TEST BANK for Fundamentals of Molecular Virology 2nd
c c c c c c c
Edition by Acheson. c c c
Package Title: Testbank
c c
Course Title: Acheson 2nd edition
c c c c
Chapter Number: 01
c c c
Question type: Multiple Choice
c c c
1) Which of the following terms describes the protein shell that surrounds the viral genome?
c c c c c c c c c c c c c
a) capsid
b) envelope
c) matrix
d) virion
e) capsomere
Answer: a
c c
2) Which of the following would not be a nucleic acid form found in a viral genome?
c c c c c c c c c c c c c c c
a) dsDNA
b) ssDNA
c) dsRNA
d) ssRNA
e) an RNA:DNA hybrid
c c
c Answer: e c
3) Which of the following is an enzyme that most RNA viruses encode in their genome?
c c c c c c c c c c c c c c
a) DNA-dependent-RNA-polymerase
b) RNA-dependent-RNA-polymerase
c) DNA-dependent-DNA-polymerase
d) DNA-dependent-RNA-polymerase
e) RNA-dependent-protein-synthetase
Answer: b
c c
4) Viruses play a major role in the ecology of the ocean by doing which of the following?
c c c c c c c c c c c c c c c c
a) By infecting photosynthetic bacteria and increasing their growth.
c c c c c c c
,b) By infecting harmful bacteria found in the ocean and limiting their growth.
c c c c c c c c c c c
c) By killing off fish and preventing them from becoming too plentiful.
c c c c c c c c c c
d) By lysing unicellular organisms and releasing carbon and oxygen back into the ocean.
c c c c c c c c c c c c
e) All of the above statements are correct.
c c c c c c
Answer: d c
5) If you wanted to show that an infectious agent is a virus, what experiment could you do?
c c c c c c c c c c c c c c c c
a) Show that the agent can be seen under a light microscope.
c c c c c c c c c c
b) Show that agent contains a lipid membrane.
c c c c c c
c) Show that the agent can form single colonies on an agar plate.
c c c c c c c c c c c
d) Show that the agent can be diluted and can still cause disease in an animal.
c c c c c c c c c c c c c c
e) Show that the agent can pass through a porcelain filter and is still infectious.
c c c c c c c c c c c c c
Answer: e c
6) What does the word “virus” mean in Latin?
c c c c c c c
a) infectious agent c
b) liquid
c) poison
d) death
e) disease
Answer: c
c c
7) The word “phage” is a shortened version of the name of a virus that can infect which type of
c c c c c c c c c c c c c c c c c c
organism?
a) bacterial cell c
b) human cell c
c) eukaryotic cell c
d) plant cell c
e) inset cell c
c Answer: a c
8) The study of which group of viruses led to the discovery of cellular genes that can promoter cancer in
c c c c c c c c c c c c c c c c c c
animal cells?
c c
a) papillomaviruses
b) retroviruses
c) adneoviruses
d) polyomaviruses
,e) picornaviruses
Answer: b
c c
9) When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is
c c c c c c c c c c c c c c c c c c
put on top of the cells after they have been infected with the diluted virus. What is the major purpose of
c c c c c c c c c c c c c c c c c c c c c
this agar layer?
c c c
a) It helps to feed the cells as they grow.
c c c c c c c c
b) It promotes the replication of the virus.
c c c c c c
c) It makes the infected cells easier to visualize.
c c c c c c c
d) It limits the movement of virus particles.
c c c c c c
e) All of the above are correct.
c c c c c
Answer: d c
10) To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x
c c c c c c c c c c c c c c c c c c c c c c
107 pfu/ml onto a plate of animal cells that contains 5 x 106 cells. What is the multiplicity of infection for
c c c c c c c c c c c c c c c c c c c c c
this experiment?
c c
a) 12
b) 6
c) 5
d) 3
e) 1
c Answer:
b
c
11) During a single growth cycle experiment, the titer of extracellular and intracellular virus drops in the
c c c c c c c c c c c c c c c
first hour. What explains this observation?
c c c c c c
a) The virus is inactivated by antibodies in the medium.
c c c c c c c c
b) The virus is inactivated by cellular enzymes.
c c c c c c
c) The virus enters the cell and is uncoated.
c c c c c c c
d) The virus binds to the cells in the dish and can’t be released.
c c c c c c c c c c c c
e) The cell’s antiviral defenses prevent replication of the virus
c c c c c c c c
Answer: c c
12) What is another term for an RNA-dependent-DNA-polymerase?
c c c c c c
a) DNA polymerase c
b) RNA polymerase II c c
, c) RNA replicase c
d) RNA transcriptase c
e) Reverse transcriptase c
c Answer: e c
13) Plant viruses use which cellular structures to spread between host cells?
c c c c c c c c c c
a) endosomes
b) plasmodesmata
c) plasma membranes c
d) vesicles
e) receptors
Answer: b
c c
14) All viruses that use a negative-sense RNA genome must package which of the following proteins in
c c c c c c c c c c c c c c c
their virion?
c c
a) RNA-dependent-RNA-polymerase
b) matrix protein c
c) RNA helicase c
d) RNA methylase c
e) scaffolding protein c
c Answer: a c
15) Which of the following is a description of a plaque?
c c c c c c c c c
a) A viral particle as seen by electron microscopy.
c c c c c c c
b) A region of dead cells in a monolayer of infected cells.
c c c c c c c c c c
c) A skin lesion caused by a virus infection in an animal.
c c c c c c c c c c
d) A button of red blood cells seen in a hemagglutination assay.
c c c c c c c c c c
e) A region of crystallized virus particles in an infected cell.
c c c c c c c c c
Answer: b c
16) Which of the following processes in cells was NOT discovered by studying a virus?
c c c c c c c c c c c c c
a) the sequence of the genetic code.
c c c c c
b) DNA replication in both prokaryotic and eukaryotic cells
c c c c c c c
c) DNA is the genetic material
c c c c
d) regulation of gene expression in eukaryotic cells. c c c c c c
e) mRNA splicing in eukaryotic cells
c c c c
c c c c c c c
Edition by Acheson. c c c
Package Title: Testbank
c c
Course Title: Acheson 2nd edition
c c c c
Chapter Number: 01
c c c
Question type: Multiple Choice
c c c
1) Which of the following terms describes the protein shell that surrounds the viral genome?
c c c c c c c c c c c c c
a) capsid
b) envelope
c) matrix
d) virion
e) capsomere
Answer: a
c c
2) Which of the following would not be a nucleic acid form found in a viral genome?
c c c c c c c c c c c c c c c
a) dsDNA
b) ssDNA
c) dsRNA
d) ssRNA
e) an RNA:DNA hybrid
c c
c Answer: e c
3) Which of the following is an enzyme that most RNA viruses encode in their genome?
c c c c c c c c c c c c c c
a) DNA-dependent-RNA-polymerase
b) RNA-dependent-RNA-polymerase
c) DNA-dependent-DNA-polymerase
d) DNA-dependent-RNA-polymerase
e) RNA-dependent-protein-synthetase
Answer: b
c c
4) Viruses play a major role in the ecology of the ocean by doing which of the following?
c c c c c c c c c c c c c c c c
a) By infecting photosynthetic bacteria and increasing their growth.
c c c c c c c
,b) By infecting harmful bacteria found in the ocean and limiting their growth.
c c c c c c c c c c c
c) By killing off fish and preventing them from becoming too plentiful.
c c c c c c c c c c
d) By lysing unicellular organisms and releasing carbon and oxygen back into the ocean.
c c c c c c c c c c c c
e) All of the above statements are correct.
c c c c c c
Answer: d c
5) If you wanted to show that an infectious agent is a virus, what experiment could you do?
c c c c c c c c c c c c c c c c
a) Show that the agent can be seen under a light microscope.
c c c c c c c c c c
b) Show that agent contains a lipid membrane.
c c c c c c
c) Show that the agent can form single colonies on an agar plate.
c c c c c c c c c c c
d) Show that the agent can be diluted and can still cause disease in an animal.
c c c c c c c c c c c c c c
e) Show that the agent can pass through a porcelain filter and is still infectious.
c c c c c c c c c c c c c
Answer: e c
6) What does the word “virus” mean in Latin?
c c c c c c c
a) infectious agent c
b) liquid
c) poison
d) death
e) disease
Answer: c
c c
7) The word “phage” is a shortened version of the name of a virus that can infect which type of
c c c c c c c c c c c c c c c c c c
organism?
a) bacterial cell c
b) human cell c
c) eukaryotic cell c
d) plant cell c
e) inset cell c
c Answer: a c
8) The study of which group of viruses led to the discovery of cellular genes that can promoter cancer in
c c c c c c c c c c c c c c c c c c
animal cells?
c c
a) papillomaviruses
b) retroviruses
c) adneoviruses
d) polyomaviruses
,e) picornaviruses
Answer: b
c c
9) When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is
c c c c c c c c c c c c c c c c c c
put on top of the cells after they have been infected with the diluted virus. What is the major purpose of
c c c c c c c c c c c c c c c c c c c c c
this agar layer?
c c c
a) It helps to feed the cells as they grow.
c c c c c c c c
b) It promotes the replication of the virus.
c c c c c c
c) It makes the infected cells easier to visualize.
c c c c c c c
d) It limits the movement of virus particles.
c c c c c c
e) All of the above are correct.
c c c c c
Answer: d c
10) To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x
c c c c c c c c c c c c c c c c c c c c c c
107 pfu/ml onto a plate of animal cells that contains 5 x 106 cells. What is the multiplicity of infection for
c c c c c c c c c c c c c c c c c c c c c
this experiment?
c c
a) 12
b) 6
c) 5
d) 3
e) 1
c Answer:
b
c
11) During a single growth cycle experiment, the titer of extracellular and intracellular virus drops in the
c c c c c c c c c c c c c c c
first hour. What explains this observation?
c c c c c c
a) The virus is inactivated by antibodies in the medium.
c c c c c c c c
b) The virus is inactivated by cellular enzymes.
c c c c c c
c) The virus enters the cell and is uncoated.
c c c c c c c
d) The virus binds to the cells in the dish and can’t be released.
c c c c c c c c c c c c
e) The cell’s antiviral defenses prevent replication of the virus
c c c c c c c c
Answer: c c
12) What is another term for an RNA-dependent-DNA-polymerase?
c c c c c c
a) DNA polymerase c
b) RNA polymerase II c c
, c) RNA replicase c
d) RNA transcriptase c
e) Reverse transcriptase c
c Answer: e c
13) Plant viruses use which cellular structures to spread between host cells?
c c c c c c c c c c
a) endosomes
b) plasmodesmata
c) plasma membranes c
d) vesicles
e) receptors
Answer: b
c c
14) All viruses that use a negative-sense RNA genome must package which of the following proteins in
c c c c c c c c c c c c c c c
their virion?
c c
a) RNA-dependent-RNA-polymerase
b) matrix protein c
c) RNA helicase c
d) RNA methylase c
e) scaffolding protein c
c Answer: a c
15) Which of the following is a description of a plaque?
c c c c c c c c c
a) A viral particle as seen by electron microscopy.
c c c c c c c
b) A region of dead cells in a monolayer of infected cells.
c c c c c c c c c c
c) A skin lesion caused by a virus infection in an animal.
c c c c c c c c c c
d) A button of red blood cells seen in a hemagglutination assay.
c c c c c c c c c c
e) A region of crystallized virus particles in an infected cell.
c c c c c c c c c
Answer: b c
16) Which of the following processes in cells was NOT discovered by studying a virus?
c c c c c c c c c c c c c
a) the sequence of the genetic code.
c c c c c
b) DNA replication in both prokaryotic and eukaryotic cells
c c c c c c c
c) DNA is the genetic material
c c c c
d) regulation of gene expression in eukaryotic cells. c c c c c c
e) mRNA splicing in eukaryotic cells
c c c c
