RID quiz
RID - answerRadial immunodiffusion
RID kit is intended for measurement of human ______ in ______. - answerigG, igA, or
igM in serum or other biological fluids.
In this lab you will be ________. - answerquantitating igG
The method involves antigen (____________) diffusing radially from a cylindrical well
through an agars gel containing a ___________. - answer(igG protein in the sample) ;
monospecific antibody to igG protein.
______ complexes are formed which, under the right conditions, will form a ________. -
answerantigen-antibody; precipitin ring
The ring size will increase until EQUILIBRIUM is reached between the formation of
these complexes. This is called______ or _____. - answer"point of completion" or "zone
of equivalence"
At "point of completion" or "zone of equivalence" a relationship exists between the
__________ and _______ and the______. - answersquare of the ring AND diameter
AND THE antigen concentration.
By measuring the ring diameters produced by a number of samples of KNOWN
CONCENTRATION, a ________ can be constructed. - answercalibration curve
Measuring the ring diameter produced by a sample and reading off a CALIBRATION
CURVE may then determine the ____________ in an UNKNOWN sample. -
answerconcentration of the antigen igG.
Precipitation involves combination of _________ with ______ to produce ______ that
are visible. - answersoluble antigen WITH soluble antibody TO PRODUCE insoluble
complexes.
Agglutination, the technique used to identify ______ involves ________ that aggregate
to form larger complexes. - answerred cell antigens ; particulate antigens
_______ methods are commonly used in the laboratory - answerprecipitation
Procedures that are automated and results can be determined in less time. -
answerNephelometry and Turbimetry
, _________ has largely replaced RID in determining_______. - answerImmunofixation
electrophoresis ; serum immunoglobulin levels
RID. Replace lid and incubate at _________ a minimum of _______. - answerroom
temperature; 48-72 hours.
Measure the ring diameter tot he nearest ________. Record each value. -
answer0.1mm
_______ the value of the ring diameters for the ______. - answerSquare ; calibrators
Use the graphing paper to plot the ________. - answercalibrator values
The ______ should be along the horizontal X axis and the ______ should be along the
vertical Y axis. - answerimmunoglobulin concentrations ; ring diameters
The line of best fit is drawn through these _______. - answer3 points
Determine the concentration of the _______ in each test sample by first ________. -
answerimmunoglobulin; squaring the ring diameter
Find the value of the ______ on the Y axis then draw a line to the _______ then down
to the X axis to find the sample ___________. - answerdiameter; calibration curve;
concentration of the immunoglobulin.
Normal range of igG: _________ mg/dl _______ IU/ml - answer700-1700 ; 80-195
In normal adults, igG constituents approximately _____ of the total _______. -
answer75% ; serum immunoglobulins
The majority of igG is synthesized in the _____. - answerplasma cells
igG can pass the ______ and is critical for the ______ protection until its immune
system develops. - answerplacenta ; neonatal
Serum igG levels are associated with ________ and ______. - answerchronic liver
disease and myeloma (cancer of plasma cells)
Reduced levels may be associated with ________ and other severe _______. -
answermalignancy; severe protein-losing conditions
In this technique, where is the antigen and where is the antibody? - answerantigen= igG
placed in wells
antibody= anti-igG in gel
RID - answerRadial immunodiffusion
RID kit is intended for measurement of human ______ in ______. - answerigG, igA, or
igM in serum or other biological fluids.
In this lab you will be ________. - answerquantitating igG
The method involves antigen (____________) diffusing radially from a cylindrical well
through an agars gel containing a ___________. - answer(igG protein in the sample) ;
monospecific antibody to igG protein.
______ complexes are formed which, under the right conditions, will form a ________. -
answerantigen-antibody; precipitin ring
The ring size will increase until EQUILIBRIUM is reached between the formation of
these complexes. This is called______ or _____. - answer"point of completion" or "zone
of equivalence"
At "point of completion" or "zone of equivalence" a relationship exists between the
__________ and _______ and the______. - answersquare of the ring AND diameter
AND THE antigen concentration.
By measuring the ring diameters produced by a number of samples of KNOWN
CONCENTRATION, a ________ can be constructed. - answercalibration curve
Measuring the ring diameter produced by a sample and reading off a CALIBRATION
CURVE may then determine the ____________ in an UNKNOWN sample. -
answerconcentration of the antigen igG.
Precipitation involves combination of _________ with ______ to produce ______ that
are visible. - answersoluble antigen WITH soluble antibody TO PRODUCE insoluble
complexes.
Agglutination, the technique used to identify ______ involves ________ that aggregate
to form larger complexes. - answerred cell antigens ; particulate antigens
_______ methods are commonly used in the laboratory - answerprecipitation
Procedures that are automated and results can be determined in less time. -
answerNephelometry and Turbimetry
, _________ has largely replaced RID in determining_______. - answerImmunofixation
electrophoresis ; serum immunoglobulin levels
RID. Replace lid and incubate at _________ a minimum of _______. - answerroom
temperature; 48-72 hours.
Measure the ring diameter tot he nearest ________. Record each value. -
answer0.1mm
_______ the value of the ring diameters for the ______. - answerSquare ; calibrators
Use the graphing paper to plot the ________. - answercalibrator values
The ______ should be along the horizontal X axis and the ______ should be along the
vertical Y axis. - answerimmunoglobulin concentrations ; ring diameters
The line of best fit is drawn through these _______. - answer3 points
Determine the concentration of the _______ in each test sample by first ________. -
answerimmunoglobulin; squaring the ring diameter
Find the value of the ______ on the Y axis then draw a line to the _______ then down
to the X axis to find the sample ___________. - answerdiameter; calibration curve;
concentration of the immunoglobulin.
Normal range of igG: _________ mg/dl _______ IU/ml - answer700-1700 ; 80-195
In normal adults, igG constituents approximately _____ of the total _______. -
answer75% ; serum immunoglobulins
The majority of igG is synthesized in the _____. - answerplasma cells
igG can pass the ______ and is critical for the ______ protection until its immune
system develops. - answerplacenta ; neonatal
Serum igG levels are associated with ________ and ______. - answerchronic liver
disease and myeloma (cancer of plasma cells)
Reduced levels may be associated with ________ and other severe _______. -
answermalignancy; severe protein-losing conditions
In this technique, where is the antigen and where is the antibody? - answerantigen= igG
placed in wells
antibody= anti-igG in gel
