Protospacer Study guides, Study notes & Summaries

CRISPR Cas-9 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Describe i...

HC 7- DNA Pair & Genome Editing Questions & Answers 1 / 3 1. What are causes for gene knockout? Non-homologous end joining 2. What is gene knockout? premature stop coden: when the function of a gene has been disrupted or turned off to study gene function 3. What are repair mechanism for DSB? Non-homologeus end joint (NHEJ) + Homologues recombination 4. Where stands CRISPR for? Clustered Regularly Interspaced Short Palindromic Repeats 5. Working mechanism of CRISPR? he palindromic ...

HC 7- DNA Pair & Genome Editing Questions & Answers 1 / 3 1. What are causes for gene knockout? Non-homologous end joining 2. What is gene knockout? premature stop coden: when the function of a gene has been disrupted or turned off to study gene function 3. What are repair mechanism for DSB? Non-homologeus end joint (NHEJ) + Homologues recombination 4. Where stands CRISPR for? Clustered Regularly Interspaced Short Palindromic Repeats 5. Working mechanism of CRISPR? he palindromic ...

CRISPR Cas-9 Semester 1 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM De...

CRISPR Cas Written Exam Questions With 100% Correct Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Descr...

Molecular Diagnostics Chapter 1, questions and answers with rated solutions trait particular characteristic Which elements make up the macromolecule of DNA? carbon, nitrogen, oxygen, phosphorous, and hydrogen What is the structure of DNA? phosphorylated ribose sugar (deoxy ribose) backbone with a nitrogenous base (guanine, adenine, cytosine, thymine) two strands joined together through hydrogen bonds form a double helix What type of bond joins two nucleotides? phosphodie...

A comprehensive MindMap summarising the CRISPR/Cas gene editing system.

(chapter5) Microbiology Test 2 1. Which growth phase has the largest increase in cell numbers per unit of time? death phase exponential phase lag phase stationary phase In which growth p hase is there no increase or decrease in cell number because some cells are dividing while others are dying? death phase exponential phase lag phase stationary phase To speed up the growth rate in a chemostat, the should be increased. To increase the cell density as well, the should be increased. A. dilution rat...

Summarised lecture notes outlining the CRISPR/Cas system. Contents can be seen on the first page.