PHCH 667 Exam 1 Questions and Answers
100% Correct
What is the impact of disease prevalence on PPV and NPV? as disease prevalence
decreases:
PPV: DECREASES
NPV: INCREASE
What are some examples of mishandling clinical samples and laboratory errors that effect test
results? -improper storage/handling
-improper timing of sample collection
-faulty reagents
-controls not working correctly
-technical problems with instruments
-improper calibration
-calculation errors
,What are homogeneous samples? mixture where no separation step is required
What are heterogenous samples? mixture where a separation step is required
What are the principles of UV/Vis spec? the concentration of an analyte solution can be
determined by measuring absorbance at a defined wavelength
What are the principles of nephelometry measurement? measures turbidity of a sample
What does a pH meter measure? measures the pH which is the hydrogen-ion
concentration by taking the difference between two electrodes
What are some examples of techniques used to separate samples prior to analysis? -
centrifugation
-filtration
-dialysis
-extraction
, What properties of a protein does SDS-PAGE and IEF measure? PAGE: proteins based on
size and mass
IEF: based on charge
Columns used in liquid chromotography can separate biomolecules based on what physical
properties? size, charge, adsorption, specific binding
What are the principles of a mass spec measurement? measures molecular weight based
on hydrogen ions
What are some advantages of mass spec for analytical analysis? -universal
-fast
-high selectivity
What is an enzyme and why are they useful for clinical chemistry assays? proteins with a
specific 3D shape that act as biological catalysts
100% Correct
What is the impact of disease prevalence on PPV and NPV? as disease prevalence
decreases:
PPV: DECREASES
NPV: INCREASE
What are some examples of mishandling clinical samples and laboratory errors that effect test
results? -improper storage/handling
-improper timing of sample collection
-faulty reagents
-controls not working correctly
-technical problems with instruments
-improper calibration
-calculation errors
,What are homogeneous samples? mixture where no separation step is required
What are heterogenous samples? mixture where a separation step is required
What are the principles of UV/Vis spec? the concentration of an analyte solution can be
determined by measuring absorbance at a defined wavelength
What are the principles of nephelometry measurement? measures turbidity of a sample
What does a pH meter measure? measures the pH which is the hydrogen-ion
concentration by taking the difference between two electrodes
What are some examples of techniques used to separate samples prior to analysis? -
centrifugation
-filtration
-dialysis
-extraction
, What properties of a protein does SDS-PAGE and IEF measure? PAGE: proteins based on
size and mass
IEF: based on charge
Columns used in liquid chromotography can separate biomolecules based on what physical
properties? size, charge, adsorption, specific binding
What are the principles of a mass spec measurement? measures molecular weight based
on hydrogen ions
What are some advantages of mass spec for analytical analysis? -universal
-fast
-high selectivity
What is an enzyme and why are they useful for clinical chemistry assays? proteins with a
specific 3D shape that act as biological catalysts
