MOLECULAR DIAGNOSTICS EXAM 2
WITH COMPLETE SOLUTIONS
Enzyme that digests nucleic acid that starts at 5' or 3' and extends inward - ANSWER-
Exonuclease
Enzyme that digests nucleic acid at a specific sequence within the DNA molecule -
ANSWER-Endonuclease
A virus that infects bacteria - ANSWER-Bacteriophage
Produced by bacteria and help to protect against infection by bacteriophage -
ANSWER-Restriction Endonuclease
True or False: Restriction endonucleases will work on all DNA regardless of source -
ANSWER-True
How many type of Restriction Enzymes are there? - ANSWER-3
Which is the most useful Restriction enzyme for molecular techniques? - ANSWER-
Type II
What is the function of restriction enzymes in molecular biology? - ANSWER-"Molecular
Scissors"
How many base pairs do restriction enzymes recognize? - ANSWER-4 to 6 base pairs
in palindromic sequences
What factors determine how a restriction enzyme is identified? - ANSWER--Bacterial
genus & species
-Strain
-Enzyme number
What are the 2 "cuts" that restriction enzymes will make in the DNA? -
ANSWER--"Sticky" End
-"Blunt" End
Restriction enzyme that makes a "blunt" cut between the G and C in:
5-GGCC-3' - ANSWER-HAE III
HAE III restriction enzyme is derived from what micro-organism? - ANSWER-
Haemophilus aegypticus
, Restriction enzyme that makes a "blunt" cut between the G and C in:
5'-CAGCTG-3' - ANSWER-Pvu II
Pvu II restriction enzyme is derived from what micro-organism? - ANSWER-Proteus
vulgaris
Restriction enzyme that makes a "sticky" cut between the A and G in:
5'-GAATTC-3' - ANSWER-Eco R1 Enzyme
Eco R1 restriction enzyme is derived from what micro-organism? - ANSWER-E. coli
Restriction enzyme that makes a "sticky" cut between the G and A in:
5'-GACGTC-3' - ANSWER-Pst I Enzyme
Pst I restriction enzyme is derived from what micro-organism? - ANSWER-Providencia
stuartii
Restriction-Fragment Length Polymorphisms (RFLPs) - ANSWER-DNA fragments
produced by restriction enzymes
What are RFLPs used for? - ANSWER--DNA profiling
-Diagnosing genetic diseases
-Micro-organism fingerprinting
Positively charged gel electrophoresis terminal? - ANSWER-Anode
Negatively charged particle that migrates toward anode - ANSWER-Anions
Positively charged particle that migrates toward cathode - ANSWER-Cat-ions
What is the standard method used to separate, identify, & purify DNA fragments? -
ANSWER-Electrophoresis
At neutral pH (7.0 - 8.0), what polarity does DNA have? - ANSWER-Negative
What is the principle of DNA separation based on? - ANSWER-The size (length) of the
nucleic acid
If a DNA strand is short, would there be more or less recognition sites along the strand?
- ANSWER-More
What is the average distance between cuts? - ANSWER-4^n, where n is the number of
base pairs in the recognition site
Extrachromosomal DNA - found in many bacteria - ANSWER-Plasmid
WITH COMPLETE SOLUTIONS
Enzyme that digests nucleic acid that starts at 5' or 3' and extends inward - ANSWER-
Exonuclease
Enzyme that digests nucleic acid at a specific sequence within the DNA molecule -
ANSWER-Endonuclease
A virus that infects bacteria - ANSWER-Bacteriophage
Produced by bacteria and help to protect against infection by bacteriophage -
ANSWER-Restriction Endonuclease
True or False: Restriction endonucleases will work on all DNA regardless of source -
ANSWER-True
How many type of Restriction Enzymes are there? - ANSWER-3
Which is the most useful Restriction enzyme for molecular techniques? - ANSWER-
Type II
What is the function of restriction enzymes in molecular biology? - ANSWER-"Molecular
Scissors"
How many base pairs do restriction enzymes recognize? - ANSWER-4 to 6 base pairs
in palindromic sequences
What factors determine how a restriction enzyme is identified? - ANSWER--Bacterial
genus & species
-Strain
-Enzyme number
What are the 2 "cuts" that restriction enzymes will make in the DNA? -
ANSWER--"Sticky" End
-"Blunt" End
Restriction enzyme that makes a "blunt" cut between the G and C in:
5-GGCC-3' - ANSWER-HAE III
HAE III restriction enzyme is derived from what micro-organism? - ANSWER-
Haemophilus aegypticus
, Restriction enzyme that makes a "blunt" cut between the G and C in:
5'-CAGCTG-3' - ANSWER-Pvu II
Pvu II restriction enzyme is derived from what micro-organism? - ANSWER-Proteus
vulgaris
Restriction enzyme that makes a "sticky" cut between the A and G in:
5'-GAATTC-3' - ANSWER-Eco R1 Enzyme
Eco R1 restriction enzyme is derived from what micro-organism? - ANSWER-E. coli
Restriction enzyme that makes a "sticky" cut between the G and A in:
5'-GACGTC-3' - ANSWER-Pst I Enzyme
Pst I restriction enzyme is derived from what micro-organism? - ANSWER-Providencia
stuartii
Restriction-Fragment Length Polymorphisms (RFLPs) - ANSWER-DNA fragments
produced by restriction enzymes
What are RFLPs used for? - ANSWER--DNA profiling
-Diagnosing genetic diseases
-Micro-organism fingerprinting
Positively charged gel electrophoresis terminal? - ANSWER-Anode
Negatively charged particle that migrates toward anode - ANSWER-Anions
Positively charged particle that migrates toward cathode - ANSWER-Cat-ions
What is the standard method used to separate, identify, & purify DNA fragments? -
ANSWER-Electrophoresis
At neutral pH (7.0 - 8.0), what polarity does DNA have? - ANSWER-Negative
What is the principle of DNA separation based on? - ANSWER-The size (length) of the
nucleic acid
If a DNA strand is short, would there be more or less recognition sites along the strand?
- ANSWER-More
What is the average distance between cuts? - ANSWER-4^n, where n is the number of
base pairs in the recognition site
Extrachromosomal DNA - found in many bacteria - ANSWER-Plasmid
