Knockout Mice - to study gene function
Making a GM mouse:
- To study gene function
- What happens to the phenotype of the
mouse if a gene is deleted
- What does this tell us about the gene’s
function
- The Nobel Prize in Physiology or
Medicine 2007:
How to make a GM mouse:
Combining advances in several different
area of biology:
- In vitro fertilisation
- Stem Cell biology
- Recombinant DNA technology/genetic
engineering or gene editing
- Sequencing technology
Homologous recombination:
- Exchange of genetic code between
identical/similar sequences
- A natural DNA repair mechanism
- Can be used to insert desired DNA
sequences
To make a GM mouse:
- Design a targeting vector (molecular
cloning)
- Homologous recombination in ES cells
- Screen ES cells
- Inject ES cells into blastocyst
- Implant in female mouse
- Breed GM offspring to generate homozygous mice
https://mmutube.mmu.ac.uk/media/TAIBMS%20-%20KO%20Mice/1_nqkktwrk
Making a GM mouse:
- To study gene function
- What happens to the phenotype of the
mouse if a gene is deleted
- What does this tell us about the gene’s
function
- The Nobel Prize in Physiology or
Medicine 2007:
How to make a GM mouse:
Combining advances in several different
area of biology:
- In vitro fertilisation
- Stem Cell biology
- Recombinant DNA technology/genetic
engineering or gene editing
- Sequencing technology
Homologous recombination:
- Exchange of genetic code between
identical/similar sequences
- A natural DNA repair mechanism
- Can be used to insert desired DNA
sequences
To make a GM mouse:
- Design a targeting vector (molecular
cloning)
- Homologous recombination in ES cells
- Screen ES cells
- Inject ES cells into blastocyst
- Implant in female mouse
- Breed GM offspring to generate homozygous mice
https://mmutube.mmu.ac.uk/media/TAIBMS%20-%20KO%20Mice/1_nqkktwrk
