BIOC1302 Experiments Biochemistry (BIOC1302)
University of Leeds (UoL)
Here are the best resources to pass BIOC1302 Experiments Biochemistry (BIOC1302). Find BIOC1302 Experiments Biochemistry (BIOC1302) study guides, notes, assignments, and much more.
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Answered Worksheet on Fluorescence assay
Measurement Of Serum Cholesterol Using A Fluorescence Assay
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Affinity Chromatography answered Worksheet
PopularTo purify α-lactalbumin from milk whey, using affinity chromatography. 
 
To identify the proteins in cow’s milk whey 
 
To determine the purity of α-lactalbumin separated from milk whey
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Molecular Exclusion Chromatography of Haemoglobin
Worksheets + Answers of Experiments performed during Year 1 Biochemistry. 
To separate molecules on the basis of size 
difference. 
Your learning 
objectives 
· To understand the practical and theoretical 
basis of molecular exclusion chromatography. 
· To understand how columns are set up and 
run. 
· To understand the terms 'bed volume' and 
'void volume' and how they are measured. 
· To be able to apply simple tests to identify 
the eluted proteins. 
· To appreciate some applicatio...
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Purifying proteins by ion exchange chromatography answered worksheet
Purifying proteins by ion exchange chromatography 
Aims of the experiment 
 	• To separate two proteins using difference in net charge. 
 	 
Your learning objectives	• To understand the practical and theoretical basis of ion exchange chromatography through its application to protein separation. 
• To understand how columns are set up and run. 
• To be able to use the knowledge to devise separation procedures using alternative ion exchange resins. 
• To appr...
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Thermodynamics of trypsin denaturation
To determine the activation energy for an enzyme catalysed reaction 
 
• To determine whether the thermal denaturation of trypsin is entropy or enthalpy driven
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Titration Curves of enzymes
How to make the titration curve of glycine and process data regarding this. Also showing principal concepts of: pH, PKa
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The use of buffers answered worksheet
Understanding the use of buffers in Biological sciences, answered worksheet (pH...etc)