2024/2025 MICROBIOLOGY LAB PRACTICAL
(A.BRADER) EXAM WITH CORRECT ANSWERS
1. Agar: a solidifying agent
100 degrees C
that liquefies at what
tem- perature?
2. Agar, in a liquid state,
40 degrees C
so- lidifies at what
tempera- ture?
3. Fermentation is a meta-
bolic process that Low to zero oxygen concentrations
occurs in what
conditions?
4. What is the purpose of To create NAD+
fermentation?
5. RODAC plates are Replicate Organism Detection and
agar plates; the Counting
acronym stands for:
6. CFU's stands for: Colony Forming Units
1/
, 2024/2025 MICROBIOLOGY LAB PRACTICAL
(A.BRADER) EXAM WITH CORRECT ANSWERS
7. The purpose of using ONLY VIABLE microorganisms
plate counts (RODAC
and swab testing) is to
deter- mine:
8. Aseptic technique is exclude contaminants from testing
used to:
9. A heat fix is performed to: 1. adhering specimen to slide
2. killing bacteria
3. denatures bacteria proteins to be able
to take up stain and view close to
size/shape
10.Basic stains usually it is attracted to the negatively charged
stained with bacteria and stains the bacteria
positively charge
cation or chro-
mophore because:
11. a bacterial smear from an placing a loop of water on a slide and
transferring
agar colony (solid another loop of bacteria (VERY small
media) is made by: amount) and mixing it in with water - air
dry - and heat fix.
12. Simple Stain allows the scientist to view the
microorganism by
increasing the refractive index of the
microorgan- ism compared to the
2/
(A.BRADER) EXAM WITH CORRECT ANSWERS
1. Agar: a solidifying agent
100 degrees C
that liquefies at what
tem- perature?
2. Agar, in a liquid state,
40 degrees C
so- lidifies at what
tempera- ture?
3. Fermentation is a meta-
bolic process that Low to zero oxygen concentrations
occurs in what
conditions?
4. What is the purpose of To create NAD+
fermentation?
5. RODAC plates are Replicate Organism Detection and
agar plates; the Counting
acronym stands for:
6. CFU's stands for: Colony Forming Units
1/
, 2024/2025 MICROBIOLOGY LAB PRACTICAL
(A.BRADER) EXAM WITH CORRECT ANSWERS
7. The purpose of using ONLY VIABLE microorganisms
plate counts (RODAC
and swab testing) is to
deter- mine:
8. Aseptic technique is exclude contaminants from testing
used to:
9. A heat fix is performed to: 1. adhering specimen to slide
2. killing bacteria
3. denatures bacteria proteins to be able
to take up stain and view close to
size/shape
10.Basic stains usually it is attracted to the negatively charged
stained with bacteria and stains the bacteria
positively charge
cation or chro-
mophore because:
11. a bacterial smear from an placing a loop of water on a slide and
transferring
agar colony (solid another loop of bacteria (VERY small
media) is made by: amount) and mixing it in with water - air
dry - and heat fix.
12. Simple Stain allows the scientist to view the
microorganism by
increasing the refractive index of the
microorgan- ism compared to the
2/
