: open the plate to expose it to the air for several minutes then
seal with parafilm and place in an incubate at 37C for 24 hours then 4C
until the next week
●● what is the objective of a surface swab?
Answer: to determine if cultural microbes are on a surface of your
choice but your body
●● what is sterilization and some examples?
Answer: when you kill all the microbial contaminants;
autoclave and radiation
-only 1 fails in 1 billion times
●● what is autoclave?
TEST PAPER FULL SOLUTION QUESTIONS
AND ANSWERS PACK
●● define the medium?
Answer: liquid or gel designed to support bacterial/cell growth
●● what are the two types if medium and what do they provide?
Answer: broth(liquid) and agar(solid);
- they provide stable growth conditions
-limit nutrients to bacteria
●● what three ways do you handle media?
Answer: make sure that the top is on the bottom; plate is upside down so
moisture doesnt drip onto surface and dirupt micrbobial growth
make sure to seal the plate with parafilm
store in correct temp for the bacteria
●● define agar
Answer: chemical derived from seaweed that solidifies into a jelly-like
substance and becomes a solid
, ●● what are two types of agar?
Answer: plates and slants
●● example of agar?
Answer: TSA--- trypticase soy agar
-a general medium and all bacteria will grow on them
●● what is the air exposure procedure?
Answer: open the plate to expose it to the air for several minutes then
seal with parafilm and place in an incubate at 37C for 24 hours then 4C
until the next week
●● what is the objective of a surface swab?
Answer: to determine if cultural microbes are on a surface of your
choice but your body
●● what is sterilization and some examples?
Answer: when you kill all the microbial contaminants;
autoclave and radiation
-only 1 fails in 1 billion times
●● what is autoclave?
