CHEME 3320
Cornell University
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This is a comprehensive and detailed assignment n.o 4 for Cheme 3320. 
 
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CHEME 3320 Multi-Stage Extraction
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A mixture containing 7,000 kmol/hr of water and 3,000 ppm by weight of ammonia at 77 ºC and 1 atm is to be 
processed to remove 90% of ammonia. Some of properties of ammonia and water are listed below. 
component Mw Tf (ºC) Tb (ºC) Mol. Vol. δP δH pKa 
ammonia 17 -77 -33.4 20.8 13 11 4.75 
water 18 0 100 18.1 22.8 40.4 15.7
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CHEME 3320 Recitation 12
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o precipitate catalase from an enzyme mixture which includes cholesterol oxidase, our desired product. 
Catalase has a molecular weight of 250,000, a precipitate density of 1.3 g/cm3 
, an average diameter in solution of 10 
nm, and an average diffusion coefficient of 4.1 x 10-7 
cm2 
/sec. It is to be precipitated by a sudden pH change in a 
100 liter tank stirred with a 0.1 hp motor. The feed concentration is 0.2 g/liter and sticking coefficient is about 0.05 
sec. 
Assuming that nucleatio...
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CHEME 3320 Recitation 11
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Assuming that nucleation is fast and flocculation is negligible, and that the solution has properties close to those of 
water, estimate the following: 
a) the time over which diffusion will limit growth (i.e. time for homogeneity)
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CHEME 3320 Recitation 12
- Summary • 1 pages • 2023
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o precipitate catalase from an enzyme mixture which includes cholesterol oxidase, our desired product. 
Catalase has a molecular weight of 250,000, a precipitate density of 1.3 g/cm3 
, an average diameter in solution of 10 
nm, and an average diffusion coefficient of 4.1 x 10-7 
cm2 
/sec. It is to be precipitated by a sudden pH change in a 
100 liter tank stirred with a 0.1 hp motor. The feed concentration is 0.2 g/liter and sticking coefficient is about 0.05 
sec. 
Assuming that nucleatio...
In-depth analysis of the distillation process using multi-stage extraction and stripping methods
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