Blood Bank- Serology Exam Questions and Answers Latest Update Graded A+
The listed criteria are typical for serological calibration of a centrifuge. Optimum spin time is the least
amount of time when all criteria are satisfied. - Answers A serological centrifuge is recalibrated for ABO
testing after major repairs.
Time in seconds 15 20 25 30
is button delineated? yes yes yes yes
is supernatant clear? no yes yes yes
button easy to resuspend? yes yes yes no
strength of reaction? +m 1+ 1+ 1+
Given the data above, the centrifuge time for this machine should be:
a. 15 sec
b. 20 sec
c. 25 sec
d. 30 sec
a
Samples must be labeled with 2 independent patient identifiers and the date of collection. This
information should be identical to that on the patient's identification band and request. - Answers
Which of the following represents an acceptably identified patient for sample collection and
transfusion?
a. a handwritten band with patient's name and hospital identification number is affixed to the patient's
leg
b. the addressographed hospital band is taped to the patient's bed
c. an unbanded patient responds positively when his name is called
,d. the chart transported with the patient contains his armband not yet attached
d
Results of ABO and Rh testing on a current specimen must always be compared to that of a previous
transfusion period. Errors in typing or patient identification may be detected when discrepancies are
found. Collection of a new sample allows determination of which sample was incorrectly collected. -
Answers Samples from the same patient were received on 2 consecutive days. Test results are
summarized below:
Day #1 Day #2
anti-A 4+ 0
anti-B 0 4+
anti-D 3+ 3+
A1 cells 0 4+
B cells 4+ 0
Ab screen 0 0
How should the request for crossmatch be handled?
a. crossmatch A, Rh-positive units with sample from day 1
b. crossmatch B, Rh-positive units with sample from day 2
c. crossmatch AB, Rh-positive units with both samples
d. collect a new sample and repeat the tests
c
A serological test to confirm the ABO on all RBC units and Rh on units labeled as Rh-negative must be
performed prior to transfusion. Any errors in labeling must be reported to the collection facility -
Answers The following test results are noted for a unit of blood labeled group A, Rh-negative:
,Cells tested with:
anti-A anti-B anti-D
4+ 0 3+
What should be done next?
a. transfuse as a group A, Rh-negative
b. transfuse as a group A, Rh-positive
c. notify the collecting facility
d. discard the unit
c
Samples must be labeled with 2 independent patient identifiers and the date of collection. This
information should be identical to that on the patients identification band and request. There must be a
mechanism to identify the phlebotomist, but initialing the sample tubes is not required. - Answers What
information is essential on patient blood sample labels drawn for compatibility testing?
a. biohazard sticker for AIDS patient
b. patient's room number
c. unique patient medical number
d. phlebotomist initials
b
Granulocytes must be compatible with the recipient's serum. Granulocyte products have an expiration
of 24 hours. - Answers Granulocytes for transfusion should:
a. be administered through a microaggregate filter
b. be ABO compatible with the recipient's serum
, c. be infused within 72 hours of collection
d. never be transfused to patients with a history of febrile transfusion reactions
c
Because neonates are immunologically immature, alloimmunization to red cell antigens is very rare
during the neonatal period. No crossmatching is required if the initial antibody screen performed with
either the baby's or mother's plasma is negative. - Answers A neonate will be transfused for the first
time with group O red blood cells. Which of the following is appropriate compatibility testing?
a. crossmatch with mother's serum
b. crossmatch with baby's serum
c. no crossmatch is necessary if initial plasma screening is negative
d. no screening or crossmatching is necessary for neonates
b
A positive DAT will interfere with weak D testing causing both the patient and control to demonstrate
positive results. Any positive result in the control tube invalidates any results. - Answers A group B, Rh-
negative patient has a positive DAT. Which of the following situations would occur?
a. all major crossmatches would be incompatible
b. the weak D test and control would be positive
c. the antibody screening test would be positive
d. the forward and reverse ABO groupings would not agree
c
Patients with multiple myeloma demonstrate rouleaux formation, which can cause the appearance of
agglutination. If the cells are washed to remove residual plasma, and tests repeated, an accurate red cell
typing is obtained. By performing a saline replacement with the reverse typing, true agglutination will
The listed criteria are typical for serological calibration of a centrifuge. Optimum spin time is the least
amount of time when all criteria are satisfied. - Answers A serological centrifuge is recalibrated for ABO
testing after major repairs.
Time in seconds 15 20 25 30
is button delineated? yes yes yes yes
is supernatant clear? no yes yes yes
button easy to resuspend? yes yes yes no
strength of reaction? +m 1+ 1+ 1+
Given the data above, the centrifuge time for this machine should be:
a. 15 sec
b. 20 sec
c. 25 sec
d. 30 sec
a
Samples must be labeled with 2 independent patient identifiers and the date of collection. This
information should be identical to that on the patient's identification band and request. - Answers
Which of the following represents an acceptably identified patient for sample collection and
transfusion?
a. a handwritten band with patient's name and hospital identification number is affixed to the patient's
leg
b. the addressographed hospital band is taped to the patient's bed
c. an unbanded patient responds positively when his name is called
,d. the chart transported with the patient contains his armband not yet attached
d
Results of ABO and Rh testing on a current specimen must always be compared to that of a previous
transfusion period. Errors in typing or patient identification may be detected when discrepancies are
found. Collection of a new sample allows determination of which sample was incorrectly collected. -
Answers Samples from the same patient were received on 2 consecutive days. Test results are
summarized below:
Day #1 Day #2
anti-A 4+ 0
anti-B 0 4+
anti-D 3+ 3+
A1 cells 0 4+
B cells 4+ 0
Ab screen 0 0
How should the request for crossmatch be handled?
a. crossmatch A, Rh-positive units with sample from day 1
b. crossmatch B, Rh-positive units with sample from day 2
c. crossmatch AB, Rh-positive units with both samples
d. collect a new sample and repeat the tests
c
A serological test to confirm the ABO on all RBC units and Rh on units labeled as Rh-negative must be
performed prior to transfusion. Any errors in labeling must be reported to the collection facility -
Answers The following test results are noted for a unit of blood labeled group A, Rh-negative:
,Cells tested with:
anti-A anti-B anti-D
4+ 0 3+
What should be done next?
a. transfuse as a group A, Rh-negative
b. transfuse as a group A, Rh-positive
c. notify the collecting facility
d. discard the unit
c
Samples must be labeled with 2 independent patient identifiers and the date of collection. This
information should be identical to that on the patients identification band and request. There must be a
mechanism to identify the phlebotomist, but initialing the sample tubes is not required. - Answers What
information is essential on patient blood sample labels drawn for compatibility testing?
a. biohazard sticker for AIDS patient
b. patient's room number
c. unique patient medical number
d. phlebotomist initials
b
Granulocytes must be compatible with the recipient's serum. Granulocyte products have an expiration
of 24 hours. - Answers Granulocytes for transfusion should:
a. be administered through a microaggregate filter
b. be ABO compatible with the recipient's serum
, c. be infused within 72 hours of collection
d. never be transfused to patients with a history of febrile transfusion reactions
c
Because neonates are immunologically immature, alloimmunization to red cell antigens is very rare
during the neonatal period. No crossmatching is required if the initial antibody screen performed with
either the baby's or mother's plasma is negative. - Answers A neonate will be transfused for the first
time with group O red blood cells. Which of the following is appropriate compatibility testing?
a. crossmatch with mother's serum
b. crossmatch with baby's serum
c. no crossmatch is necessary if initial plasma screening is negative
d. no screening or crossmatching is necessary for neonates
b
A positive DAT will interfere with weak D testing causing both the patient and control to demonstrate
positive results. Any positive result in the control tube invalidates any results. - Answers A group B, Rh-
negative patient has a positive DAT. Which of the following situations would occur?
a. all major crossmatches would be incompatible
b. the weak D test and control would be positive
c. the antibody screening test would be positive
d. the forward and reverse ABO groupings would not agree
c
Patients with multiple myeloma demonstrate rouleaux formation, which can cause the appearance of
agglutination. If the cells are washed to remove residual plasma, and tests repeated, an accurate red cell
typing is obtained. By performing a saline replacement with the reverse typing, true agglutination will
