ASCP BOC Hematology

____________ and ____________ are required for heme synthesis - answeriron and protoporphyrin Fe Transport protein - answerTransferrin Major Fe storage form - answerFerritin H2O insoluble Fe storage form (long-term) - answerHemosiderin Excess iron that is stored in tissues and body organs can lead to ____________ and ____________ - answerhemosiderosis and hemochromatosis (organ damage) What are the heme precursors (in order) - answerDelta, Porphobilinogen, UR, COP, PROTO EDTA chelates ____ - answerCalcium What are some sources of error for hemoglobin? - answericterus, lipemia, hemolysis, increased white count What are the hemoglobin reference ranges for men and women? - answermen: 16 +- 2 g/dL women: 14 +- 2 g/dL What happens in methemoglobin? - answerFe2+ is oxidized to Fe3+, cannot bind O2, is brown Measures packed cell volume in a percent - answerhematocrit What are some sources of error from a calculated hematocrit? - answercold agglutinins, high white count What are the hematocrit reference ranges for men and women? - answermale: 47 +- 5 % women: 42 +- 5% What is the calculation for MCV? - answer(HCT/RBC) x 10 What is the calculation for MCH? - answer(HB/RBC) x 10 What is the calculation for MCHC? - answer(HB/HCT) x 100 What is the reference range for MCV? - answer80 - 100 fL What is the reference range for MCH? - answer28 - 32 pg What is the reference range for MCHC? - answer32 - 36 % What indicates anisocytosis? - answerif the RDW is greater than 14.5% What are the red cell reference ranges for men and women? - answermen: 4.5 - 6.1 E6/uL women: 3.8 - 5.2 E6/uL What stain do we use for a blood smear exam? - answerwright stain (romanowsky, eosin, methylene blue, and methanol) What white cell would be increased? Bacterial infections - answerneutrophils What white cell would be increased? viral infections - answerlymphocytes What white cell would be increased? Tb, syphillis, malignancies - answermonocytes What white cell would be increased? Allergies, parasites - answereosinophils What white cell would be increased? immediate hypersensitivity - answerbasophils Hypersegmented neutrophils are associated with - answermegaloblastic anemia, B12 and folate deficiency Hyposegmented neutrophils are associated with - answerpelger huet anomaly (AML, AIDS) Toxic granulation and vacuoles are associated with - answerbacterial infection, burns, chemo Dhole bodies are associated with - answerbacterial infections, burns, may hegglin anomaly Variant lymphs are associated with - answerIM, viral infection What is the calculation for a corrected white count when nucleolar reds are seen? - answer(WBC x 100/ 100 +nrbc) What RBC inclusion is NOT seen with wright stain? - answerHeinz bodies Where is blood produced at 1 month? - answeryolk sac Where is blood produced at 4 months? - answerspleen and liver Where is blood produced at 8 months? - answerbone marrow What hemoglobin chains make up A2? - answeralpha, delta What hemoglobin chins make up A? - answeralpha, beta What hemoglobin chains make up F? - answeralpha, gamma What hemoglobin chains make up portland? - answerzeta, gamma