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Summary ASSIGNMENT 2C APPLIED SCIENCE

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BTEC Assignment Brief
Pearson BTEC Level 3 National Certificate in Applied Science
Pearson BTEC Level 3 National Extended Certificate in
Applied Science Pearson BTEC Level 3 National Foundation
Qualification Diploma in Applied Science Pearson BTEC Level 3 National
Diploma in Applied Science
Pearson BTEC Level 3 National Extended Diploma in Applied
Science
Unit number and title
Unit 2: Practical Scientific Procedures and Techniques

Learning aim(s) (For NQF C: Undertake chromatographic techniques to identify
only) components in mixtures.
Assignment title Separate to identify



You are a newly appointed technical assistant at a large
chemical plant, Chemcalequip. As part of your induction
period and to progress in your role, you have to demonstrate
skills in a range of practical procedures and techniques. The
Vocational Scenario or company is often required to identify substances.
Context You need to demonstrate that you can carry out different
chromatographic techniques to separate and identify
components in mixtures. In a report of the techniques you
have used, you will need to explain the techniques, analyse
and evaluate your results and suggest improvements.


Task 1 Evaluate the chromatographic techniques you have
used in relation to the outcomes.
Demonstrate safe working practices and a high level of
proficiency when using the following chromatography
techniques;
 paper chromatography of extracted plant pigments
 Thin layer chromatography, TLC, of extracted plant
pigments
 paper chromatography of amino acids.
Repeat any separations where you can justify a lack of
satisfaction with the quality of the separation obtained.
Produce a report to include :
 an evaluation and analysis of the links between the
chromatography techniques used and the
chromatograms produced.
 conclusions about the components and their effect on
separation leading to the results obtained.
 results from the paper chromatography and TLC of
extracted plant pigments and from paper
chromatography of amino acids
 calculations of Rf values and consideration of the
factors that influence separation.
 an explanation of the principles behind the
chromatographic separations and their suitability.

,  justification of conclusions about the identification of
components in the mixtures, e.g. polarity of the
solvents, effect of molecule size on its mobility.
 Suggestions and rationales for specific improvements
to the chromatographic procedures carried out and
full justification of these suggestions.
Checklist of evidence A report on the experiments you have carried out.
required
An observation report with a checklist, completed by the
tutor, identifying the level of independence and proficiency
demonstrated whilst carrying out each technique. Safe
working practices must be identified.
Criteria covered by this task:
Unit/Criteria
To achieve the criteria you must show that you are able to:
reference
Evaluate the chromatographic techniques used in relation to outcomes
C.D3
and suggest improvements.
Analyse own chromatograms and relate the factors that affect the
C.M3
separation of mixtures to the quality of results obtained.
C.P5 Correctly use chromatographic techniques to produce chromatograms.

C.P6 Explain the use of chromatographic techniques to separate mixtures.

Sources of information http://www.virtlab.com/
to support you with this http://www.chemguide.co.uk/analysis/chromatography/
Assignment thinlayer.html#top

Above are some examples of websites. Further
useful resources may be found at:

http://qualifications.pearson.com/en/support/
published-resources.html#step1

Other assessment eg, work sheets, risk assessments, case study
materials attached to
this Assignment Brief




UNDERTAKING CHROMATOGRAPH TECHNIQUES

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