Blood Bank Practical Questions with complete Solutions Graded A+
What type of virus is CMV? - Answers DNA herpesvirus
What virus is carried only in blood components with viable cells? - Answers CMV
Do you need to have CMV seropositivity to transmit CMV? - Answers no
Premature babies, stem cell/marrow tx recip, solid organ tx recip (esp renal), and AIDS patients are at
risk for infxn for which virus? - Answers CMV
What types of products do you give to decrease risk of CMV? - Answers CMV seronegative donors
Leukoreduced
What is the max # of WBC that can be in leukoreduced products? - Answers <5 x 10^6 WBCs (will pretty
much eliminate CMV transmission)
What type of blood do you give if blood needed in less than 10 min (NOW!) - Answers uncrossmatched
group O neg
(may give male or old female O pos)
What type of blood do you give if you need blood in 10-30min? - Answers Uncrossmatched ABO group
and Rh type specific
What type of blood do you give if you need blood over 30min? - Answers Crossmatched ABO group and
Rh type specific
Who approves switching ABO and/or Rh types? - Answers medical director
When do you want to switch patient ABO/Rh types? - Answers safest to wait until incompatible plasma
has cleared
(ie pt type A siwtched to O can carry a lot of anti-A)
What type of RhIg do you want to give to females? - Answers IV form (WinRho)
What is the term for tx of amt of blood that equals pt's blood vol in 24h? - Answers massive transfusion
How many units of blood does a person get in 24h in a massive tx? - Answers 10-20 units of blood
What % of pt's blood vol is transfusion in 3hrs in a massive tx? - Answers 50%
Does 2,3 DPG go up or down in stored blood? - Answers down (Hgb hold tight to O2)
, Does pH go up or down in stored blood? - Answers down (dec pH due to glycolysis)
Does ATP go up or down in stored blood? - Answers down
Do coag fx go up or down in stored blood? - Answers down
Does K go up or down in stored blood? - Answers up
Does free Hb go up or down in stored blood? - Answers up
What is the term for partial press of O2 where Hb is 50% saturated? - Answers p50
If p50 is DECREASED which way does the O2 curve shift? - Answers To the LEFT
If p50 is DECREASED does O2 affinity for Hb go up or down? - Answers INCREASED O2 affinity
How many days does it take to get 2,3 DPG to be zero in blood pdcts? - Answers 14 days
When the O2 curve shifts to the RIGHT does the p50 inc or dec? - Answers INCREASE
When the p50 is increased what happens to the O2 affinity? - Answers decreases (p50 is opp of O2
affinity)
What is the most important consideration in tx SOLID organs? - Answers ABO
What is the most important consideration in tx of PBSC? - Answers HLA
For group A or O pts for solid organ tx what type of blood do you give? - Answers ABO identical RBCs
For group AB or B in solid organ tx what type of blood do you give? - Answers Group O RBCs can be used
Can you give AB pt A RBC with A FFP? - Answers yes (AB FFP is uncommon)
What is the main concern in renal tx? - Answers CMV
T or F the most common causes of plt refractoriness are nonimmune (ie fever, splenomegaly, DIC,
bleeding) - Answers True
True or F the most common cause of plt refractoriness is immune (ie HLA or plt Ab)? - Answers False,
nonimmune causes are more common
Do you want to give fresher or older plt to pt's who are refractory to plt? - Answers fresher, ABO
identical products
What test do you do to check for immune causes to plt refractoriness? - Answers Plt Ab screen
What is important to match on plt? - Answers HLA
Which HLA class do you want to have the best match with in plt? - Answers HLA Class I
What type of virus is CMV? - Answers DNA herpesvirus
What virus is carried only in blood components with viable cells? - Answers CMV
Do you need to have CMV seropositivity to transmit CMV? - Answers no
Premature babies, stem cell/marrow tx recip, solid organ tx recip (esp renal), and AIDS patients are at
risk for infxn for which virus? - Answers CMV
What types of products do you give to decrease risk of CMV? - Answers CMV seronegative donors
Leukoreduced
What is the max # of WBC that can be in leukoreduced products? - Answers <5 x 10^6 WBCs (will pretty
much eliminate CMV transmission)
What type of blood do you give if blood needed in less than 10 min (NOW!) - Answers uncrossmatched
group O neg
(may give male or old female O pos)
What type of blood do you give if you need blood in 10-30min? - Answers Uncrossmatched ABO group
and Rh type specific
What type of blood do you give if you need blood over 30min? - Answers Crossmatched ABO group and
Rh type specific
Who approves switching ABO and/or Rh types? - Answers medical director
When do you want to switch patient ABO/Rh types? - Answers safest to wait until incompatible plasma
has cleared
(ie pt type A siwtched to O can carry a lot of anti-A)
What type of RhIg do you want to give to females? - Answers IV form (WinRho)
What is the term for tx of amt of blood that equals pt's blood vol in 24h? - Answers massive transfusion
How many units of blood does a person get in 24h in a massive tx? - Answers 10-20 units of blood
What % of pt's blood vol is transfusion in 3hrs in a massive tx? - Answers 50%
Does 2,3 DPG go up or down in stored blood? - Answers down (Hgb hold tight to O2)
, Does pH go up or down in stored blood? - Answers down (dec pH due to glycolysis)
Does ATP go up or down in stored blood? - Answers down
Do coag fx go up or down in stored blood? - Answers down
Does K go up or down in stored blood? - Answers up
Does free Hb go up or down in stored blood? - Answers up
What is the term for partial press of O2 where Hb is 50% saturated? - Answers p50
If p50 is DECREASED which way does the O2 curve shift? - Answers To the LEFT
If p50 is DECREASED does O2 affinity for Hb go up or down? - Answers INCREASED O2 affinity
How many days does it take to get 2,3 DPG to be zero in blood pdcts? - Answers 14 days
When the O2 curve shifts to the RIGHT does the p50 inc or dec? - Answers INCREASE
When the p50 is increased what happens to the O2 affinity? - Answers decreases (p50 is opp of O2
affinity)
What is the most important consideration in tx SOLID organs? - Answers ABO
What is the most important consideration in tx of PBSC? - Answers HLA
For group A or O pts for solid organ tx what type of blood do you give? - Answers ABO identical RBCs
For group AB or B in solid organ tx what type of blood do you give? - Answers Group O RBCs can be used
Can you give AB pt A RBC with A FFP? - Answers yes (AB FFP is uncommon)
What is the main concern in renal tx? - Answers CMV
T or F the most common causes of plt refractoriness are nonimmune (ie fever, splenomegaly, DIC,
bleeding) - Answers True
True or F the most common cause of plt refractoriness is immune (ie HLA or plt Ab)? - Answers False,
nonimmune causes are more common
Do you want to give fresher or older plt to pt's who are refractory to plt? - Answers fresher, ABO
identical products
What test do you do to check for immune causes to plt refractoriness? - Answers Plt Ab screen
What is important to match on plt? - Answers HLA
Which HLA class do you want to have the best match with in plt? - Answers HLA Class I
