BOC Blood Bank Questions with complete Solutions Rated A+

BOC Blood Bank Questions with complete Solutions Rated A+ The most widely accepted QC test to measure probable Hematopoietic Progenitor Cell (HPC) engraftment is: - Answers CD34+ cell enumeration is the best way to evaluate HPC engraftment In an emergency situation, what type of blood should be given to a female patient of child-bearing age if the ABO group and Rh type are unknown? - Answers Group O, Rh-negative red cells should be given Cryoprecipitated AHF must contain a minimum of how many international units of Factor VIII - Answers 80 IU NAT testing is required for this infectious agent - Answers HBV To screen for HBV what must happen - Answers Nucleic acid test required for each donation A patient's record shows a previous anti-Jkb, but the current antibody screen is negative. What further testing should be done before transfusion - Answers give Jk(b-), crossmatch compatible blood ABO-hemolytic disease of the newborn - Answers is usually seen only in the newborn of group O mothers Irradiation of a unit of Red Blood Cells is done to prevent the replication of donor - Answers lymphocytes Criteria determining Rh immune globulin eligibility include - Answers mother has not been previously immunized to the D antigen Which of the following practices has been useful in reducing the incidence of Transfusion Related Acute Lung Injury (TRALI) - Answers use of Fresh Frozen Plasma from male donors A mother is Rh-negative and the father Rh-positive. Their baby is Rh-negative. It may be concluded that - Answers the father is heterozygous for D A blood donor has the genotype: hh, AB. Using anti-A and anti-B antisera, the donor's red cells will type as group - Answers O Which characteristics are true of all 3 of the following antibodies: anti-Fya, anti-Jka, and anti-K? - Answers detected at IAT phase and may cause hemolytic disease of the fetus and newborn (HDFN) and transfusion reactions During initial investigation of a suspected hemolytic transfusion reaction, it was observed that Blood Bank paperwork and patient sample and blood component labels were correct, the posttransfusion reaction plasma was yellow as was the pretransfusion sample, and the direct antiglobulin test was negative. Repeat ABO typing on the posttransfusion sample confirmed the pretransfusion results. What is the next step in this investigation? - Answers no further serological testing is necessary Red Blood Cells, Leukocytes Reduced must be stored at - Answers 1-6°C Which of the following is a characteristic of anti-i? - Answers reacts best at room temperature or 4°C When untreated indicator cells are used in the rosette test, a positive result occurs when there are >6 rosettes/5 fields. In this case, the results indicate that the mother had a larger than normal (≥10 mL) fetal maternal hemorrhage (FMH). Additional testing needs to be performed to calculate the FMH size. - Answers mother has a larger than normal FMH The Donath-Landsteiner test is diagnostic for PCH. The antibody is IgG and is biphasic: hemolysis occurs when the antibody is incubated with cells and cold temperatures and then incubated at 37°C. Often the antibody demonstrates specificity towards the high-incidence antigen P (not to be confused with P1). The antibody screen is usually negative and the patient's red cells are coated with complement. - Answers paroxysmal cold hemoglobinuria (PCH) Which of the following phenotypes will react with anti-f? - Answers rr An antibody that causes in vitro hemolysis and reacts with the red cells of 3 out of 10 AHG-crossmatched donor units is most likely Lewis antibodies may bind complement and fresh serum that contains anti-Lea may hemolyze Le(a+) red cells in vitro. Approximately 22% of the population is Le(a+) - Answers anti-Lea Ax cells are more strongly reactive with anti-A,B than with anti-A and the plasma frequently has anti-A1 present. - Answers anti-A zero, anti-B zero, anti-AB 2+ Upon inspection, a unit of Apheresis Platelets is noted to have visible clots, but otherwise appears normal. The technologist should: - Answers quarantine for Gram stain and culture The most appropriate laboratory test for early detection of acute posttransfusion hemolysis is - Answers visual inspection for free plasma hemoglobin What is the most important consideration for a patient requiring red cell transfusion due to severe anemia when their serum contains a warm autoantibody - Answers determine the presence of underlying alloantibody(ies) For a patient who has suffered an acute hemolytic transfusion reaction, the primary treatment goal should be to - Answers reverse hypotension and minimize renal damage What antigens would be found in the saliva of an individual with the genotype Sese Lele AO HH Lea, Leb, A, H - Answers Due to the inheritance of the Leand Se genes, Lea and Leb antigens will be present in the saliva. Due to the inheritance of the Se, A,andH genes, A and H antigens will also be present. The test that is currently used to detect donors who are infected with the AIDS virus is - Answers anti-HIV-1/2 Which of the following is considered to be a high prevalence antigen - Answers Vel When results with anti-A, anti-B and anti-D are all positive, there is no way to determine the accuracy of the results. Repeat testing with an inert control, verified as negative, is the only way to confirm the accuracy of these results. - Answers Based on the results below, what is the next step in determination of the patient's ABO/Rh type? ABO hemolytic disease of the fetus and newborn (HDFN) differs from Rh HDFN in that - Answers Rh HDFN is clinically more severe than ABO HDFN How to differentiate between IgG and IgM - Answers Use DTT (dithiothreitol) will cleave IgM but leave IgG A patient demonstrates 4+ reactivity with all red cells tested and the autocontrol is nonreactive. This high incidence antibody was suspected to be related to the P1PK blood group system as the patient is the rare p phenotype. What antibody specificity should be suspected? - Answers anti-PP1Pk A group A, Rh-positive infant of a group O, Rh-positive mother has a weakly positive direct antiglobulin test and a moderately elevated bilirubin 12 hours after birth. The most likely cause is: - Answers ABO HDFN occurs most commonly in group A babies born to group O mothers and usually has a mild course. The DAT is typically weak or negative and jaundice develops 12 - 48 hours after birth. The mother and baby are both Rh-positive. anti-S - Answers When the red cells of an individual fail to react with anti-U, they usually fail to react with The red cells of a nonsecretor (se/se) will most likely type as - Answers Le(a+b-) Which of the following might cause a false-negative indirect antiglobulin test (IAT)? - Answers too heavy a cell suspension Which of the following antigens is most likely to be involved in hemolytic disease of the fetus and newborn? - Answers Kell A donor who has just donated 2 units of Apheresis Red Blood Cells will be deferred from further blood donation for a minimum of how many weeks? - Answers 16 weeks A Kleihauer-Betke stain of a postpartum blood film revealed 0.3% fetal cells. What is the