Cloning vectors Study guides, Study notes & Summaries

Biotechnology |Cloning Vectors Questions and Answers

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

immunological assay method correct answers What method do we use when we want to find a protein we are interested in but we don't know the exact genetic sequence? antibody binding correct answers What mechanism does the immunological assay method use to highlight the desired protein? primary antibody correct answers In the immunological assay method, the ____ binds to the target protein. secondary antibody correct answers In the immunological assay method, the ____ reacts with the ...

MCB Exam 3 Questions & Answers 2023/2024 What is meant by recombinant DNA? - ANSWER-DNA w/fragments from 2+ sources Name the tools used to make a recombinant DNA. (mechanisms of gene cloning) - ANSWER-1) Isolate plasmid DNA from bacterial cell 2) Isolate DNA of interest 3) Insert gene of interest into plasmid DNA 4) Insert now recombinant DNA into bacterium 5) Culture bacteria to make copies Restriction enzymes - ANSWER-endonuclease that cuts DNA at specific sites Cloning vecto...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

If a foreign gene is cloned into an expression host, it is important that the host itself A) not produce the protein being studied. B) produce the protein in larger amounts than the vector. C) repress the genetic expression being studied. D) produce signal proteins to tag the host protein. A Detecting a specific protein with an antibody is considered a(n) ________ method. A) selection B) screening C) isolation D) duplication B 00:02 01:22 To discover a catabolic...