MIC 205 LAB FINAL EXAM QUESTIONS & ANSWERS
What are microbes? - Answers -microbes are living things (or viruses) that are too
small to see by eye
*many microbes growing together can be visible
What is the smallest object that an unaided human eye can see? - Answers -0.1 mm
long or 100µm
Under the right conditions the human eye could see.... - Answers -- an amoeba proteus
- a human egg
- a paramecium
Would you be surprised to find microbes on vegetables at a grocery store? - Answers -
Yes, we don't usually see microbes. They are too small for the unaided eye.
Where can microbes be found? - Answers -everywhere
most healthy humans have more than 1000 different microbes on their skin - Answers -
corynebacterium striatum bacteria are some of the most common skin microbes
What are the main groups of microbes? - Answers -- viruses
- protozoa
- gram positive bacteria
- gram negative bacteria
- fungi
2 microbes that do NOT cause disease - Answers -- Prevotella spp. bacteria (found in
the intestines of healthy cats)
- Rhizobium leguminosarum bacteria (help plants get nutrients)
2 microbes that cause disease - Answers -- Aspergillus fumigatus or influenza virus
- Clostridioides difficile bacteria
which group(s) of microbes have complex cells? - Answers -- protozoa
- fungi
which group(s) of microbes have proteins? - Answers -- viruses
- gram neg. bacteria
- gram + bacteria
- fungi
- protozoa
which group(s) of microbes have some members with NO DNA? - Answers -- viruses
, which group(s) of microbes are often stained with LPBC before viewing under the
microscope? - Answers -- fungi
Viruses - Answers -- not a cell type
- can have DNA, RNA, and proteins
- no DNA
Gram + bacteria - Answers -- simple, one membrane
- DNA, RNA, proteins
Gram - bacteria - Answers -- simple, two membranes
- DNA, RNA, proteins
Fungi - Answers -- complex: cell membrane & organelles
- DNA, RNA, proteins
Protozoa - Answers -- complex: cell membrane & organelles
- DNA, RNA, proteins
Which group of microbes has simple cells with 2 membranes? - Answers -Gram
negative bacteria
which group of microbes does not have cells? - Answers -viruses
What types of microscopy have we learned in class? - Answers -- TEM
- Light microscopy
- Fluorescence microscopy
- SEM
In general, how do the bacteria look under light microscopy without staining? - Answers
-they look like nothing
Light microscopy - Answers -shows microbes in color (gram stain)
fluroescence microscopy - Answers -methods of characterization of lipid membranes:
uses fluorescence and phosphorescence instead of, or in addition to, reflection and
absorption to study properties of organic or inorganic substances
Scanning Electron Microscope (SEM) - Answers -displays black & white image
- show bacterial surface at high magnification
- is 3D outline of microbe
Transmission Electron Microscope (TEM) - Answers -Black & White image (can see
microbes more detailed)
What are microbes? - Answers -microbes are living things (or viruses) that are too
small to see by eye
*many microbes growing together can be visible
What is the smallest object that an unaided human eye can see? - Answers -0.1 mm
long or 100µm
Under the right conditions the human eye could see.... - Answers -- an amoeba proteus
- a human egg
- a paramecium
Would you be surprised to find microbes on vegetables at a grocery store? - Answers -
Yes, we don't usually see microbes. They are too small for the unaided eye.
Where can microbes be found? - Answers -everywhere
most healthy humans have more than 1000 different microbes on their skin - Answers -
corynebacterium striatum bacteria are some of the most common skin microbes
What are the main groups of microbes? - Answers -- viruses
- protozoa
- gram positive bacteria
- gram negative bacteria
- fungi
2 microbes that do NOT cause disease - Answers -- Prevotella spp. bacteria (found in
the intestines of healthy cats)
- Rhizobium leguminosarum bacteria (help plants get nutrients)
2 microbes that cause disease - Answers -- Aspergillus fumigatus or influenza virus
- Clostridioides difficile bacteria
which group(s) of microbes have complex cells? - Answers -- protozoa
- fungi
which group(s) of microbes have proteins? - Answers -- viruses
- gram neg. bacteria
- gram + bacteria
- fungi
- protozoa
which group(s) of microbes have some members with NO DNA? - Answers -- viruses
, which group(s) of microbes are often stained with LPBC before viewing under the
microscope? - Answers -- fungi
Viruses - Answers -- not a cell type
- can have DNA, RNA, and proteins
- no DNA
Gram + bacteria - Answers -- simple, one membrane
- DNA, RNA, proteins
Gram - bacteria - Answers -- simple, two membranes
- DNA, RNA, proteins
Fungi - Answers -- complex: cell membrane & organelles
- DNA, RNA, proteins
Protozoa - Answers -- complex: cell membrane & organelles
- DNA, RNA, proteins
Which group of microbes has simple cells with 2 membranes? - Answers -Gram
negative bacteria
which group of microbes does not have cells? - Answers -viruses
What types of microscopy have we learned in class? - Answers -- TEM
- Light microscopy
- Fluorescence microscopy
- SEM
In general, how do the bacteria look under light microscopy without staining? - Answers
-they look like nothing
Light microscopy - Answers -shows microbes in color (gram stain)
fluroescence microscopy - Answers -methods of characterization of lipid membranes:
uses fluorescence and phosphorescence instead of, or in addition to, reflection and
absorption to study properties of organic or inorganic substances
Scanning Electron Microscope (SEM) - Answers -displays black & white image
- show bacterial surface at high magnification
- is 3D outline of microbe
Transmission Electron Microscope (TEM) - Answers -Black & White image (can see
microbes more detailed)
