Which of the four elements in proteins differentiates this class of substances from carbohydrates
and lipids correct answers a. carbon
Which of the proteins is soluble in water correct answers a. albumin b. alpha-globulins c. beta-
globulins d. gamma-globulins
How are amino acids joined together to form a chainlike structure correct answers a. disulfide
bonding b. hydrogen bonding c. covalent bonding d. peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to correct answers a. amino acid substitution
b. addition of a prosthetic group c. alteration in tertiary structure d. increase in solubility
Which aminoacidopathy is NOT caused by an enzyme deficiency correct answers a. maple syrup
disease b. phenylketonuria c. alkaptonuria d. cystinuria
How many immunoglobulin classes are currently recognized correct answers a. two b. three c.
four d. five
Which reagent is employed in the serum protein determination correct answers a. biuret b.
molybdenum blue c. ferricferrocyanide d. diacetyl monoxime
Why should hemolyzed serum not be used for total protein analysis correct answers a.
hemoglobin is absorbed at the same wavelength as protein in the reagent b. there are elevated
protein amounts in red blood cells as compared to serum c. hemoglobin reacts with biuret reagent
d. the value will be falsely lowered
,Which of the following is NOT true of the biuret reaction correct answers a. follows Beer's law
for a reasonable range of protein b. depends on the presence of at least two peptide linkages c. is
relatively free from interference by lipids and hemoglobin d. is insensitive to the low protein
levels of urine
Why is bromocresol purple the preferred indicator for albumin dye binding techniques correct
answers a. it lends itself to manual and automated procedures b. it never binds with alpha or beta
globulins c. it can be employed with both serum and plasma d. there is less interference from
pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6 correct answers a. albumin b. alpha-
globulins c. beta-globulins d. gamma-globulins
The total protein is 7.2" g/dL. The serum protein results are as follows: Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL Alpha2 11.0% Beta 17.6% 0.8 g/dL Gamma 14.8% What is the A/G ratio
correct answers a. 3.4 b. 1.6 c. 1.2 d. 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen
in which disease state correct answers a. acute inflammation b. monoclonal gammopathy c.
active cirrhosis d. nephrotic syndrome
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma-globulin, then what condition may exist in the patient correct answers
a. cirrhosis b. inflammation c. nephrotic syndrome d. monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance correct answers a. lipids
b. nucleic acids c. carbohydrates d. metals
All the following are glycoproteins EXCEPT which one correct answers a. ceruloplasmin b.
follicle-stimulating hormone c. fibrinogen d. cryoglobulin
, Which one of the following applies to cryoglobulins correct answers a. they are temperature-
sensitive proteins b. the specimen should clot at room temperature c. the specimen is incubated at
22°C for 24 hours d. only quantitative tests are available
Which of the following statements is true of albumin correct answers a. It makes up the smallest
portion of total protein. b. Its size prevents its passage through a damaged glomerulus. c. It is
produced in the liver and it is water soluble. d. Clinical problems are usually related to high
serum values.
Decreased alpha-fetoprotein values are associated with which condition correct answers a. Down
syndrome b. neural tube defects c. spina bifida d. cancer
Which one of the following proteins transports iron correct answers a. ceruloplasmin b.
hemopexin c. transferrin d. haptoglobin
In acute renal failure, which nonprotein nitrogen rises the fastest correct answers a. uric acid b.
creatinine c. ammonia d. blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product correct answers a.
ammonia b. nitrogen c. allantoin - d. carbon dioxide
Why can untreated urine be used for the determination of urea by the diacetyl monoxime method
correct answers a. Dialysis in an auto analyzer removes interfering substances. b. The method is
not measuring ammonia. c. The increased temperature destroys the ammonia. d. Zinc does not
interfere.
An elevated creatinine value is most likely to be accompanied by which of these values correct
answers a. normal uric acid b. elevated uric acid, 1 OX creatinine value c. normal blood urea
nitrogen d. elevated blood urea nitrogen, 1 OX creatinine value
What is the classical method for creatinine reaction correct answers a. Jaffe b. Ehrlich c. Kjeldahl
d. Nessler