6.5 Cultivation and enumeration of
viruses
1. List the types of approaches used to cultivate viruses,
noting which types of viruses are cultivated by each
method.
2. Describe three direct counting methods and two
indirect counting methods used to enumerate viruses.
3. Outline the events that lead to the formation of a
plaque in a lawn of bacterial cells.
4. Distinguish lethal dose from infectious dose.
40
, The Cultivation of Viruses
• Requires inoculation of appropriate living host
41
viruses
1. List the types of approaches used to cultivate viruses,
noting which types of viruses are cultivated by each
method.
2. Describe three direct counting methods and two
indirect counting methods used to enumerate viruses.
3. Outline the events that lead to the formation of a
plaque in a lawn of bacterial cells.
4. Distinguish lethal dose from infectious dose.
40
, The Cultivation of Viruses
• Requires inoculation of appropriate living host
41
