Cytogenic Techniques (Array CGH, FISH, Karyotype) Exam 100% Complete Solution 2025 A+ Graded

Cytogenic Techniques (Array CGH, FISH, Karyotype) Exam 100% Complete Solution 2025 A+ Graded 1. Cytogenetic techniques: Techniques used to interrogate the genome at the chromosomal level 2. Types of Cytogenic Techniques: 1. Array comparative genomic hybridisation (Array CGH): this technique uses an array-based approach to interrogate the chro mosomes at high resolution 2. Fluorescent in situ hybridisation (FISH): a targeted approach to investigate spe cific chromosome regions 3. Conventional Karyotyping with Light Microscopy: Largely superseded by array CGH, but is a highly skilled technique of visualising and analysing chromosomes using a light microscope 3. Array Comparative Genomic Hybridisation (Array CGH): Array CGH identifies gains or losses in the chromosomal material by the comparison of patient DNA with a reference DNA 4. Array: a glass slide where thousands of oligonucleotide probes are spotted 5. Oligonucleotides: sections of DNA and are distributed throughout the genome with some areas (i.e. important genes) having more densely packed probes to ensure better coverage in these areas! 6. Array CGH Method: 1. Hybridisation: Two DNAs are mixed together and hy....