BCH 330 Final Exam 2025 Latest Test Bank
With 600 Practice Questions and Correct
Answers | BCH 330 Final Practice Exam 2025|
BCH 330 Final Test Prep 2025 (New!)
Written in terms of friction during sedimentation,
f α s/ [m(1-Vbarc₀)], where s = _____ - ANSWER-Einstein-Smoluchowski,
sedimentation coefficient
G = ω²r
G = ___
ω = ___ of ___
r = ____ of particle from center - ANSWER-applied centrifugal field, angular
velocity, rotor, radial distance
_____ uses a high rotor speed and a long column to maximize the resolution. It
provides information regarding the size, shape, and molecular interactions. -
ANSWER-sedimentation velocity
M = s R T / [D(1 - Vbarρ₀)]
is used to determine the _________ by combining the Svedberg equation and the
Einstein relation - ANSWER-molecular mass
pg. 1
,When a ______ is spun in the presence of a heavy salt, the salt sets up a ______
and individual molecules will gather at the regions corresponding to their ______
(1/Vbar) - ANSWER-macromolecule, density gradient, density
In the ________, it was determined that the mode of DNA replication is _______
using _______ and _____. - ANSWER-Messelson-Stahl experiment,
semiconservative, ¹⁴N- and ¹⁵N-labeled nucleotides, density gradient centrifugation
When moving through a _____ (e.g., agarose or polyacrylamide), a particle will
reach a steady-state velocity (u). - ANSWER-support medium
The velocity of a singly charged particle at the given field leads to a _____.
µ = u/E - ANSWER-electrophoretic mobility (µ)
To determine the _____ of a molecule based on the results of gel electrophroesis, a
plot of _______ is created. - ANSWER-molecular weight, log MW vs. mobility
Using ____ one can study the binding of a given ligand to its acceptor (lattice)
molecule based on how the more mobile molecular is converted to the less mobile
bound species. The respective amount of each species (bound and free/more
mobile and less mobile) is determined from _____. - ANSWER-DNA mobility-
shift assay, electrophoretic band intensities
When performing ______, a protein is coated and it unfolds to form a linear shape.
As a result, proteins differ in length only. Importantly, all of them are
overwhelmingly coated with the anionic SDS, so all proteins migrate to the
positive electrode/reservoir. - ANSWER-SDS PAGE
pg. 2
,In ______, a strip of solid medium is coated with a series of ___ solutions, which
buffer he pH in each respective segment of the strip to maintain a specified pH
value. This technique is based on ___, not molecular weight.
Protein samples are placed in the middle of the strip and then electrophoresed. The
protein migrates toward the pH where its net charge becomes zero. The pH at
which the protein band focuses (charge = 0) is called the ____. - ANSWER-
isoelectric focusing, ampholyte, charge, isoelectric point
____________ are territorially bound to the DNA backbone phosphates, but free to
translate along the backbone. Creates a ____. - ANSWER-condensed counterions,
metastable DNA duplex
The following equation is ______:
∆²ψ = -4Πρ/ε
ε = dielectric constant
∆² = Laplacian constant - ANSWER-ρε Poisson equation
When two ends of a linear DNA are joined to the other two ends to produce a
circularized species, the ends cannot rotate with respect to each other, creating a
_____. The DNA may be forced to adopt a higher level of structure, referred to as
______.
The number of times one strand passes around the other is a constant that cannot
change called the ______. The energy can be apportioned into the ____ of one
strand about the other or the ____ of the duplex axis in space. - ANSWER-
topological constraint, supercoils, linking number, twisting, writhing
pg. 3
, A _____ protein known as ____ contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense _____ fluorophore. This protein is
remarkably useful because the fluorophore is _____. Importantly, GFP can be
attached to form a ____ with a second protein of interest during standard cloning
procedures. - ANSWER-β-barrel, GFP, intrinsic, encoded in the amino acid
sequence, fusion protein
_____ is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term _______ refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the _____ in a later called the _______, which has a reduced pH. - ANSWER-
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
The following equation is the analytical version of the principle of
______________________
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] - ANSWER-mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? - ANSWER-hydrophobic effect
What does ∆G stand for? - ANSWER-standard state free energy
What is calculated by the following equation?
pg. 4
With 600 Practice Questions and Correct
Answers | BCH 330 Final Practice Exam 2025|
BCH 330 Final Test Prep 2025 (New!)
Written in terms of friction during sedimentation,
f α s/ [m(1-Vbarc₀)], where s = _____ - ANSWER-Einstein-Smoluchowski,
sedimentation coefficient
G = ω²r
G = ___
ω = ___ of ___
r = ____ of particle from center - ANSWER-applied centrifugal field, angular
velocity, rotor, radial distance
_____ uses a high rotor speed and a long column to maximize the resolution. It
provides information regarding the size, shape, and molecular interactions. -
ANSWER-sedimentation velocity
M = s R T / [D(1 - Vbarρ₀)]
is used to determine the _________ by combining the Svedberg equation and the
Einstein relation - ANSWER-molecular mass
pg. 1
,When a ______ is spun in the presence of a heavy salt, the salt sets up a ______
and individual molecules will gather at the regions corresponding to their ______
(1/Vbar) - ANSWER-macromolecule, density gradient, density
In the ________, it was determined that the mode of DNA replication is _______
using _______ and _____. - ANSWER-Messelson-Stahl experiment,
semiconservative, ¹⁴N- and ¹⁵N-labeled nucleotides, density gradient centrifugation
When moving through a _____ (e.g., agarose or polyacrylamide), a particle will
reach a steady-state velocity (u). - ANSWER-support medium
The velocity of a singly charged particle at the given field leads to a _____.
µ = u/E - ANSWER-electrophoretic mobility (µ)
To determine the _____ of a molecule based on the results of gel electrophroesis, a
plot of _______ is created. - ANSWER-molecular weight, log MW vs. mobility
Using ____ one can study the binding of a given ligand to its acceptor (lattice)
molecule based on how the more mobile molecular is converted to the less mobile
bound species. The respective amount of each species (bound and free/more
mobile and less mobile) is determined from _____. - ANSWER-DNA mobility-
shift assay, electrophoretic band intensities
When performing ______, a protein is coated and it unfolds to form a linear shape.
As a result, proteins differ in length only. Importantly, all of them are
overwhelmingly coated with the anionic SDS, so all proteins migrate to the
positive electrode/reservoir. - ANSWER-SDS PAGE
pg. 2
,In ______, a strip of solid medium is coated with a series of ___ solutions, which
buffer he pH in each respective segment of the strip to maintain a specified pH
value. This technique is based on ___, not molecular weight.
Protein samples are placed in the middle of the strip and then electrophoresed. The
protein migrates toward the pH where its net charge becomes zero. The pH at
which the protein band focuses (charge = 0) is called the ____. - ANSWER-
isoelectric focusing, ampholyte, charge, isoelectric point
____________ are territorially bound to the DNA backbone phosphates, but free to
translate along the backbone. Creates a ____. - ANSWER-condensed counterions,
metastable DNA duplex
The following equation is ______:
∆²ψ = -4Πρ/ε
ε = dielectric constant
∆² = Laplacian constant - ANSWER-ρε Poisson equation
When two ends of a linear DNA are joined to the other two ends to produce a
circularized species, the ends cannot rotate with respect to each other, creating a
_____. The DNA may be forced to adopt a higher level of structure, referred to as
______.
The number of times one strand passes around the other is a constant that cannot
change called the ______. The energy can be apportioned into the ____ of one
strand about the other or the ____ of the duplex axis in space. - ANSWER-
topological constraint, supercoils, linking number, twisting, writhing
pg. 3
, A _____ protein known as ____ contains 3 AMINO ACIDS that undergo an
oxidative reaction to form an intense _____ fluorophore. This protein is
remarkably useful because the fluorophore is _____. Importantly, GFP can be
attached to form a ____ with a second protein of interest during standard cloning
procedures. - ANSWER-β-barrel, GFP, intrinsic, encoded in the amino acid
sequence, fusion protein
_____ is a support medium that has two functions: 1) prevents molecules from
diffusing into broad bands 2) provides a sieving material that obstructs the
movement of larger particles more than smaller ones, allowing them to separate.
The term _______ refers to a specific gel layer technique that "focuses" a protein
band. The mechanism involves creating a large voltage gradient between Cl- and
the _____ in a later called the _______, which has a reduced pH. - ANSWER-
Polyacrylamide, discontinuous gel, glycinate zwitterion, stacking gel
The following equation is the analytical version of the principle of
______________________
the rate of a chemical reaction is directly proportional to the product of the
activities or concentrations of the reactants
∆G= ∆G₀ + RTlnQ, where Q=[C][D]/[A][B] - ANSWER-mass action
What emphasizes the relative contributions of enthalpic and entropic energies to
the stabilization of globular proteins? - ANSWER-hydrophobic effect
What does ∆G stand for? - ANSWER-standard state free energy
What is calculated by the following equation?
pg. 4
