BIOL 2083, Section 1
Final Exam
Name (PLEASE PRINT CLEARLY)
Answer the questions completely and legibly using only the space provided.
True/False (1 pt each)
1. _T The hydrogens in water molecules have a partial positive charge.
2. _T The folded state of a protein is its lowest energy state.
3. T Enzymes lower the activation free energy ΔG‡ for chemical reactions.
4. _T The gluconeogenesis pathway uses ATP, but glycolysis produces ATP.
5. _T Glucose is added to Hemoglobin A1C nonenzymatically.
6. _T The transfer of electrons from water to NADP+ is not spontaneous.
7. Which of the following is true of the buffer MES (pKa 6.1)? (4 pts)
A. It is completely deprotonated at p H 8.8.
B. It is completely protonated at pH 6.3.
C. It is will be useful for maintaining a solution at pH 9.5.
D. All of the above
8. Which of the following amino acids has a side chain that would form an attractive
electrostatic interaction with the side chain of phenylalanine at pH 7? (4 pts)
A. Tyrosine
B. Cysteine
C. Lysine
D. The side chain of phenylalanine cannot participate in electrostatic interactions.
9. Which of the following is true regarding protein structure? (4 pts)
A. Peptide bonds are noncovalent bonds between the alpha carbon and the amide
nitrogen.
B. Disulfide bonds are formed between the side chains of methionine residues.
C. There is free rotation around Phi bonds, but it is limited by steric hindrance.
D. All of the above
, 10. Which of the following techniques would be most useful for separating protein A from
the mixture described below? (4 pts)
Protein A: 80 kDa monomer, pI = 6
Protein B: A dimer of 40 kDa subunits, pI = 7
Protein C: A dimer of 20 kDa subunits, pI = 8; binds glucose
A. Affinity chromatography
B. Ion exchange chromatography
C. Gel filtration chromatography
D. Any of these
11. Which of the following is true regarding a Lineweaver-Burk plot? (4 pts)
A. For Michaelis-Menten enzymes, it is a rectangular hyperbola.
B. The y-axis is 1/(substrate concentration).
C. The x-intercept is -1/Km.
D. All of these
12. Which of the following is true of the T/R forms and models of allostery? (4 pts)
A. In the concerted model, T R conversion occurs spontaneously.
B. In the sequential model, R state is less active but more stable vs. the T state.
C. In the concerted model, T/R hybrids are allowed.
D. All of the above
13. In the absence of an inhibitor, the Vmax of the enzyme is 1400nmol product/min and the
Km is 0.2mM. In the presence of the inhibitor, the Vmax is 1400nmol product/min and
the Km is 20mM. What type of inhibition is occurring? (4 pts)
A. Uncompetitive
B. Noncompetitive
C. Competitive
D. Note enough information to determine the type of inhibition.
14. Which of the following is true of normal, adult hemoglobin? (4 pts)
A. 2,3 BPG is a positive allosteric effector that favors the R state.
B. In the T state the Fe atom is just outside of the plane of the heme ring.
C. Carbon dioxide binds in a basic pocket at the dimer-dimer interface.
D. All of the above
15. Which of the following is true regarding phospholipids and biological membranes? (4
pts)
A. The presence of lipids with fatty acids containing cis unsaturation will decrease
fluidity.
B. The presence of lipids with shorter chain fatty acids will decrease the melting
temperature.
C. The fatty acid tail groups on lipids orient away from each other and toward
aqueous solution.
D. All of the above
Final Exam
Name (PLEASE PRINT CLEARLY)
Answer the questions completely and legibly using only the space provided.
True/False (1 pt each)
1. _T The hydrogens in water molecules have a partial positive charge.
2. _T The folded state of a protein is its lowest energy state.
3. T Enzymes lower the activation free energy ΔG‡ for chemical reactions.
4. _T The gluconeogenesis pathway uses ATP, but glycolysis produces ATP.
5. _T Glucose is added to Hemoglobin A1C nonenzymatically.
6. _T The transfer of electrons from water to NADP+ is not spontaneous.
7. Which of the following is true of the buffer MES (pKa 6.1)? (4 pts)
A. It is completely deprotonated at p H 8.8.
B. It is completely protonated at pH 6.3.
C. It is will be useful for maintaining a solution at pH 9.5.
D. All of the above
8. Which of the following amino acids has a side chain that would form an attractive
electrostatic interaction with the side chain of phenylalanine at pH 7? (4 pts)
A. Tyrosine
B. Cysteine
C. Lysine
D. The side chain of phenylalanine cannot participate in electrostatic interactions.
9. Which of the following is true regarding protein structure? (4 pts)
A. Peptide bonds are noncovalent bonds between the alpha carbon and the amide
nitrogen.
B. Disulfide bonds are formed between the side chains of methionine residues.
C. There is free rotation around Phi bonds, but it is limited by steric hindrance.
D. All of the above
, 10. Which of the following techniques would be most useful for separating protein A from
the mixture described below? (4 pts)
Protein A: 80 kDa monomer, pI = 6
Protein B: A dimer of 40 kDa subunits, pI = 7
Protein C: A dimer of 20 kDa subunits, pI = 8; binds glucose
A. Affinity chromatography
B. Ion exchange chromatography
C. Gel filtration chromatography
D. Any of these
11. Which of the following is true regarding a Lineweaver-Burk plot? (4 pts)
A. For Michaelis-Menten enzymes, it is a rectangular hyperbola.
B. The y-axis is 1/(substrate concentration).
C. The x-intercept is -1/Km.
D. All of these
12. Which of the following is true of the T/R forms and models of allostery? (4 pts)
A. In the concerted model, T R conversion occurs spontaneously.
B. In the sequential model, R state is less active but more stable vs. the T state.
C. In the concerted model, T/R hybrids are allowed.
D. All of the above
13. In the absence of an inhibitor, the Vmax of the enzyme is 1400nmol product/min and the
Km is 0.2mM. In the presence of the inhibitor, the Vmax is 1400nmol product/min and
the Km is 20mM. What type of inhibition is occurring? (4 pts)
A. Uncompetitive
B. Noncompetitive
C. Competitive
D. Note enough information to determine the type of inhibition.
14. Which of the following is true of normal, adult hemoglobin? (4 pts)
A. 2,3 BPG is a positive allosteric effector that favors the R state.
B. In the T state the Fe atom is just outside of the plane of the heme ring.
C. Carbon dioxide binds in a basic pocket at the dimer-dimer interface.
D. All of the above
15. Which of the following is true regarding phospholipids and biological membranes? (4
pts)
A. The presence of lipids with fatty acids containing cis unsaturation will decrease
fluidity.
B. The presence of lipids with shorter chain fatty acids will decrease the melting
temperature.
C. The fatty acid tail groups on lipids orient away from each other and toward
aqueous solution.
D. All of the above
