BIO 340 UNIT 1 EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
______ sequence near hairpin destabilizes base pairing between
RNA transcript and DNA template; they dissociate, ending
transcription - correct answer- U-rich
_______ not only replicate DNA but also proof-read newly
synthesized DNA for accuracy - correct answer- DNA
polymerases
____-to-______ direction of growing RNA strand chain growth
- correct answer- 5';3'
1st generation DNA sequencing methods - correct answer-
1. Dideoxynucleotide sequencing
2. Manual sequencing
3. Automated sequencing
1st generation method of DNA sequencing - correct answer-
dideoxy "chain termination" sequencing
,3rd generation DNA sequencing - correct answer- Nanopore
sequencing
A ribonucleoprotein that synthesizes telomeric repeat
sequences to maintain telomere length in germ-line and stem
cells - correct answer- Telomerase
After __ PCR cycles, the target sequence defined by the primers
begins to accumulate. - correct answer- 3
After 30 cycles of PCR how many copies of the target sequence
are produced from a single starting molecule? - correct
answer- As many as a billion
Allows for looping out and excision of intron at 5' splice junction;
also juxtaposes exon ends for splicing - correct answer-
Branch point A
Allows RNA polymerase to exhibit several unique differences
from DNA polymerase - correct answer- Mg2+ catalyzed
synthesis reaction
, Are poly(a) tails resistant to degradation by cytosolic
exonucleases? - correct answer- No
At a slightly higher temperature, the enzyme taq polymerase
binds to the prime sequences and adds nucleotides to extend
the second strand. This completes the first cycle. - correct
answer- Third step of PCR
Binds promoter sequences at two sites - correct answer-
Sigma factor
Catalyzed by Mg2+ ion in core enzyme - correct answer-
Nucleoside triphosphate hydrolysis
Code characteristics - correct answer- Triplet code,
universality, degeneracy, redundancy
Coding sequences utilized for translation - correct answer-
Exons
Complementary base pairs consist of a ______ and a _________
- correct answer- Purine; pyrimidine
DETAILED ANSWERS (VERIFIED ANSWERS)
______ sequence near hairpin destabilizes base pairing between
RNA transcript and DNA template; they dissociate, ending
transcription - correct answer- U-rich
_______ not only replicate DNA but also proof-read newly
synthesized DNA for accuracy - correct answer- DNA
polymerases
____-to-______ direction of growing RNA strand chain growth
- correct answer- 5';3'
1st generation DNA sequencing methods - correct answer-
1. Dideoxynucleotide sequencing
2. Manual sequencing
3. Automated sequencing
1st generation method of DNA sequencing - correct answer-
dideoxy "chain termination" sequencing
,3rd generation DNA sequencing - correct answer- Nanopore
sequencing
A ribonucleoprotein that synthesizes telomeric repeat
sequences to maintain telomere length in germ-line and stem
cells - correct answer- Telomerase
After __ PCR cycles, the target sequence defined by the primers
begins to accumulate. - correct answer- 3
After 30 cycles of PCR how many copies of the target sequence
are produced from a single starting molecule? - correct
answer- As many as a billion
Allows for looping out and excision of intron at 5' splice junction;
also juxtaposes exon ends for splicing - correct answer-
Branch point A
Allows RNA polymerase to exhibit several unique differences
from DNA polymerase - correct answer- Mg2+ catalyzed
synthesis reaction
, Are poly(a) tails resistant to degradation by cytosolic
exonucleases? - correct answer- No
At a slightly higher temperature, the enzyme taq polymerase
binds to the prime sequences and adds nucleotides to extend
the second strand. This completes the first cycle. - correct
answer- Third step of PCR
Binds promoter sequences at two sites - correct answer-
Sigma factor
Catalyzed by Mg2+ ion in core enzyme - correct answer-
Nucleoside triphosphate hydrolysis
Code characteristics - correct answer- Triplet code,
universality, degeneracy, redundancy
Coding sequences utilized for translation - correct answer-
Exons
Complementary base pairs consist of a ______ and a _________
- correct answer- Purine; pyrimidine
