Enzymology Study guides, Study notes & Summaries

Which of these statements is correct? A) A catalyst decreases the activation barrier by different amounts for the forward and reverse reactions. B) A catalyst decreases the activation barrier by the same amount for both the forward and reverse reactions. C) A catalyst increases the activation barrier by different amounts for the forward and reverse reactions. D) A catalyst increases the activation barrier by the same amount for both the forward and reverse reactions. E) A catalyst does ...

Physiology - - The study of function Homeostasis - - maintenance of the same state - maintaining order - requires energy and a means to utilized the energy Glucose Fatty Acids Amino Acids Volatile Fatty Acids Ketone - - Sources of energy

enzymes increase reactions by lowering the activation energy-which is the energy needed to go from the reactant to the transition state. The transition state is called the enzyme substrate complex - function of enzymes Classes of enzymes: oxidoreductase-oxidation/reduction reactions, transferase-group transfer reaction, hydrolase-hydrolytic reactions, lyase- cleavage of C-C C-S C-N bonds, isomerase- isomerization, ligase-condensation of two molecules coupled with cleavage of ATP. - class of...

Enzyme - A PROTEIN that acts as a biochemical CATALYST by affecting the rate at which a reaction proceeds. Substrate - The material acted on by the enzyme Product - The material produced as the result of an enzyme acting upon a substrate. 1. Enzyme binds to substrate at their active site 2. [ES] complex brings substrate molecule into proper alignment with enzyme 3. Products are formed and the free enzyme can combine with excess free substrate 4. The enzyme is not altered or consumed in th...

Adaptability of Cells - specific proteins present and functioning at one given time are replaced or joined by new proteins. Lab 5 reaction Enzymology - PNPP + H2O -------------> PNP + Pi (colorless) (yellow) substrate Lab five Spectrometer set at what nm? - Make sure spectrophotometer is set to read absorbance at a wavelength of 410 nm. Change in OD = - 15 minute reading - zero minute reading Cellular respiration - The process that converts the energy in food molecules into the energ...

What is dogma in enzymology? - One reaction only has one enzyme to catalyse it What happens during a chemical reaction? - Activation energy provides enough energy for the process to happen where the reactants break or form bonds and create a new product What is transition state? - A less stable state that the products have to go through to get to the final product

Active Site - Attachment of substrate Often a water-free cavity Pouch-like Substrate - Substance acted upon enzyme Also called as chemical reactant Allosteric Site - Another cavity, other molecule other than substrate (inhibitors)

An enzyme maybe defined as a: - Biological catalysts The Michaelis-Menten Constant in the rate of conversion of substrate to product is determined by: - Substrate concentration and rate of dissociation of enzyme-substrate complex Isoenzyme - The electrophoretic property is different for each isoenzyme Isoenzymes are performed to improve - specificity The protein portion of an enzyme complex is called - Apoenzyme

A summary of the reader of the course Enzymology, BIC20806, at WUR

Summary of the 'biocatalysis' component within the course 'Enzymology BIC20806'.