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BIO203 – Microscopy and Hair Analysis in Forensic Biology

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This comprehensive summary covers key concepts in microscopy and forensic hair analysis. It explains microscope parts and functions, magnification calculations, and proper focusing techniques. The notes detail the structure of hair (cuticle, cortex, medulla), medulla patterns, cuticle types, and differences between human and animal hair. It also includes growth phases, forensic applications, chemical analysis techniques, and DNA-based evidence considerations. Ideal for students in Forensic Science / Biology practical labs.

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BIO203 – Microscopy and Hair Analysis in Forensic
Biology
What is the magnification of the ocular lens? - 10X

What is the magnification of the low power objective? - 10X

High Power? - 40X

What is the total magnification of the ocular and low power? - 100X

Ocular and high power? - 400X

Which is the only adjustment knob you should use while on high power? - Fine
adjustment

Which adjustment knob do you use on low power? - Coarse and Fine adjustment

Light Source - Light built into the base (mirror or bulb)

Ocular Lens - Magnifies the image and focuses light into your eye

How do you calculate the power of magnification? - Multiply the power of the ocular lens by the
power of the objective.

Coarse Adjustment -

Fine Adjustment -

Objective -

Base and Arm - Structural support

Inclination Joint - located at the bottom of the instrument, where the base connects to the arm.
The inclination joint allows a microscope to tilt.

, Body Tube - The microscope body tube separates the objective and the eyepiece and assures
continuous alignment of the optics.

Revolving Nosepiece - This is the part that holds two or more objective lenses and can be rotated
to easily change power. Objective Lenses: Usually you will find 3 or 4 objective lenses on a
microscope.

Objective lens - Gathers light and magnifies image

High Power Objective - A high power objective lens is an important part of a microscope that
magnifies up to 40 times. This lens is used for observing fine details such as muscle striations
and retinal nerve cells.

Low Power Objective - The low-power objective on a microscope is the shortest or second
shortest objective lens found on standard laboratory microscopes. Low-power objective lenses
typically add 10x to the magnifying power of the microscope. Stage Clips - Holds slide

in place

Stage - Where slide is placed, holds slide

Diaphragm - Adjusting the amount of light striking an object.

Cuticle -

Cortex -

Follicle -

Root -

What are some things examining a hair sample can determine? - SOURCE (HUMAN OR
ANIMAL)

RACE (SOMETIMES)

ORIGIN OF THE LOCATION ON THE SOURCE'S BODY

SIGN OF STRUGGLE

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November 2, 2025
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