AAB MOLECULAR DIAGNOSTICS FINAL
PAPER 2026 COMPREHENSIVE SOLUTION
COMPILATION
◉ What is a method for removing nucleotides and primers from PCR
products prior to sequencing?
Answer: Shrimp alkaline phosphatase (SAP) and exonuclease I
(ExoI)
◉ How does nested PCR increase both specificity and sensitivity?
Answer: By using two pairs of primers to amplify a single target in
two separate PCR runs. The second pair of primers bind slightly
inside the binding sites of the first pair. This will increase the
intended PCR product.
◉ How does PCR differ from qPCR (real time)?
Answer: PCR detects the final product by endpoint analysis.
◉ How does qPCR differ from PCR?
Answer: qPCR detects the quantity of product as it is produced
during the run.
, ◉ What is the purpose of the quencher in qPCR?
Answer: To reduce the florescence signal.
◉ What are three common molecular tests that involve the
amplification of probe sequences?
Answer: Ligase Chain Reaction (LCR), Strand Displacement
Amplification (SDA), and Q-beta Replicase.
◉ What is isothermal amplification?
Answer: A process that uses one temperature for amplification after
the initial denaturation.
◉ The major amplification products in Strand Displacement
Amplification (SDA) is?
Answer: Probes/Primers
◉ Strand Displacement Amplification (SDA) was first widely used to
detect which organism?
Answer: Mycobacterium tuberculosis
◉ Where does Q-beta Replicase derives its name?
Answer: From the major enzyme that is used to amplify the probe
sequence (Q-beta replicase)
PAPER 2026 COMPREHENSIVE SOLUTION
COMPILATION
◉ What is a method for removing nucleotides and primers from PCR
products prior to sequencing?
Answer: Shrimp alkaline phosphatase (SAP) and exonuclease I
(ExoI)
◉ How does nested PCR increase both specificity and sensitivity?
Answer: By using two pairs of primers to amplify a single target in
two separate PCR runs. The second pair of primers bind slightly
inside the binding sites of the first pair. This will increase the
intended PCR product.
◉ How does PCR differ from qPCR (real time)?
Answer: PCR detects the final product by endpoint analysis.
◉ How does qPCR differ from PCR?
Answer: qPCR detects the quantity of product as it is produced
during the run.
, ◉ What is the purpose of the quencher in qPCR?
Answer: To reduce the florescence signal.
◉ What are three common molecular tests that involve the
amplification of probe sequences?
Answer: Ligase Chain Reaction (LCR), Strand Displacement
Amplification (SDA), and Q-beta Replicase.
◉ What is isothermal amplification?
Answer: A process that uses one temperature for amplification after
the initial denaturation.
◉ The major amplification products in Strand Displacement
Amplification (SDA) is?
Answer: Probes/Primers
◉ Strand Displacement Amplification (SDA) was first widely used to
detect which organism?
Answer: Mycobacterium tuberculosis
◉ Where does Q-beta Replicase derives its name?
Answer: From the major enzyme that is used to amplify the probe
sequence (Q-beta replicase)
