MB ASCP EXAM 2026 CERTIFICATION
EVALUATION PAPER QUESTIONS WITH
ACCURATE SOLUTIONS
◉ Increasing Temperature increases or decreases stringency?
Answer: Increases
◉ Increase probe length increases or decreases stringency? Answer:
Increases
◉ increase probe complexity increases or decreases stringency?
Answer: Increases
◉ lower salt concentration increases or decreases stringency?
Answer: Increases
◉ Less time increases or decreases stringency? Answer: Increases
◉ adding formamide increases or decreases stringency? Answer:
Decreases
◉ adding bovine serum increases or decreases stringency? Answer:
Decreases
,◉ Increasing probe concentration increases or decreases
stringency? Answer: Decreases
◉ PCR Inhibitors Answer: Hemoglobin, Heparin, Xylene, Pheonl,
Cyanol, and Bromophenal blue
◉ Preferred Tubes for molecular testing? Answer: Lavender(EDTA)
and Yellow(ACD=Acid Citrate Dextrose)
◉ Specialized tubes for molecular testing? Answer: PPT=Plasma
Preparation Tube- designed to separate lymphocytes/granulocytes
for ease of DNA isolation extractions
◉ CML Translocation & Method of Detection? Answer: 11:22 or 9:22,
Fish/Cish
◉ Philedelphia chromosome disorder? Answer: CML
◉ Ewings Sarcoma (Solid Tumor) Translocation & Method of
detection? Answer: 11:22, RTPCR
◉ ALL Translocation & Method of detection? Answer: 9:22,
FISH/CISH:/RTPCR
, ◉ Follicular Lymphoma Translocation & Method of detection?
Answer: 14:18, FISH/RTPCR
◉ PML Translocation & Method of detection? Answer: 15:17, FISH/
RTPCR
◉ Mantle Cell Lymphoma Translocation & Method of detection?
Answer: 11:14, FISH/PCR/RT-PCR
◉ Burkitt Lymphoma Translocation & Method of detection? Answer:
8:14,FISH/CISH
◉ Rhabdomyosarcoma (RMS/ARMS)Translocation & Method of
detection? Answer: 2:13 or 1:13, RTPCR/PCR/ FISH
◉ HER2/neu Breast Cancer Chromosome & method of Detection?
Answer: 17, IHC:FISH:CISH
◉ Tumor Protein 53 Chromosome & method of Detection? Answer:
17, SSCP: DS
◉ BRCA1/2 Breast Cancer Chromosome & method of Detection?
Answer: 17/13, SSCP: DS
EVALUATION PAPER QUESTIONS WITH
ACCURATE SOLUTIONS
◉ Increasing Temperature increases or decreases stringency?
Answer: Increases
◉ Increase probe length increases or decreases stringency? Answer:
Increases
◉ increase probe complexity increases or decreases stringency?
Answer: Increases
◉ lower salt concentration increases or decreases stringency?
Answer: Increases
◉ Less time increases or decreases stringency? Answer: Increases
◉ adding formamide increases or decreases stringency? Answer:
Decreases
◉ adding bovine serum increases or decreases stringency? Answer:
Decreases
,◉ Increasing probe concentration increases or decreases
stringency? Answer: Decreases
◉ PCR Inhibitors Answer: Hemoglobin, Heparin, Xylene, Pheonl,
Cyanol, and Bromophenal blue
◉ Preferred Tubes for molecular testing? Answer: Lavender(EDTA)
and Yellow(ACD=Acid Citrate Dextrose)
◉ Specialized tubes for molecular testing? Answer: PPT=Plasma
Preparation Tube- designed to separate lymphocytes/granulocytes
for ease of DNA isolation extractions
◉ CML Translocation & Method of Detection? Answer: 11:22 or 9:22,
Fish/Cish
◉ Philedelphia chromosome disorder? Answer: CML
◉ Ewings Sarcoma (Solid Tumor) Translocation & Method of
detection? Answer: 11:22, RTPCR
◉ ALL Translocation & Method of detection? Answer: 9:22,
FISH/CISH:/RTPCR
, ◉ Follicular Lymphoma Translocation & Method of detection?
Answer: 14:18, FISH/RTPCR
◉ PML Translocation & Method of detection? Answer: 15:17, FISH/
RTPCR
◉ Mantle Cell Lymphoma Translocation & Method of detection?
Answer: 11:14, FISH/PCR/RT-PCR
◉ Burkitt Lymphoma Translocation & Method of detection? Answer:
8:14,FISH/CISH
◉ Rhabdomyosarcoma (RMS/ARMS)Translocation & Method of
detection? Answer: 2:13 or 1:13, RTPCR/PCR/ FISH
◉ HER2/neu Breast Cancer Chromosome & method of Detection?
Answer: 17, IHC:FISH:CISH
◉ Tumor Protein 53 Chromosome & method of Detection? Answer:
17, SSCP: DS
◉ BRCA1/2 Breast Cancer Chromosome & method of Detection?
Answer: 17/13, SSCP: DS
