Chapter 23 chromatography Mobile Phase
Chromatogram Flow
chromatography rate speed
:
column of the
u
Cluent mobile mobile phase
:
phase & mixture
passing
↓ S M
Em tri trz
through a column
column : mL/min
stationary phase retention time
* volume
↓ * linear cm/min
>
eluate : mobile & mixture
phase components
retention allows for determination
depends upon
1) size of
analyte
2) for SP tm dead time
=
affinity unretained components
rate of
3) temperature - = retention time component determine
migration
4) pH t =
adjusted retention
5) pressure time
(tr-ty)
6) Flow rate
7) diameter of SP
retention factor
wet
t & related
ratio of
8) column diameter of volume of SP
the time that
up
the solute spends in the
relative retention/selectivity factor
s
stationary phase to w =
W
&
awas
= t P
-
the time it spends in
mobile *
no separation the phase solve for
top
* * determine
used to determine the resolving power
if w= tr
of a column
0 =
+m affinity of
analyte
if w'll too fast to SP
/good Mead ! !
-
if k's 20 too slow
: broad bands -
Separation Efficiency
Is
good !
if I
1) differences in retention time
2) peak widths
measuring breadth
# of theoretical plates # of
Will width at half height
times a solute partitions between W width at the between
baseline
2
Fe
MP & Sp4NA N = S SY.
tangents drawn to the steepest parts
HETP = L of the
-
or peam
(height) ↓ N
16(z)2 Der =or t
~ =
resolution =
A
A * >1 5 A .
W
H = A +
B + Cu
van Deemter desired
W
*
Chromatogram Flow
chromatography rate speed
:
column of the
u
Cluent mobile mobile phase
:
phase & mixture
passing
↓ S M
Em tri trz
through a column
column : mL/min
stationary phase retention time
* volume
↓ * linear cm/min
>
eluate : mobile & mixture
phase components
retention allows for determination
depends upon
1) size of
analyte
2) for SP tm dead time
=
affinity unretained components
rate of
3) temperature - = retention time component determine
migration
4) pH t =
adjusted retention
5) pressure time
(tr-ty)
6) Flow rate
7) diameter of SP
retention factor
wet
t & related
ratio of
8) column diameter of volume of SP
the time that
up
the solute spends in the
relative retention/selectivity factor
s
stationary phase to w =
W
&
awas
= t P
-
the time it spends in
mobile *
no separation the phase solve for
top
* * determine
used to determine the resolving power
if w= tr
of a column
0 =
+m affinity of
analyte
if w'll too fast to SP
/good Mead ! !
-
if k's 20 too slow
: broad bands -
Separation Efficiency
Is
good !
if I
1) differences in retention time
2) peak widths
measuring breadth
# of theoretical plates # of
Will width at half height
times a solute partitions between W width at the between
baseline
2
Fe
MP & Sp4NA N = S SY.
tangents drawn to the steepest parts
HETP = L of the
-
or peam
(height) ↓ N
16(z)2 Der =or t
~ =
resolution =
A
A * >1 5 A .
W
H = A +
B + Cu
van Deemter desired
W
*
